Chondrogenic differentiation induced by extracellular vesicles bound to a nanofibrous substrate
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Publication Date: | 2021 |
Other Authors: | , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | https://hdl.handle.net/1822/78681 |
Summary: | Extracellular vesicles (EVs) are being increasingly studied owing to its regenerative potential, namely EVs derived from human bone marrow mesenchymal stem cells (hBM-MSCs). Those can be used for controlling inflammation, repairing injury, and enhancing tissue regeneration. Differently, the potential of EVs derived from human articular chondrocytes (hACs) to promote cartilage regeneration has not been thoroughly investigated. This work aims to develop an EVs immobilization system capable of selectively bind EVs present in conditioned medium obtained from cultures of hACs or hBM-MSC. For that, an anti-CD63 antibody was immobilized at the surface of an activated and functionalized electrospun nanofibrous mesh. The chondrogenic potential of bound EVs was further assessed by culturing hBM-MSCs during 28 days under basal conditions. EVs derived from hACs cultured under differentiation medium or from chondrogenically committed hBM-MSCs induced a chondrogenic phenotype characterized by marked induction of SOX9, COMP, Aggrecan and Collagen type II, and matrix glycosaminoglycans synthesis. Indeed, both EVs immobilization systems outperformed the currently used chondroinductive strategies. These data show that naturally secreted EVs can guide the chondrogenic commitment of hBM-MSCs in the absence of any other chemical or genetic chondrogenic inductors based in medium supplementation. |
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Chondrogenic differentiation induced by extracellular vesicles bound to a nanofibrous substrateBone marrow mesenchymal stem cellsChondrogenic differentiationElectrospun fibrous meshExtracellular vesiclesHuman articular chondrocytesScience & TechnologyExtracellular vesicles (EVs) are being increasingly studied owing to its regenerative potential, namely EVs derived from human bone marrow mesenchymal stem cells (hBM-MSCs). Those can be used for controlling inflammation, repairing injury, and enhancing tissue regeneration. Differently, the potential of EVs derived from human articular chondrocytes (hACs) to promote cartilage regeneration has not been thoroughly investigated. This work aims to develop an EVs immobilization system capable of selectively bind EVs present in conditioned medium obtained from cultures of hACs or hBM-MSC. For that, an anti-CD63 antibody was immobilized at the surface of an activated and functionalized electrospun nanofibrous mesh. The chondrogenic potential of bound EVs was further assessed by culturing hBM-MSCs during 28 days under basal conditions. EVs derived from hACs cultured under differentiation medium or from chondrogenically committed hBM-MSCs induced a chondrogenic phenotype characterized by marked induction of SOX9, COMP, Aggrecan and Collagen type II, and matrix glycosaminoglycans synthesis. Indeed, both EVs immobilization systems outperformed the currently used chondroinductive strategies. These data show that naturally secreted EVs can guide the chondrogenic commitment of hBM-MSCs in the absence of any other chemical or genetic chondrogenic inductors based in medium supplementation.The authors would like to acknowledge the Portuguese Foundation for Science and Technology (FCT) for the PhD grant to M.R.C. (PD/BD/113797/2015) financed by the Doctoral Program on Advanced Therapies for Health (PATH) (FSE/POCH/PD/169/2013) and the project Cells4_IDs (PTDC/BTM-SAL/28882/2017). This work was also financed by the Portuguese Mass Spectrometry Network, integrated in the National Roadmap of Research Infrastructures of Strategic Relevance (ROTEIRO/0028/2013; LISBOA-01-0145-FEDER-022125).Springer NatureUniversidade do MinhoCasanova, Marta Alexandra RodriguesOsório, HugoReis, R. L.Martins, AlbinoNeves, N. M.2021-112021-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/78681engCasanova M. R., Osório H., Reis R. L., Martins A., Neves N. M. Chondrogenic differentiation induced by extracellular vesicles bound to a nanofibrous substrate, npj Regenerative Medicine, Vol. 6, Issue 1, pp. 1 - 79, doi:10.1038/s41536-021-00190-8, 20212057-399510.1038/s41536-021-00190-8https://www.nature.com/articles/s41536-021-00190-8#citeasinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:55:48Zoai:repositorium.sdum.uminho.pt:1822/78681Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:45:21.608576Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Chondrogenic differentiation induced by extracellular vesicles bound to a nanofibrous substrate |
title |
Chondrogenic differentiation induced by extracellular vesicles bound to a nanofibrous substrate |
spellingShingle |
Chondrogenic differentiation induced by extracellular vesicles bound to a nanofibrous substrate Casanova, Marta Alexandra Rodrigues Bone marrow mesenchymal stem cells Chondrogenic differentiation Electrospun fibrous mesh Extracellular vesicles Human articular chondrocytes Science & Technology |
title_short |
Chondrogenic differentiation induced by extracellular vesicles bound to a nanofibrous substrate |
title_full |
Chondrogenic differentiation induced by extracellular vesicles bound to a nanofibrous substrate |
title_fullStr |
Chondrogenic differentiation induced by extracellular vesicles bound to a nanofibrous substrate |
title_full_unstemmed |
Chondrogenic differentiation induced by extracellular vesicles bound to a nanofibrous substrate |
title_sort |
Chondrogenic differentiation induced by extracellular vesicles bound to a nanofibrous substrate |
author |
Casanova, Marta Alexandra Rodrigues |
author_facet |
Casanova, Marta Alexandra Rodrigues Osório, Hugo Reis, R. L. Martins, Albino Neves, N. M. |
author_role |
author |
author2 |
Osório, Hugo Reis, R. L. Martins, Albino Neves, N. M. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Casanova, Marta Alexandra Rodrigues Osório, Hugo Reis, R. L. Martins, Albino Neves, N. M. |
dc.subject.por.fl_str_mv |
Bone marrow mesenchymal stem cells Chondrogenic differentiation Electrospun fibrous mesh Extracellular vesicles Human articular chondrocytes Science & Technology |
topic |
Bone marrow mesenchymal stem cells Chondrogenic differentiation Electrospun fibrous mesh Extracellular vesicles Human articular chondrocytes Science & Technology |
description |
Extracellular vesicles (EVs) are being increasingly studied owing to its regenerative potential, namely EVs derived from human bone marrow mesenchymal stem cells (hBM-MSCs). Those can be used for controlling inflammation, repairing injury, and enhancing tissue regeneration. Differently, the potential of EVs derived from human articular chondrocytes (hACs) to promote cartilage regeneration has not been thoroughly investigated. This work aims to develop an EVs immobilization system capable of selectively bind EVs present in conditioned medium obtained from cultures of hACs or hBM-MSC. For that, an anti-CD63 antibody was immobilized at the surface of an activated and functionalized electrospun nanofibrous mesh. The chondrogenic potential of bound EVs was further assessed by culturing hBM-MSCs during 28 days under basal conditions. EVs derived from hACs cultured under differentiation medium or from chondrogenically committed hBM-MSCs induced a chondrogenic phenotype characterized by marked induction of SOX9, COMP, Aggrecan and Collagen type II, and matrix glycosaminoglycans synthesis. Indeed, both EVs immobilization systems outperformed the currently used chondroinductive strategies. These data show that naturally secreted EVs can guide the chondrogenic commitment of hBM-MSCs in the absence of any other chemical or genetic chondrogenic inductors based in medium supplementation. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11 2021-11-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/78681 |
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https://hdl.handle.net/1822/78681 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Casanova M. R., Osório H., Reis R. L., Martins A., Neves N. M. Chondrogenic differentiation induced by extracellular vesicles bound to a nanofibrous substrate, npj Regenerative Medicine, Vol. 6, Issue 1, pp. 1 - 79, doi:10.1038/s41536-021-00190-8, 2021 2057-3995 10.1038/s41536-021-00190-8 https://www.nature.com/articles/s41536-021-00190-8#citeas |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Nature |
publisher.none.fl_str_mv |
Springer Nature |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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