Noscapine acts as a protease inhibitor of in vitro elastase-induced collagen deposition in equine endometrium

Detalhes bibliográficos
Autor(a) principal: Amaral, Ana
Data de Publicação: 2021
Outros Autores: Fernandes, Carina, Szóstek-Mioduchowska, Anna, Rebordão, Maria Rosa, Skarzynski, Dariusz Jan, Ferreira-Dias, Graça
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/23109
Resumo: Research Areas: Biochemistry & Molecular Biology ; Chemistry
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spelling Noscapine acts as a protease inhibitor of in vitro elastase-induced collagen deposition in equine endometriumEquineEndometrosisFibrosisElastaseNoscapineInhibitionCollagenResearch Areas: Biochemistry & Molecular Biology ; ChemistryABSTRACT - Endometrosis is a reproductive pathology that is responsible for mare infertility. Our recent studies have focused on the involvement of neutrophil extracellular traps enzymes, such as elastase (ELA), in the development of equine endometrosis. Noscapine (NOSC) is an alkaloid derived from poppy opium with anticough, antistroke, anticancer, and antifibrotic properties. The present work investigates the putative inhibitory in vitro effect of NOSC on collagen type I alpha 2 chain (COL1A2) mRNA and COL1 protein relative abundance induced by ELA in endometrial explants of mares in the follicular or mid-luteal phases at 24 or 48 h of treatment. The COL1A2 mRNA was evaluated by qPCR and COL1 protein relative abundance by Western blot. In equine endometrial explants, ELA increased COL 1 expression, while NOSC inhibited it at both estrous cycle phases and treatment times. These findings contribute to the future development of new endometrosis treatment approaches. Noscapine could be a drug capable of preventing collagen synthesis in mare’s endometrium and facilitate the therapeutic approach.MDPIRepositório da Universidade de LisboaAmaral, AnaFernandes, CarinaSzóstek-Mioduchowska, AnnaRebordão, Maria RosaSkarzynski, Dariusz JanFerreira-Dias, Graça2022-01-17T15:37:15Z2021-05-192021-05-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/23109engAmaral A, Fernandes C, Szóstek-Mioduchowska A, Rebordão MR, Skarzynski DJ, Ferreira-Dias G. 2021. Noscapine Acts as a Protease Inhibitor of In Vitro Elastase-Induced Collagen Deposition in Equine Endometrium. International Journal of Molecular Sciences. 22(10):5333. Doi:10.3390/ijms2210533310.3390/ijms221053331422-0067info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:52:37Zoai:www.repository.utl.pt:10400.5/23109Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:07:24.437802Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Noscapine acts as a protease inhibitor of in vitro elastase-induced collagen deposition in equine endometrium
title Noscapine acts as a protease inhibitor of in vitro elastase-induced collagen deposition in equine endometrium
spellingShingle Noscapine acts as a protease inhibitor of in vitro elastase-induced collagen deposition in equine endometrium
Amaral, Ana
Equine
Endometrosis
Fibrosis
Elastase
Noscapine
Inhibition
Collagen
title_short Noscapine acts as a protease inhibitor of in vitro elastase-induced collagen deposition in equine endometrium
title_full Noscapine acts as a protease inhibitor of in vitro elastase-induced collagen deposition in equine endometrium
title_fullStr Noscapine acts as a protease inhibitor of in vitro elastase-induced collagen deposition in equine endometrium
title_full_unstemmed Noscapine acts as a protease inhibitor of in vitro elastase-induced collagen deposition in equine endometrium
title_sort Noscapine acts as a protease inhibitor of in vitro elastase-induced collagen deposition in equine endometrium
author Amaral, Ana
author_facet Amaral, Ana
Fernandes, Carina
Szóstek-Mioduchowska, Anna
Rebordão, Maria Rosa
Skarzynski, Dariusz Jan
Ferreira-Dias, Graça
author_role author
author2 Fernandes, Carina
Szóstek-Mioduchowska, Anna
Rebordão, Maria Rosa
Skarzynski, Dariusz Jan
Ferreira-Dias, Graça
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Amaral, Ana
Fernandes, Carina
Szóstek-Mioduchowska, Anna
Rebordão, Maria Rosa
Skarzynski, Dariusz Jan
Ferreira-Dias, Graça
dc.subject.por.fl_str_mv Equine
Endometrosis
Fibrosis
Elastase
Noscapine
Inhibition
Collagen
topic Equine
Endometrosis
Fibrosis
Elastase
Noscapine
Inhibition
Collagen
description Research Areas: Biochemistry & Molecular Biology ; Chemistry
publishDate 2021
dc.date.none.fl_str_mv 2021-05-19
2021-05-19T00:00:00Z
2022-01-17T15:37:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/23109
url http://hdl.handle.net/10400.5/23109
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Amaral A, Fernandes C, Szóstek-Mioduchowska A, Rebordão MR, Skarzynski DJ, Ferreira-Dias G. 2021. Noscapine Acts as a Protease Inhibitor of In Vitro Elastase-Induced Collagen Deposition in Equine Endometrium. International Journal of Molecular Sciences. 22(10):5333. Doi:10.3390/ijms22105333
10.3390/ijms22105333
1422-0067
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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