Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/125815 |
Resumo: | LISBOA-01-0145-FEDER-402-022125 Wellcome Trust (WT099197MA GN12IF/00033/2012 GN212 |
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oai:run.unl.pt:10362/125815 |
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RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarraysCarbohydrate microarraysHelicobacter pyloriHost immune receptorsHuman seraLipopolysaccharidesMass spectrometryOrganic ChemistryPolymers and PlasticsMaterials ChemistryLISBOA-01-0145-FEDER-402-022125 Wellcome Trust (WT099197MA GN12IF/00033/2012 GN212The structural diversity of the lipopolysaccharides (LPSs) from Helicobacter pylori poses a challenge to establish accurate and strain-specific structure-function relationships in interactions with the host. Here, LPS structural domains from five clinical isolates were obtained and compared with the reference strain 26695. This was achieved combining information from structural analysis (GC-MS and ESI-MSn) with binding data after interrogation of a LPS-derived carbohydrate microarray with sequence-specific proteins. All LPSs expressed Lewisx/y and N-acetyllactosamine determinants. Ribans were also detected in LPSs from all clinical isolates, allowing their distinction from the 26695 LPS. There was evidence for 1,3-D-galactans and blood group H-type 2 sequences in two of the clinical isolates, the latter not yet described for H. pylori LPS. Furthermore, carbohydrate microarray analyses showed a strain-associated LPS recognition by the immune lectins DC-SIGN and galectin-3 and revealed distinctive LPS binding patterns by IgG antibodies in the serum from H. pylori-infected patients.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNSilva, Lisete M.Correia, Viviana G.Moreira, Ana S. P.Domingues, Maria Rosário M.Ferreira, Rui ManuelFigueiredo, CéuAzevedo, Nuno F.Marcos-Pinto, RicardoCarneiro, FátimaMagalhães, AnaReis, Celso A.Feizi, TenFerreira, José A.Coimbra, Manuel A.Palma, Angelina S.2021-10-09T04:54:21Z2021-02-012021-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/125815eng0144-8617PURE: 26743255https://doi.org/10.1016/j.carbpol.2020.117350info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:56:35Zoai:run.unl.pt:10362/125815Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:56:35Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays |
title |
Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays |
spellingShingle |
Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays Silva, Lisete M. Carbohydrate microarrays Helicobacter pylori Host immune receptors Human sera Lipopolysaccharides Mass spectrometry Organic Chemistry Polymers and Plastics Materials Chemistry |
title_short |
Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays |
title_full |
Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays |
title_fullStr |
Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays |
title_full_unstemmed |
Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays |
title_sort |
Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays |
author |
Silva, Lisete M. |
author_facet |
Silva, Lisete M. Correia, Viviana G. Moreira, Ana S. P. Domingues, Maria Rosário M. Ferreira, Rui Manuel Figueiredo, Céu Azevedo, Nuno F. Marcos-Pinto, Ricardo Carneiro, Fátima Magalhães, Ana Reis, Celso A. Feizi, Ten Ferreira, José A. Coimbra, Manuel A. Palma, Angelina S. |
author_role |
author |
author2 |
Correia, Viviana G. Moreira, Ana S. P. Domingues, Maria Rosário M. Ferreira, Rui Manuel Figueiredo, Céu Azevedo, Nuno F. Marcos-Pinto, Ricardo Carneiro, Fátima Magalhães, Ana Reis, Celso A. Feizi, Ten Ferreira, José A. Coimbra, Manuel A. Palma, Angelina S. |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
UCIBIO - Applied Molecular Biosciences Unit DQ - Departamento de Química RUN |
dc.contributor.author.fl_str_mv |
Silva, Lisete M. Correia, Viviana G. Moreira, Ana S. P. Domingues, Maria Rosário M. Ferreira, Rui Manuel Figueiredo, Céu Azevedo, Nuno F. Marcos-Pinto, Ricardo Carneiro, Fátima Magalhães, Ana Reis, Celso A. Feizi, Ten Ferreira, José A. Coimbra, Manuel A. Palma, Angelina S. |
dc.subject.por.fl_str_mv |
Carbohydrate microarrays Helicobacter pylori Host immune receptors Human sera Lipopolysaccharides Mass spectrometry Organic Chemistry Polymers and Plastics Materials Chemistry |
topic |
Carbohydrate microarrays Helicobacter pylori Host immune receptors Human sera Lipopolysaccharides Mass spectrometry Organic Chemistry Polymers and Plastics Materials Chemistry |
description |
LISBOA-01-0145-FEDER-402-022125 Wellcome Trust (WT099197MA GN12IF/00033/2012 GN212 |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-09T04:54:21Z 2021-02-01 2021-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/125815 |
url |
http://hdl.handle.net/10362/125815 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0144-8617 PURE: 26743255 https://doi.org/10.1016/j.carbpol.2020.117350 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
_version_ |
1817545824673988608 |