FTA-LAMP based biosensor for a rapid in-field detection of Globodera pallida—the pale potato cyst nematode
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2024 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/173112 |
Resumo: | Funding Information: The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by National Funds through FCT—Foundation for Science and Technology under the PhD fellowships BD 138724/2018 and COVID/BD/152764/2023, the project UIDB/05183/2020 (MED–Mediterranean Institute for Agriculture, Environment and Development, Institute for Advanced Studies and Research, Évora University) and the project UID/05367/2020 (PROGRAMATICO—Research Unit INESC MN, Microsystems and Nanotechnologies). Publication was supporteded by Bactometer, 2022-RIS_Innovation-068, funded through EIT Health. Funding Information: The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by National Funds through FCT—Foundation for Science and Technology under the PhD fellowships BD 138724/2018 and COVID/BD/152764/2023, the project UIDB/05183/2020 (MED–Mediterranean Institute for Agriculture, Environment and Development, Institute for Advanced Studies and Research, Évora University) and the project UID/05367/2020 (PROGRAMATICO—Research Unit INESC MN, Microsystems and Nanotechnologies). Publication was supporteded by Bactometer, 2022-RIS_Innovation-068, funded through EIT Health. Publisher Copyright: Copyright © 2024 Camacho, Albuquerque, Inácio, Martins, Mota, Freitas and de Andrade. |
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FTA-LAMP based biosensor for a rapid in-field detection of Globodera pallida—the pale potato cyst nematodeFTA-card ®Lab-on-a-chipLAMPmagnetoresistive biochipsPCNBiotechnologyBioengineeringHistologyBiomedical EngineeringFunding Information: The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by National Funds through FCT—Foundation for Science and Technology under the PhD fellowships BD 138724/2018 and COVID/BD/152764/2023, the project UIDB/05183/2020 (MED–Mediterranean Institute for Agriculture, Environment and Development, Institute for Advanced Studies and Research, Évora University) and the project UID/05367/2020 (PROGRAMATICO—Research Unit INESC MN, Microsystems and Nanotechnologies). Publication was supporteded by Bactometer, 2022-RIS_Innovation-068, funded through EIT Health. Funding Information: The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by National Funds through FCT—Foundation for Science and Technology under the PhD fellowships BD 138724/2018 and COVID/BD/152764/2023, the project UIDB/05183/2020 (MED–Mediterranean Institute for Agriculture, Environment and Development, Institute for Advanced Studies and Research, Évora University) and the project UID/05367/2020 (PROGRAMATICO—Research Unit INESC MN, Microsystems and Nanotechnologies). Publication was supporteded by Bactometer, 2022-RIS_Innovation-068, funded through EIT Health. Publisher Copyright: Copyright © 2024 Camacho, Albuquerque, Inácio, Martins, Mota, Freitas and de Andrade.The combination of a sensitive and specific magnetoresistive sensing device with an easy DNA extraction method and a rapid isothermal amplification is presented here targeting the on-site detection of Globodera pallida, a potato endoparasitic nematode. FTA-cards were used for DNA extraction, LAMP was the method developed for DNA amplification and a nanoparticle functionalized magnetic-biosensor was used for the detection. The combinatorial effect of these three emerging technologies has the capacity to detect G. pallida with a detection limit of one juvenile, even when mixed with other related species. This combined system is far more interesting than what a single technology can provide. Magnetic biosensors can be combined with any DNA extraction protocol and LAMP forming a new solution to target G. pallida. The probe designed in this study consistently distinguished G. pallida (∆Vacbinding/Vacsensor above 1%) from other cyst nematodes (∆Vacbinding/Vacsensor below 1%). It was confirmed that DNA either extracted with FTA-cards or Lab extraction Kit was of enough quantity and quality to detect G. pallida whenever present (alone or in mixed samples), ensuring probe specificity and sensitivity. This work provides insights for a new strategy to construct advanced devices for pathogens in-field diagnostics. LAMP runs separately but can be easily integrated into a single device.Bioresources 4 Sustainability (GREEN-IT)RUNCamacho, Maria JoãoAlbuquerque, Débora C.Inácio, Maria L.Martins, Verónica C.Mota, ManuelFreitas, Paulo P.de Andrade, Eugénia2024-10-07T22:21:36Z2024-01-182024-01-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/173112eng2296-4185PURE: 90684926https://doi.org/10.3389/fbioe.2024.1337879info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-10-14T01:39:26Zoai:run.unl.pt:10362/173112Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-10-14T01:39:26Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
FTA-LAMP based biosensor for a rapid in-field detection of Globodera pallida—the pale potato cyst nematode |
| title |
FTA-LAMP based biosensor for a rapid in-field detection of Globodera pallida—the pale potato cyst nematode |
| spellingShingle |
FTA-LAMP based biosensor for a rapid in-field detection of Globodera pallida—the pale potato cyst nematode Camacho, Maria João FTA-card ® Lab-on-a-chip LAMP magnetoresistive biochips PCN Biotechnology Bioengineering Histology Biomedical Engineering |
| title_short |
FTA-LAMP based biosensor for a rapid in-field detection of Globodera pallida—the pale potato cyst nematode |
| title_full |
FTA-LAMP based biosensor for a rapid in-field detection of Globodera pallida—the pale potato cyst nematode |
| title_fullStr |
FTA-LAMP based biosensor for a rapid in-field detection of Globodera pallida—the pale potato cyst nematode |
| title_full_unstemmed |
FTA-LAMP based biosensor for a rapid in-field detection of Globodera pallida—the pale potato cyst nematode |
| title_sort |
FTA-LAMP based biosensor for a rapid in-field detection of Globodera pallida—the pale potato cyst nematode |
| author |
Camacho, Maria João |
| author_facet |
Camacho, Maria João Albuquerque, Débora C. Inácio, Maria L. Martins, Verónica C. Mota, Manuel Freitas, Paulo P. de Andrade, Eugénia |
| author_role |
author |
| author2 |
Albuquerque, Débora C. Inácio, Maria L. Martins, Verónica C. Mota, Manuel Freitas, Paulo P. de Andrade, Eugénia |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Bioresources 4 Sustainability (GREEN-IT) RUN |
| dc.contributor.author.fl_str_mv |
Camacho, Maria João Albuquerque, Débora C. Inácio, Maria L. Martins, Verónica C. Mota, Manuel Freitas, Paulo P. de Andrade, Eugénia |
| dc.subject.por.fl_str_mv |
FTA-card ® Lab-on-a-chip LAMP magnetoresistive biochips PCN Biotechnology Bioengineering Histology Biomedical Engineering |
| topic |
FTA-card ® Lab-on-a-chip LAMP magnetoresistive biochips PCN Biotechnology Bioengineering Histology Biomedical Engineering |
| description |
Funding Information: The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by National Funds through FCT—Foundation for Science and Technology under the PhD fellowships BD 138724/2018 and COVID/BD/152764/2023, the project UIDB/05183/2020 (MED–Mediterranean Institute for Agriculture, Environment and Development, Institute for Advanced Studies and Research, Évora University) and the project UID/05367/2020 (PROGRAMATICO—Research Unit INESC MN, Microsystems and Nanotechnologies). Publication was supporteded by Bactometer, 2022-RIS_Innovation-068, funded through EIT Health. Funding Information: The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by National Funds through FCT—Foundation for Science and Technology under the PhD fellowships BD 138724/2018 and COVID/BD/152764/2023, the project UIDB/05183/2020 (MED–Mediterranean Institute for Agriculture, Environment and Development, Institute for Advanced Studies and Research, Évora University) and the project UID/05367/2020 (PROGRAMATICO—Research Unit INESC MN, Microsystems and Nanotechnologies). Publication was supporteded by Bactometer, 2022-RIS_Innovation-068, funded through EIT Health. Publisher Copyright: Copyright © 2024 Camacho, Albuquerque, Inácio, Martins, Mota, Freitas and de Andrade. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024-10-07T22:21:36Z 2024-01-18 2024-01-18T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/173112 |
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http://hdl.handle.net/10362/173112 |
| dc.language.iso.fl_str_mv |
eng |
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eng |
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2296-4185 PURE: 90684926 https://doi.org/10.3389/fbioe.2024.1337879 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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