Laccase-mediator system for the ionic liquid-assisted treatment of a technical lignin with partial dissolution

Detalhes bibliográficos
Autor(a) principal: Pena, Carlos A.
Data de Publicação: 2023
Outros Autores: Ballesteros, Lina F., Rodríguez, Héctor, Rodil, Eva, Teixeira, J. A., Michelin, Michele
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/86401
Resumo: The valorisation of lignin is a key aspect in the optimal exploitation of the paradigm of a biorefinery based on lignocellulosic biomass. In this work, the treatment of the technical lignin Indulin AT with a recombinant laccase, or with a combination of this enzyme with the mediator 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), has been carried out at 25 °C in a mildly acidic buffered aqueous medium. The presence of the ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) has also been investigated. Due to the limited solubility of Indulin AT in water, only partial dissolution occurred. In the lignin recovered after the treatments, a preferential diminution of the guaiacyl units was observed. In the analysis of the aqueous phases, a variety of phenolic compounds were identified, deriving from either guaiacyl or p-hydroxyphenyl units, although in moderately low concentrations. The highest assigned concentrations, always achieved when using the laccase-ABTS system (versus the treatment with no mediator), corresponded to different phenolic compounds, depending on whether the ionic liquid was involved or not in the treatment. In general, the overall concentration of phenolic compounds (measured as equivalents of gallic acid) was much greater after treatments involving the ionic liquid.
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spelling Laccase-mediator system for the ionic liquid-assisted treatment of a technical lignin with partial dissolutionLigninLaccaseIonic liquidLaccase-mediator systemPhenolic compoundsThe valorisation of lignin is a key aspect in the optimal exploitation of the paradigm of a biorefinery based on lignocellulosic biomass. In this work, the treatment of the technical lignin Indulin AT with a recombinant laccase, or with a combination of this enzyme with the mediator 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), has been carried out at 25 °C in a mildly acidic buffered aqueous medium. The presence of the ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) has also been investigated. Due to the limited solubility of Indulin AT in water, only partial dissolution occurred. In the lignin recovered after the treatments, a preferential diminution of the guaiacyl units was observed. In the analysis of the aqueous phases, a variety of phenolic compounds were identified, deriving from either guaiacyl or p-hydroxyphenyl units, although in moderately low concentrations. The highest assigned concentrations, always achieved when using the laccase-ABTS system (versus the treatment with no mediator), corresponded to different phenolic compounds, depending on whether the ionic liquid was involved or not in the treatment. In general, the overall concentration of phenolic compounds (measured as equivalents of gallic acid) was much greater after treatments involving the ionic liquid.This work was supported by Xunta de Galicia through grant numbers ED431B 2017/023 and ED431E 2018/01 (CRETUS Strategic Partnership), and by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and by LABBELS – Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020. C.A.P. and H.R. are grateful to “Agrupación Europea de Cooperación Territorial – Galicia - Norte de Portugal (GNP-AECT)” for support of stays at the Centre of Biological Engineering of the University of Minho via the Iacobus programme. The use of RIAIDT-USC analytical facilities is also acknowledged.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoPena, Carlos A.Ballesteros, Lina F.Rodríguez, HéctorRodil, EvaTeixeira, J. A.Michelin, Michele2023-102023-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/86401engPena, C. A., Ballesteros, L. F., Rodríguez, H., Rodil, E., Teixeira, J. A., & Michelin, M. (2023, October). Laccase-mediator system for the ionic liquid-assisted treatment of a technical lignin with partial dissolution. Biomass and Bioenergy. Elsevier BV. http://doi.org/10.1016/j.biombioe.2023.1069280961-953410.1016/j.biombioe.2023.106928106928https://www.sciencedirect.com/science/article/pii/S0961953423002271info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-09-16T01:17:09Zoai:repositorium.sdum.uminho.pt:1822/86401Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:29:22.334112Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Laccase-mediator system for the ionic liquid-assisted treatment of a technical lignin with partial dissolution
title Laccase-mediator system for the ionic liquid-assisted treatment of a technical lignin with partial dissolution
spellingShingle Laccase-mediator system for the ionic liquid-assisted treatment of a technical lignin with partial dissolution
Pena, Carlos A.
Lignin
Laccase
Ionic liquid
Laccase-mediator system
Phenolic compounds
title_short Laccase-mediator system for the ionic liquid-assisted treatment of a technical lignin with partial dissolution
title_full Laccase-mediator system for the ionic liquid-assisted treatment of a technical lignin with partial dissolution
title_fullStr Laccase-mediator system for the ionic liquid-assisted treatment of a technical lignin with partial dissolution
title_full_unstemmed Laccase-mediator system for the ionic liquid-assisted treatment of a technical lignin with partial dissolution
title_sort Laccase-mediator system for the ionic liquid-assisted treatment of a technical lignin with partial dissolution
author Pena, Carlos A.
author_facet Pena, Carlos A.
Ballesteros, Lina F.
Rodríguez, Héctor
Rodil, Eva
Teixeira, J. A.
Michelin, Michele
author_role author
author2 Ballesteros, Lina F.
Rodríguez, Héctor
Rodil, Eva
Teixeira, J. A.
Michelin, Michele
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pena, Carlos A.
Ballesteros, Lina F.
Rodríguez, Héctor
Rodil, Eva
Teixeira, J. A.
Michelin, Michele
dc.subject.por.fl_str_mv Lignin
Laccase
Ionic liquid
Laccase-mediator system
Phenolic compounds
topic Lignin
Laccase
Ionic liquid
Laccase-mediator system
Phenolic compounds
description The valorisation of lignin is a key aspect in the optimal exploitation of the paradigm of a biorefinery based on lignocellulosic biomass. In this work, the treatment of the technical lignin Indulin AT with a recombinant laccase, or with a combination of this enzyme with the mediator 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), has been carried out at 25 °C in a mildly acidic buffered aqueous medium. The presence of the ionic liquid 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) has also been investigated. Due to the limited solubility of Indulin AT in water, only partial dissolution occurred. In the lignin recovered after the treatments, a preferential diminution of the guaiacyl units was observed. In the analysis of the aqueous phases, a variety of phenolic compounds were identified, deriving from either guaiacyl or p-hydroxyphenyl units, although in moderately low concentrations. The highest assigned concentrations, always achieved when using the laccase-ABTS system (versus the treatment with no mediator), corresponded to different phenolic compounds, depending on whether the ionic liquid was involved or not in the treatment. In general, the overall concentration of phenolic compounds (measured as equivalents of gallic acid) was much greater after treatments involving the ionic liquid.
publishDate 2023
dc.date.none.fl_str_mv 2023-10
2023-10-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/86401
url https://hdl.handle.net/1822/86401
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pena, C. A., Ballesteros, L. F., Rodríguez, H., Rodil, E., Teixeira, J. A., & Michelin, M. (2023, October). Laccase-mediator system for the ionic liquid-assisted treatment of a technical lignin with partial dissolution. Biomass and Bioenergy. Elsevier BV. http://doi.org/10.1016/j.biombioe.2023.106928
0961-9534
10.1016/j.biombioe.2023.106928
106928
https://www.sciencedirect.com/science/article/pii/S0961953423002271
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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