A Lab-on-a-Chip Approach for the Detection of the Quarantine Potato Cyst Nematode Globodera pallida

Detalhes bibliográficos
Autor(a) principal: Camacho, Maria João
Data de Publicação: 2023
Outros Autores: Albuquerque, Débora C., de Andrade, Eugénia, Martins, Verónica C., Inácio, Maria L., Mota, Manuel, Freitas, Paulo P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158474
Resumo: Funding Information: This research was funded by National Funds through the FCT—Foundation for Science and Technology under the Ph.D. fellowships BD 138724/2018, the project UIDB/05183/2020 (MED–Mediterranean Institute for Agriculture, Environment and Development, Institute for Advanced Studies and Research, Évora University) and the project UID/05367/2020 (PROGRAMATICO—Research Unit INESC MN, Microsystems and Nanotechnologies). Publisher Copyright: © 2023 by the authors.
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spelling A Lab-on-a-Chip Approach for the Detection of the Quarantine Potato Cyst Nematode Globodera pallidaasymmetric PCRmagnetoresistive biochipsPCNAnalytical ChemistryInformation SystemsAtomic and Molecular Physics, and OpticsBiochemistryInstrumentationElectrical and Electronic EngineeringFunding Information: This research was funded by National Funds through the FCT—Foundation for Science and Technology under the Ph.D. fellowships BD 138724/2018, the project UIDB/05183/2020 (MED–Mediterranean Institute for Agriculture, Environment and Development, Institute for Advanced Studies and Research, Évora University) and the project UID/05367/2020 (PROGRAMATICO—Research Unit INESC MN, Microsystems and Nanotechnologies). Publisher Copyright: © 2023 by the authors.The potato cyst nematode (PCN), Globodera pallida, has acquired significant importance throughout Europe due to its widespread prevalence and negative effects on potato production. Thus, rapid and reliable diagnosis of PCN is critical during surveillance programs and for the implementation of control measures. The development of innovative technologies to overcome the limitations of current methodologies in achieving early detection is needed. Lab-on-a-chip devices can swiftly and accurately detect the presence of certain nucleotide sequences with high sensitivity and convert the presence of biological components into an understandable electrical signal by combining biosensors with microfluidics-based biochemical analysis. In this study, a specific DNA-probe sequence and PCR primers were designed to be used in a magnetoresistive biosensing platform to amplify the internal transcribed spacer region of the ribosomal DNA of G. pallida. Magnetic nanoparticles were used as the labelling agents of asymmetric PCR product through biotin–streptavidin interaction. Upon target hybridization to sensor immobilized oligo probes, the fringe field created by the magnetic nanoparticles produces a variation in the sensor’s electrical resistance. The detection signal corresponds to the concentration of target molecules present in the sample. The results demonstrate the suitability of the magnetic biosensor to detect PCR target product and the specificity of the probe, which consistently distinguishes G. pallida (DV/V > 1%) from other cyst nematodes (DV/V < 1%), even when DNA mixtures were tested at different concentrations. This shows the magnetic biosensor’s potential as a bioanalytical device for field applications and border phytosanitary inspections.Bioresources 4 Sustainability (GREEN-IT)RUNCamacho, Maria JoãoAlbuquerque, Débora C.de Andrade, EugéniaMartins, Verónica C.Inácio, Maria L.Mota, ManuelFreitas, Paulo P.2023-09-29T22:21:29Z2023-012023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/158474eng1424-8220PURE: 72094680https://doi.org/10.3390/s23020647info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-18T01:43:37Zoai:run.unl.pt:10362/158474Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:09.442692Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A Lab-on-a-Chip Approach for the Detection of the Quarantine Potato Cyst Nematode Globodera pallida
title A Lab-on-a-Chip Approach for the Detection of the Quarantine Potato Cyst Nematode Globodera pallida
spellingShingle A Lab-on-a-Chip Approach for the Detection of the Quarantine Potato Cyst Nematode Globodera pallida
Camacho, Maria João
asymmetric PCR
magnetoresistive biochips
PCN
Analytical Chemistry
Information Systems
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering
title_short A Lab-on-a-Chip Approach for the Detection of the Quarantine Potato Cyst Nematode Globodera pallida
title_full A Lab-on-a-Chip Approach for the Detection of the Quarantine Potato Cyst Nematode Globodera pallida
title_fullStr A Lab-on-a-Chip Approach for the Detection of the Quarantine Potato Cyst Nematode Globodera pallida
title_full_unstemmed A Lab-on-a-Chip Approach for the Detection of the Quarantine Potato Cyst Nematode Globodera pallida
title_sort A Lab-on-a-Chip Approach for the Detection of the Quarantine Potato Cyst Nematode Globodera pallida
author Camacho, Maria João
author_facet Camacho, Maria João
Albuquerque, Débora C.
de Andrade, Eugénia
Martins, Verónica C.
Inácio, Maria L.
Mota, Manuel
Freitas, Paulo P.
author_role author
author2 Albuquerque, Débora C.
de Andrade, Eugénia
Martins, Verónica C.
Inácio, Maria L.
Mota, Manuel
Freitas, Paulo P.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Bioresources 4 Sustainability (GREEN-IT)
RUN
dc.contributor.author.fl_str_mv Camacho, Maria João
Albuquerque, Débora C.
de Andrade, Eugénia
Martins, Verónica C.
Inácio, Maria L.
Mota, Manuel
Freitas, Paulo P.
dc.subject.por.fl_str_mv asymmetric PCR
magnetoresistive biochips
PCN
Analytical Chemistry
Information Systems
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering
topic asymmetric PCR
magnetoresistive biochips
PCN
Analytical Chemistry
Information Systems
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering
description Funding Information: This research was funded by National Funds through the FCT—Foundation for Science and Technology under the Ph.D. fellowships BD 138724/2018, the project UIDB/05183/2020 (MED–Mediterranean Institute for Agriculture, Environment and Development, Institute for Advanced Studies and Research, Évora University) and the project UID/05367/2020 (PROGRAMATICO—Research Unit INESC MN, Microsystems and Nanotechnologies). Publisher Copyright: © 2023 by the authors.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-29T22:21:29Z
2023-01
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158474
url http://hdl.handle.net/10362/158474
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1424-8220
PURE: 72094680
https://doi.org/10.3390/s23020647
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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