Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.17533/udea.rccp.v34n3a06 http://hdl.handle.net/11449/233265 |
Resumo: | Background: Osteoarthritis is a complex degenerative disease with several factors contributing to joint damage. Objective: To compare the potential effect of hyaluronic acid (HA) and triamcinolone acetonide (TA), alone or combined, on the in vitro chondrogenic differentiation process of mesenchymal stem cells (MSCs). Methods: MSCs were divided into four groups: Control, HA, TA, and HA/TA combined. Each treatment group was cultured for 14 days in chondrogenic differentiation medium. The chondrogenic differentiation potential was assessed by histology and immunohistochemistry. Results: The HA and HA/TA-treated MSCs presented histological characteristics similar to native chondrocytes. The extracellular matrix (ECM) of TA-treated MSCs was compact and organized. Glycosaminoglycan staining was intense in Control, moderate in TA, slight in HA/TA, and undetectable in HA. Type II collagen immunoreactivity was high in the TA-treated ECM and MSCs. Conclusions: Histological analysis shows that HA influences morphological development similar to chondrocytes of the MSCs, but with low expression of specific cartilage molecules. The TA promotes formation of a compact and organized ECM. |
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Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cellsEfecto potencial del ácido hialurónico y del acetato de triamcinolona solos o combinados sobre la diferenciación condrogénica de células-tronco mesenquimalesEfeito potencial do ácido hialurônico e triancinolona acetonida, isolados e combinados, na diferenciação condrogênica de células-tronco mensenquimaisCartilageCell differentiationChondrocytesChondrogenesisChondrogenic differentiationCollagenCorticosteroidsGlucocorticoidHistologyHyaluronic acidMesenchymal stem cellOsteoarthritisTriancinolone acetonideViscosupplementationBackground: Osteoarthritis is a complex degenerative disease with several factors contributing to joint damage. Objective: To compare the potential effect of hyaluronic acid (HA) and triamcinolone acetonide (TA), alone or combined, on the in vitro chondrogenic differentiation process of mesenchymal stem cells (MSCs). Methods: MSCs were divided into four groups: Control, HA, TA, and HA/TA combined. Each treatment group was cultured for 14 days in chondrogenic differentiation medium. The chondrogenic differentiation potential was assessed by histology and immunohistochemistry. Results: The HA and HA/TA-treated MSCs presented histological characteristics similar to native chondrocytes. The extracellular matrix (ECM) of TA-treated MSCs was compact and organized. Glycosaminoglycan staining was intense in Control, moderate in TA, slight in HA/TA, and undetectable in HA. Type II collagen immunoreactivity was high in the TA-treated ECM and MSCs. Conclusions: Histological analysis shows that HA influences morphological development similar to chondrocytes of the MSCs, but with low expression of specific cartilage molecules. The TA promotes formation of a compact and organized ECM.São Paulo State University (Unesp) School of Veterinary Medicine and Animal ScienceFaculty of Veterinary Medicine Universidad Nacional de Colombia Research Group in Veterinary PathologySão Paulo State University (Unesp) School of Veterinary Medicine and Animal ScienceUniversidade Estadual Paulista (UNESP)Research Group in Veterinary PathologyOcampo, Pablo E. [UNESP]Vallejo, Viviana [UNESP]Montoya, Luis M.Rocha, Noeme S. [UNESP]Landim, Fernanda da C. [UNESP]Rahal, Sheila C. [UNESP]2022-05-01T06:02:33Z2022-05-01T06:02:33Z2021-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article212-223http://dx.doi.org/10.17533/udea.rccp.v34n3a06Revista Colombiana de Ciencias Pecuarias, v. 34, n. 3, p. 212-223, 2021.2256-29580120-0690http://hdl.handle.net/11449/23326510.17533/udea.rccp.v34n3a062-s2.0-85110174025Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Colombiana de Ciencias Pecuariasinfo:eu-repo/semantics/openAccess2022-05-01T06:02:33Zoai:repositorio.unesp.br:11449/233265Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-05-01T06:02:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells Efecto potencial del ácido hialurónico y del acetato de triamcinolona solos o combinados sobre la diferenciación condrogénica de células-tronco mesenquimales Efeito potencial do ácido hialurônico e triancinolona acetonida, isolados e combinados, na diferenciação condrogênica de células-tronco mensenquimais |
title |
Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells |
spellingShingle |
Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells Ocampo, Pablo E. [UNESP] Cartilage Cell differentiation Chondrocytes Chondrogenesis Chondrogenic differentiation Collagen Corticosteroids Glucocorticoid Histology Hyaluronic acid Mesenchymal stem cell Osteoarthritis Triancinolone acetonide Viscosupplementation |
title_short |
Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells |
title_full |
Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells |
title_fullStr |
Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells |
title_full_unstemmed |
Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells |
title_sort |
Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells |
author |
Ocampo, Pablo E. [UNESP] |
author_facet |
Ocampo, Pablo E. [UNESP] Vallejo, Viviana [UNESP] Montoya, Luis M. Rocha, Noeme S. [UNESP] Landim, Fernanda da C. [UNESP] Rahal, Sheila C. [UNESP] |
author_role |
author |
author2 |
Vallejo, Viviana [UNESP] Montoya, Luis M. Rocha, Noeme S. [UNESP] Landim, Fernanda da C. [UNESP] Rahal, Sheila C. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Research Group in Veterinary Pathology |
dc.contributor.author.fl_str_mv |
Ocampo, Pablo E. [UNESP] Vallejo, Viviana [UNESP] Montoya, Luis M. Rocha, Noeme S. [UNESP] Landim, Fernanda da C. [UNESP] Rahal, Sheila C. [UNESP] |
dc.subject.por.fl_str_mv |
Cartilage Cell differentiation Chondrocytes Chondrogenesis Chondrogenic differentiation Collagen Corticosteroids Glucocorticoid Histology Hyaluronic acid Mesenchymal stem cell Osteoarthritis Triancinolone acetonide Viscosupplementation |
topic |
Cartilage Cell differentiation Chondrocytes Chondrogenesis Chondrogenic differentiation Collagen Corticosteroids Glucocorticoid Histology Hyaluronic acid Mesenchymal stem cell Osteoarthritis Triancinolone acetonide Viscosupplementation |
description |
Background: Osteoarthritis is a complex degenerative disease with several factors contributing to joint damage. Objective: To compare the potential effect of hyaluronic acid (HA) and triamcinolone acetonide (TA), alone or combined, on the in vitro chondrogenic differentiation process of mesenchymal stem cells (MSCs). Methods: MSCs were divided into four groups: Control, HA, TA, and HA/TA combined. Each treatment group was cultured for 14 days in chondrogenic differentiation medium. The chondrogenic differentiation potential was assessed by histology and immunohistochemistry. Results: The HA and HA/TA-treated MSCs presented histological characteristics similar to native chondrocytes. The extracellular matrix (ECM) of TA-treated MSCs was compact and organized. Glycosaminoglycan staining was intense in Control, moderate in TA, slight in HA/TA, and undetectable in HA. Type II collagen immunoreactivity was high in the TA-treated ECM and MSCs. Conclusions: Histological analysis shows that HA influences morphological development similar to chondrocytes of the MSCs, but with low expression of specific cartilage molecules. The TA promotes formation of a compact and organized ECM. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-01 2022-05-01T06:02:33Z 2022-05-01T06:02:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.17533/udea.rccp.v34n3a06 Revista Colombiana de Ciencias Pecuarias, v. 34, n. 3, p. 212-223, 2021. 2256-2958 0120-0690 http://hdl.handle.net/11449/233265 10.17533/udea.rccp.v34n3a06 2-s2.0-85110174025 |
url |
http://dx.doi.org/10.17533/udea.rccp.v34n3a06 http://hdl.handle.net/11449/233265 |
identifier_str_mv |
Revista Colombiana de Ciencias Pecuarias, v. 34, n. 3, p. 212-223, 2021. 2256-2958 0120-0690 10.17533/udea.rccp.v34n3a06 2-s2.0-85110174025 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Colombiana de Ciencias Pecuarias |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
212-223 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964871596441600 |