Structural Characterization of N-Linked Glycans in the Receptor Binding Domain of the SARS-CoV-2 Spike Protein and their Interactions with Human Lectins

Detalhes bibliográficos
Autor(a) principal: Lenza, Maria Pia
Data de Publicação: 2020
Outros Autores: Oyenarte, Iker, Diercks, Tammo, Quintana, Jon Imanol, Gimeno, Ana, Coelho, Helena, Diniz, Ana, Peccati, Francesca, Delgado, Sandra, Bosch, Alexandre, Valle, Mikel, Millet, Oscar, Abrescia, Nicola G. A., Palazón, Asís, Marcelo, Filipa, Jiménez-Osés, Gonzalo, Jiménez-Barbero, Jesús, Ardá, Ana, Ereño-Orbea, June
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/117141
Resumo: info:eu-repo/grantAgreement/WT/Physiological Sciences/095700 ERC‐2017‐AdG, 788143‐RECGLYCANMR grant 200077 grant RTI2018‐094751‐B‐C21 GC2018‐098996‐B‐I00 RTI2018‐099592‐B‐C22 RTI2018‐101269‐B‐I00 SEV‐2016‐0644 IF/00780/2015 PTDC/BIA‐MIB/31028/2017 UCIBIO UIDB/04378/2020 Infrastructure project 22161 PD/BD/142847/2018
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network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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spelling Structural Characterization of N-Linked Glycans in the Receptor Binding Domain of the SARS-CoV-2 Spike Protein and their Interactions with Human Lectinsglycanlectinmolecular recognitionreceptor binding domainSARS-CoV2CatalysisChemistry(all)info:eu-repo/grantAgreement/WT/Physiological Sciences/095700 ERC‐2017‐AdG, 788143‐RECGLYCANMR grant 200077 grant RTI2018‐094751‐B‐C21 GC2018‐098996‐B‐I00 RTI2018‐099592‐B‐C22 RTI2018‐101269‐B‐I00 SEV‐2016‐0644 IF/00780/2015 PTDC/BIA‐MIB/31028/2017 UCIBIO UIDB/04378/2020 Infrastructure project 22161 PD/BD/142847/2018The glycan structures of the receptor binding domain of the SARS-CoV2 spike glycoprotein expressed in human HEK293F cells have been studied by using NMR. The different possible interacting epitopes have been deeply analysed and characterized, providing evidence of the presence of glycan structures not found in previous MS-based analyses. The interaction of the RBD 13C-labelled glycans with different human lectins, which are expressed in different organs and tissues that may be affected during the infection process, has also been evaluated by NMR. In particular, 15N-labelled galectins (galectins-3, -7 and -8 N-terminal), Siglecs (Siglec-8, Siglec-10), and C-type lectins (DC-SIGN, MGL) have been employed. Complementary experiments from the glycoprotein perspective or from the lectin's point of view have permitted to disentangle the specific interacting epitopes in each case. Based on these findings, 3D models of the interacting complexes have been proposed.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNLenza, Maria PiaOyenarte, IkerDiercks, TammoQuintana, Jon ImanolGimeno, AnaCoelho, HelenaDiniz, AnaPeccati, FrancescaDelgado, SandraBosch, AlexandreValle, MikelMillet, OscarAbrescia, Nicola G. A.Palazón, AsísMarcelo, FilipaJiménez-Osés, GonzaloJiménez-Barbero, JesúsArdá, AnaEreño-Orbea, June2021-05-05T23:25:28Z2020-12-212020-12-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/117141eng1433-7851PURE: 26745157https://doi.org/10.1002/anie.202011015info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:00:11Zoai:run.unl.pt:10362/117141Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:29.701983Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Structural Characterization of N-Linked Glycans in the Receptor Binding Domain of the SARS-CoV-2 Spike Protein and their Interactions with Human Lectins
title Structural Characterization of N-Linked Glycans in the Receptor Binding Domain of the SARS-CoV-2 Spike Protein and their Interactions with Human Lectins
spellingShingle Structural Characterization of N-Linked Glycans in the Receptor Binding Domain of the SARS-CoV-2 Spike Protein and their Interactions with Human Lectins
Lenza, Maria Pia
glycan
lectin
molecular recognition
receptor binding domain
SARS-CoV2
Catalysis
Chemistry(all)
title_short Structural Characterization of N-Linked Glycans in the Receptor Binding Domain of the SARS-CoV-2 Spike Protein and their Interactions with Human Lectins
title_full Structural Characterization of N-Linked Glycans in the Receptor Binding Domain of the SARS-CoV-2 Spike Protein and their Interactions with Human Lectins
title_fullStr Structural Characterization of N-Linked Glycans in the Receptor Binding Domain of the SARS-CoV-2 Spike Protein and their Interactions with Human Lectins
title_full_unstemmed Structural Characterization of N-Linked Glycans in the Receptor Binding Domain of the SARS-CoV-2 Spike Protein and their Interactions with Human Lectins
title_sort Structural Characterization of N-Linked Glycans in the Receptor Binding Domain of the SARS-CoV-2 Spike Protein and their Interactions with Human Lectins
author Lenza, Maria Pia
author_facet Lenza, Maria Pia
Oyenarte, Iker
Diercks, Tammo
Quintana, Jon Imanol
Gimeno, Ana
Coelho, Helena
Diniz, Ana
Peccati, Francesca
Delgado, Sandra
Bosch, Alexandre
Valle, Mikel
Millet, Oscar
Abrescia, Nicola G. A.
Palazón, Asís
Marcelo, Filipa
Jiménez-Osés, Gonzalo
Jiménez-Barbero, Jesús
Ardá, Ana
Ereño-Orbea, June
author_role author
author2 Oyenarte, Iker
Diercks, Tammo
Quintana, Jon Imanol
Gimeno, Ana
Coelho, Helena
Diniz, Ana
Peccati, Francesca
Delgado, Sandra
Bosch, Alexandre
Valle, Mikel
Millet, Oscar
Abrescia, Nicola G. A.
Palazón, Asís
Marcelo, Filipa
Jiménez-Osés, Gonzalo
Jiménez-Barbero, Jesús
Ardá, Ana
Ereño-Orbea, June
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Lenza, Maria Pia
Oyenarte, Iker
Diercks, Tammo
Quintana, Jon Imanol
Gimeno, Ana
Coelho, Helena
Diniz, Ana
Peccati, Francesca
Delgado, Sandra
Bosch, Alexandre
Valle, Mikel
Millet, Oscar
Abrescia, Nicola G. A.
Palazón, Asís
Marcelo, Filipa
Jiménez-Osés, Gonzalo
Jiménez-Barbero, Jesús
Ardá, Ana
Ereño-Orbea, June
dc.subject.por.fl_str_mv glycan
lectin
molecular recognition
receptor binding domain
SARS-CoV2
Catalysis
Chemistry(all)
topic glycan
lectin
molecular recognition
receptor binding domain
SARS-CoV2
Catalysis
Chemistry(all)
description info:eu-repo/grantAgreement/WT/Physiological Sciences/095700 ERC‐2017‐AdG, 788143‐RECGLYCANMR grant 200077 grant RTI2018‐094751‐B‐C21 GC2018‐098996‐B‐I00 RTI2018‐099592‐B‐C22 RTI2018‐101269‐B‐I00 SEV‐2016‐0644 IF/00780/2015 PTDC/BIA‐MIB/31028/2017 UCIBIO UIDB/04378/2020 Infrastructure project 22161 PD/BD/142847/2018
publishDate 2020
dc.date.none.fl_str_mv 2020-12-21
2020-12-21T00:00:00Z
2021-05-05T23:25:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/117141
url http://hdl.handle.net/10362/117141
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1433-7851
PURE: 26745157
https://doi.org/10.1002/anie.202011015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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