Establishment of Murine Gammaherpesvirus latency in B cells is not a stochastic event

Detalhes bibliográficos
Autor(a) principal: Decalf, Jérémie
Data de Publicação: 2014
Outros Autores: Godinho-Silva, Cristina, Fontinha, Diana, Marques, Sofia, Simas, J Pedro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/49889
Resumo: Copyright: © 2014 Decalf et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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spelling Establishment of Murine Gammaherpesvirus latency in B cells is not a stochastic eventCopyright: © 2014 Decalf et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Murid γ-herpesvirus-4 (MuHV-4) promotes polyclonal B cell activation and establishes latency in memory B cells via unclear mechanisms. We aimed at exploring whether B cell receptor specificity plays a role in B cell susceptibility to viral latency and how this is related to B cell activation. We first observed that MuHV-4-specific B cells represent a minority of the latent population, and to better understand the influence of the virus on non-MuHV-4 specific B cells we used the SWHEL mouse model, which produce hen egg lysozyme (HEL)-specific B cells. By tracking HEL+ and HEL- B cells, we showed that in vivo latency was restricted to HEL- B cells while the two populations were equally sensitive to the virus in vitro. Moreover, MuHV-4 induced two waves of B cell activation. While the first wave was characterized by a general B cell activation, as shown by HEL+ and HEL- B cells expansion and upregulation of CD69 expression, the second wave was restricted to the HEL- population, which acquired germinal center (GC) and plasma cell phenotypes. Antigenic stimulation of HEL+ B cells led to the development of HEL+ GC B cells where latent infection remained undetectable, indicating that MuHV-4 does not benefit from acute B cell responses to establish latency in non-virus specific B cells but relies on other mechanisms of the humoral response. These data support a model in which the establishment of latency in B cells by γ-herpesviruses is not stochastic in terms of BCR specificity and is tightly linked to the formation of GCs.Research in JPS's lab was supported by a grant from the Portuguese Fundação para a Ciência e Tecnologia (FCT) (PTDC/SAU-MII/099314/2008). JD was recipient of a FCT fellowship.PLOSRepositório da Universidade de LisboaDecalf, JérémieGodinho-Silva, CristinaFontinha, DianaMarques, SofiaSimas, J Pedro2021-10-14T13:12:28Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/49889engPLoS Pathog. 2014 Jul 31;10(7):e10042691553-736610.1371/journal.ppat.10042691553-7374info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:53:51Zoai:repositorio.ul.pt:10451/49889Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:01:25.285006Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Establishment of Murine Gammaherpesvirus latency in B cells is not a stochastic event
title Establishment of Murine Gammaherpesvirus latency in B cells is not a stochastic event
spellingShingle Establishment of Murine Gammaherpesvirus latency in B cells is not a stochastic event
Decalf, Jérémie
title_short Establishment of Murine Gammaherpesvirus latency in B cells is not a stochastic event
title_full Establishment of Murine Gammaherpesvirus latency in B cells is not a stochastic event
title_fullStr Establishment of Murine Gammaherpesvirus latency in B cells is not a stochastic event
title_full_unstemmed Establishment of Murine Gammaherpesvirus latency in B cells is not a stochastic event
title_sort Establishment of Murine Gammaherpesvirus latency in B cells is not a stochastic event
author Decalf, Jérémie
author_facet Decalf, Jérémie
Godinho-Silva, Cristina
Fontinha, Diana
Marques, Sofia
Simas, J Pedro
author_role author
author2 Godinho-Silva, Cristina
Fontinha, Diana
Marques, Sofia
Simas, J Pedro
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Decalf, Jérémie
Godinho-Silva, Cristina
Fontinha, Diana
Marques, Sofia
Simas, J Pedro
description Copyright: © 2014 Decalf et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
2021-10-14T13:12:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/49889
url http://hdl.handle.net/10451/49889
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PLoS Pathog. 2014 Jul 31;10(7):e1004269
1553-7366
10.1371/journal.ppat.1004269
1553-7374
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PLOS
publisher.none.fl_str_mv PLOS
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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