Murid Gammaherpesvirus latency-associated protein M2 promotes the formation of conjugates between transformed B lymphoma cells and T helper cells

Detalhes bibliográficos
Autor(a) principal: Fontinha, Diana
Data de Publicação: 2015
Outros Autores: Lopes, Filipa B., Marques, Sofia, Simas, J Pedro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/51153
Resumo: Copyright: © 2015 Fontinha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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spelling Murid Gammaherpesvirus latency-associated protein M2 promotes the formation of conjugates between transformed B lymphoma cells and T helper cellsCopyright: © 2015 Fontinha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedEstablishment of persistent infection in memory B cells by murid herpesvirus-4 (MuHV-4) depends on the proliferation of latently infected germinal center B cells, for which T cell help is essential. Whether the virus is capable of modulating B-T helper cell interaction for its own benefit is still unknown. Here, we investigate if the MuHV-4 latency associated M2 protein, which assembles multiprotein complexes with B cell signaling proteins, plays a role. We observed that M2 led to the upregulation of adhesion and co-stimulatory molecules in transduced B cell lines. In an MHC-II restricted OVA peptide-specific system, M2 polarized to the B-T helper contact zone. Furthermore, it promoted B cell polarization, as demonstrated by the increased proximity of the B cell microtubule organizing center to the interface. Consistent with these data, M2 promoted the formation of B-T helper cell conjugates. In an in vitro competition assay, this translated into a competitive advantage, as T cells preferentially conjugated with M2-expressing B cells. However, expression of M2 alone in B cells was not sufficient to lead to T cell activation, as it only occurred in the presence of specific peptide. Taken together, these findings support that M2 promotes the formation of B-T helper cell conjugates. In an in vivo context this may confer a competitive advantage to the infected B cell in acquisition of T cell help and initiation of a germinal center reaction, hence host colonization.The authors received funding for this work from Fundação para a Ciência e a Tecnologia (PTDC/SAU-MII/099314/2008).PLOSRepositório da Universidade de LisboaFontinha, DianaLopes, Filipa B.Marques, SofiaSimas, J Pedro2022-02-07T15:44:28Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/51153engPLoS One. 2015 Nov 6;10(11):e014254010.1371/journal.pone.01425401932-6203info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:55:39Zoai:repositorio.ul.pt:10451/51153Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:02:27.484430Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Murid Gammaherpesvirus latency-associated protein M2 promotes the formation of conjugates between transformed B lymphoma cells and T helper cells
title Murid Gammaherpesvirus latency-associated protein M2 promotes the formation of conjugates between transformed B lymphoma cells and T helper cells
spellingShingle Murid Gammaherpesvirus latency-associated protein M2 promotes the formation of conjugates between transformed B lymphoma cells and T helper cells
Fontinha, Diana
title_short Murid Gammaherpesvirus latency-associated protein M2 promotes the formation of conjugates between transformed B lymphoma cells and T helper cells
title_full Murid Gammaherpesvirus latency-associated protein M2 promotes the formation of conjugates between transformed B lymphoma cells and T helper cells
title_fullStr Murid Gammaherpesvirus latency-associated protein M2 promotes the formation of conjugates between transformed B lymphoma cells and T helper cells
title_full_unstemmed Murid Gammaherpesvirus latency-associated protein M2 promotes the formation of conjugates between transformed B lymphoma cells and T helper cells
title_sort Murid Gammaherpesvirus latency-associated protein M2 promotes the formation of conjugates between transformed B lymphoma cells and T helper cells
author Fontinha, Diana
author_facet Fontinha, Diana
Lopes, Filipa B.
Marques, Sofia
Simas, J Pedro
author_role author
author2 Lopes, Filipa B.
Marques, Sofia
Simas, J Pedro
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Fontinha, Diana
Lopes, Filipa B.
Marques, Sofia
Simas, J Pedro
description Copyright: © 2015 Fontinha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2022-02-07T15:44:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/51153
url http://hdl.handle.net/10451/51153
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PLoS One. 2015 Nov 6;10(11):e0142540
10.1371/journal.pone.0142540
1932-6203
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PLOS
publisher.none.fl_str_mv PLOS
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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