Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine Endometrium

Detalhes bibliográficos
Autor(a) principal: Amaral, Ana
Data de Publicação: 2023
Outros Autores: Cebola, Nélio, Zóstek-Mioduchowska, Anna, Rebordão, Maria Rosa, Kordowitzki, Paweł, Skarzynski, Dariusz, Ferreira-Dias, Graça
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/27793
Resumo: Research Areas: Biochemistry & Molecular Biology ; Chemistry
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spelling Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine EndometriumEquineendometrosisfibrosismyeloperoxidasenoscapineinhibitioncollagenResearch Areas: Biochemistry & Molecular Biology ; ChemistryMyeloperoxidase is an enzyme released by neutrophils when neutrophil extracellular traps (NETs) are formed. Besides myeloperoxidase activity against pathogens, it was also linked to many diseases, including inflammatory and fibrotic ones. Endometrosis is a fibrotic disease of the mare endometrium, with a large impact on their fertility, where myeloperoxidase was shown to induce fibrosis. Noscapine is an alkaloid with a low toxicity, that has been studied as an anti-cancer drug, and more recently as an anti-fibrotic molecule. This work aims to evaluate noscapine inhibition of collagen type 1 (COL1) induced by myeloperoxidase in equine endometrial explants from follicular and mid-luteal phases, at 24 and 48 h of treatment. The transcription of collagen type 1 alpha 2 chain (COL1A2), and COL1 protein relative abundance were evaluated by qPCR and Western blot, respectively. The treatment with myeloperoxidase increased COL1A2 mRNA transcription and COL1 protein, whereas noscapine was able to reduce this effect with respect to COL1A2 mRNA transcription, in a time/estrous cycle phase-dependent manner (in explants from the follicular phase, at 24 h of treatment). Our study indicates that noscapine is a promising drug to be considered as an anti-fibrotic molecule to prevent endometrosis development, making noscapine a strong candidate to be applied in future endometrosis therapies.MDPIRepositório da Universidade de LisboaAmaral, AnaCebola, NélioZóstek-Mioduchowska, AnnaRebordão, Maria RosaKordowitzki, PawełSkarzynski, DariuszFerreira-Dias, Graça2023-05-21T22:20:24Z2023-02-102023-02-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/27793engAmaral A, Cebola N, Szóstek-Mioduchowska A, Rebordão MR, Kordowitzki P, Skarzynski D, Ferreira-Dias G. 2023. Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine Endometrium. International Journal Molecular Science, 24(4):3593. DOI: https://doi.org/ 10.3390/ijms2404359310.3390/ijms240435931422-0067info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-05-28T01:31:11Zoai:www.repository.utl.pt:10400.5/27793Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:56:28.646631Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine Endometrium
title Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine Endometrium
spellingShingle Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine Endometrium
Amaral, Ana
Equine
endometrosis
fibrosis
myeloperoxidase
noscapine
inhibition
collagen
title_short Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine Endometrium
title_full Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine Endometrium
title_fullStr Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine Endometrium
title_full_unstemmed Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine Endometrium
title_sort Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine Endometrium
author Amaral, Ana
author_facet Amaral, Ana
Cebola, Nélio
Zóstek-Mioduchowska, Anna
Rebordão, Maria Rosa
Kordowitzki, Paweł
Skarzynski, Dariusz
Ferreira-Dias, Graça
author_role author
author2 Cebola, Nélio
Zóstek-Mioduchowska, Anna
Rebordão, Maria Rosa
Kordowitzki, Paweł
Skarzynski, Dariusz
Ferreira-Dias, Graça
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Amaral, Ana
Cebola, Nélio
Zóstek-Mioduchowska, Anna
Rebordão, Maria Rosa
Kordowitzki, Paweł
Skarzynski, Dariusz
Ferreira-Dias, Graça
dc.subject.por.fl_str_mv Equine
endometrosis
fibrosis
myeloperoxidase
noscapine
inhibition
collagen
topic Equine
endometrosis
fibrosis
myeloperoxidase
noscapine
inhibition
collagen
description Research Areas: Biochemistry & Molecular Biology ; Chemistry
publishDate 2023
dc.date.none.fl_str_mv 2023-05-21T22:20:24Z
2023-02-10
2023-02-10T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/27793
url http://hdl.handle.net/10400.5/27793
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Amaral A, Cebola N, Szóstek-Mioduchowska A, Rebordão MR, Kordowitzki P, Skarzynski D, Ferreira-Dias G. 2023. Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine Endometrium. International Journal Molecular Science, 24(4):3593. DOI: https://doi.org/ 10.3390/ijms24043593
10.3390/ijms24043593
1422-0067
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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