Implementation of Novel Affinity Ligand for Lentiviral Vector Purification

Detalhes bibliográficos
Autor(a) principal: Moreira, Ana Sofia
Data de Publicação: 2023
Outros Autores: Bezemer, Sandra, Faria, Tiago Q., Detmers, Frank, Hermans, Pim, Sierkstra, Laurens, Coroadinha, Ana Sofia, Peixoto, Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158537
Resumo: Funding Information: The authors would like to acknowledge funding from Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020) and the Associate Laboratory LS4FUTURE (LA/P/0087/2020). The author Ana Sofia Moreira acknowledges FCT/MCES for the PhD fellowship PD/BD/135501/2018. Publisher Copyright: © 2023 by the authors.
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spelling Implementation of Novel Affinity Ligand for Lentiviral Vector Purificationaffinity chromatographycell therapygene therapylentiviral vectorviral vector manufacturingCatalysisMolecular BiologySpectroscopyComputer Science ApplicationsPhysical and Theoretical ChemistryOrganic ChemistryInorganic ChemistrySDG 3 - Good Health and Well-beingFunding Information: The authors would like to acknowledge funding from Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020) and the Associate Laboratory LS4FUTURE (LA/P/0087/2020). The author Ana Sofia Moreira acknowledges FCT/MCES for the PhD fellowship PD/BD/135501/2018. Publisher Copyright: © 2023 by the authors.The use of viral vectors as therapeutic products for multiple applications such as vaccines, cancer treatment, or gene therapies, has been growing exponentially. Therefore, improved manufacturing processes are needed to cope with the high number of functional particles required for clinical trials and, eventually, commercialization. Affinity chromatography (AC) can be used to simplify purification processes and generate clinical-grade products with high titer and purity. However, one of the major challenges in the purification of Lentiviral vectors (LVs) using AC is to combine a highly specific ligand with a gentle elution condition assuring the preservation of vector biological activity. In this work, we report for the first time the implementation of an AC resin to specifically purify VSV-G pseudotyped LVs. After ligand screening, different critical process parameters were assessed and optimized. A dynamic capacity of 1 × 1011 total particles per mL of resin was determined and an average recovery yield of 45% was found for the small-scale purification process. The established AC robustness was confirmed by the performance of an intermediate scale providing an infectious particles yield of 54%, which demonstrates the scalability and reproducibility of the AC matrix. Overall, this work contributes to increasing downstream process efficiency by delivering a purification technology that enables high purity, scalability, and process intensification in a single step, contributing to time-to-market reduction.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNMoreira, Ana SofiaBezemer, SandraFaria, Tiago Q.Detmers, FrankHermans, PimSierkstra, LaurensCoroadinha, Ana SofiaPeixoto, Cristina2023-09-30T22:20:56Z2023-022023-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/158537eng1661-6596PURE: 72614462https://doi.org/10.3390/ijms24043354info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:41:05Zoai:run.unl.pt:10362/158537Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:11.920274Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Implementation of Novel Affinity Ligand for Lentiviral Vector Purification
title Implementation of Novel Affinity Ligand for Lentiviral Vector Purification
spellingShingle Implementation of Novel Affinity Ligand for Lentiviral Vector Purification
Moreira, Ana Sofia
affinity chromatography
cell therapy
gene therapy
lentiviral vector
viral vector manufacturing
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
SDG 3 - Good Health and Well-being
title_short Implementation of Novel Affinity Ligand for Lentiviral Vector Purification
title_full Implementation of Novel Affinity Ligand for Lentiviral Vector Purification
title_fullStr Implementation of Novel Affinity Ligand for Lentiviral Vector Purification
title_full_unstemmed Implementation of Novel Affinity Ligand for Lentiviral Vector Purification
title_sort Implementation of Novel Affinity Ligand for Lentiviral Vector Purification
author Moreira, Ana Sofia
author_facet Moreira, Ana Sofia
Bezemer, Sandra
Faria, Tiago Q.
Detmers, Frank
Hermans, Pim
Sierkstra, Laurens
Coroadinha, Ana Sofia
Peixoto, Cristina
author_role author
author2 Bezemer, Sandra
Faria, Tiago Q.
Detmers, Frank
Hermans, Pim
Sierkstra, Laurens
Coroadinha, Ana Sofia
Peixoto, Cristina
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Moreira, Ana Sofia
Bezemer, Sandra
Faria, Tiago Q.
Detmers, Frank
Hermans, Pim
Sierkstra, Laurens
Coroadinha, Ana Sofia
Peixoto, Cristina
dc.subject.por.fl_str_mv affinity chromatography
cell therapy
gene therapy
lentiviral vector
viral vector manufacturing
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
SDG 3 - Good Health and Well-being
topic affinity chromatography
cell therapy
gene therapy
lentiviral vector
viral vector manufacturing
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
SDG 3 - Good Health and Well-being
description Funding Information: The authors would like to acknowledge funding from Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020) and the Associate Laboratory LS4FUTURE (LA/P/0087/2020). The author Ana Sofia Moreira acknowledges FCT/MCES for the PhD fellowship PD/BD/135501/2018. Publisher Copyright: © 2023 by the authors.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-30T22:20:56Z
2023-02
2023-02-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158537
url http://hdl.handle.net/10362/158537
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1661-6596
PURE: 72614462
https://doi.org/10.3390/ijms24043354
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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