Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells

• Autor(a) principal: Ocampo, Pablo E. [UNESP]
• Data de Publicação: 2021
• Outros Autores: Vallejo, Viviana [UNESP], Montoya, Luis M., Rocha, Noeme S. [UNESP], Landim, Fernanda da C. [UNESP], Rahal, Sheila C. [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.17533/udea.rccp.v34n3a06; http://hdl.handle.net/11449/233265
• topic: Cartilage; Cell differentiation; Chondrocytes; Chondrogenesis; Chondrogenic differentiation; Collagen; Corticosteroids; Glucocorticoid; Histology; Hyaluronic acid; Mesenchymal stem cell; Osteoarthritis; Triancinolone acetonide; Viscosupplementation

Background: Osteoarthritis is a complex degenerative disease with several factors contributing to joint damage. Objective: To compare the potential effect of hyaluronic acid (HA) and triamcinolone acetonide (TA), alone or combined, on the in vitro chondrogenic differentiation process of mesenchymal stem cells (MSCs). Methods: MSCs were divided into four groups: Control, HA, TA, and HA/TA combined. Each treatment group was cultured for 14 days in chondrogenic differentiation medium. The chondrogenic differentiation potential was assessed by histology and immunohistochemistry. Results: The HA and HA/TA-treated MSCs presented histological characteristics similar to native chondrocytes. The extracellular matrix (ECM) of TA-treated MSCs was compact and organized. Glycosaminoglycan staining was intense in Control, moderate in TA, slight in HA/TA, and undetectable in HA. Type II collagen immunoreactivity was high in the TA-treated ECM and MSCs. Conclusions: Histological analysis shows that HA influences morphological development similar to chondrocytes of the MSCs, but with low expression of specific cartilage molecules. The TA promotes formation of a compact and organized ECM.