The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe

Detalhes bibliográficos
Autor(a) principal: Wallace, MA
Data de Publicação: 2021
Outros Autores: Coffman, KA, Gilbert, C, Ravindran, S, Albery, GF, Abbott, J, Argyridou, E, Bellosta, P, Betancourt, AJ, Colinet, H, Eric, K, Glaser-Schmitt, A, Grath, S, Jelic, M, Kankare, M, Kozeretska, I, Loeschcke, V, Montchamp-Moreau, C, Ometto, L, Onder, BS, Orengo, DJ, Parsch, J, Pascual, M, Patenkovic, A, Puerma, E, Ritchie, MG, Rota-Stabelli, O, Schou, MF, Serga, SV, Stamenkovic-Radak, M, Tanaskovic, M, Veselinovic, MS, Vieira, J, Vieira, CP, Kapun, M, Flatt, T, González, J, Staubach, F, Obbard, DJ
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/153782
Resumo: Drosophila melanogaster is an important model for antiviral immunity in arthropods, but very few DNA viruses have been described from the family Drosophilidae. This deficiency limits our opportunity to use natural host-pathogen combinations in experimental studies, and may bias our understanding of the Drosophila virome. Here, we report fourteen DNA viruses detected in a metagenomic analysis of 6668 pool-sequenced Drosophila, sampled from forty-seven European locations between 2014 and 2016. These include three new nudiviruses, a new and divergent entomopoxvirus, a virus related to Leptopilina boulardi filamentous virus, and a virus related to Musca domestica salivary gland hypertrophy virus. We also find an endogenous genomic copy of galbut virus, a double-stranded RNA partitivirus, segregating at very low frequency. Remarkably, we find that Drosophila Vesanto virus, a small DNA virus previously described as a bidnavirus, may be composed of up to twelve segments and thus represent a new lineage of segmented DNA viruses. Two of the DNA viruses, Drosophila Kallithea nudivirus and Drosophila Vesanto virus are relatively common, found in 2 per cent or more of wild flies. The others are rare, with many likely to be represented by a single infected fly. We find that virus prevalence in Europe reflects the prevalence seen in publicly available datasets, with Drosophila Kallithea nudivirus and Drosophila Vesanto virus the only ones commonly detectable in public data from wild-caught flies and large population cages, and the other viruses being rare or absent. These analyses suggest that DNA viruses are at lower prevalence than RNA viruses in D.melanogaster, and may be less likely to persist in laboratory cultures. Our findings go some way to redressing an earlier bias toward RNA virus studies in Drosophila, and lay the foundation needed to harness the power of Drosophila as a model system for the study of DNA viruses.
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spelling The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in EuropeAdintovirusBidnavirusDensovirusDNA virusDrosophilaEndogenous viral elementFilamentous virusGalbut virusNudivirusDrosophila melanogaster is an important model for antiviral immunity in arthropods, but very few DNA viruses have been described from the family Drosophilidae. This deficiency limits our opportunity to use natural host-pathogen combinations in experimental studies, and may bias our understanding of the Drosophila virome. Here, we report fourteen DNA viruses detected in a metagenomic analysis of 6668 pool-sequenced Drosophila, sampled from forty-seven European locations between 2014 and 2016. These include three new nudiviruses, a new and divergent entomopoxvirus, a virus related to Leptopilina boulardi filamentous virus, and a virus related to Musca domestica salivary gland hypertrophy virus. We also find an endogenous genomic copy of galbut virus, a double-stranded RNA partitivirus, segregating at very low frequency. Remarkably, we find that Drosophila Vesanto virus, a small DNA virus previously described as a bidnavirus, may be composed of up to twelve segments and thus represent a new lineage of segmented DNA viruses. Two of the DNA viruses, Drosophila Kallithea nudivirus and Drosophila Vesanto virus are relatively common, found in 2 per cent or more of wild flies. The others are rare, with many likely to be represented by a single infected fly. We find that virus prevalence in Europe reflects the prevalence seen in publicly available datasets, with Drosophila Kallithea nudivirus and Drosophila Vesanto virus the only ones commonly detectable in public data from wild-caught flies and large population cages, and the other viruses being rare or absent. These analyses suggest that DNA viruses are at lower prevalence than RNA viruses in D.melanogaster, and may be less likely to persist in laboratory cultures. Our findings go some way to redressing an earlier bias toward RNA virus studies in Drosophila, and lay the foundation needed to harness the power of Drosophila as a model system for the study of DNA viruses.Oxford University Press20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/153782eng2057-157710.1093/ve/veab031Wallace, MACoffman, KAGilbert, CRavindran, SAlbery, GFAbbott, JArgyridou, EBellosta, PBetancourt, AJColinet, HEric, KGlaser-Schmitt, AGrath, SJelic, MKankare, MKozeretska, ILoeschcke, VMontchamp-Moreau, COmetto, LOnder, BSOrengo, DJParsch, JPascual, MPatenkovic, APuerma, ERitchie, MGRota-Stabelli, OSchou, MFSerga, SVStamenkovic-Radak, MTanaskovic, MVeselinovic, MSVieira, JVieira, CPKapun, MFlatt, TGonzález, JStaubach, FObbard, DJinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T15:59:32Zoai:repositorio-aberto.up.pt:10216/153782Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:36:19.868399Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe
title The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe
spellingShingle The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe
Wallace, MA
Adintovirus
Bidnavirus
Densovirus
DNA virus
Drosophila
Endogenous viral element
Filamentous virus
Galbut virus
Nudivirus
title_short The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe
title_full The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe
title_fullStr The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe
title_full_unstemmed The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe
title_sort The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe
author Wallace, MA
author_facet Wallace, MA
Coffman, KA
Gilbert, C
Ravindran, S
Albery, GF
Abbott, J
Argyridou, E
Bellosta, P
Betancourt, AJ
Colinet, H
Eric, K
Glaser-Schmitt, A
Grath, S
Jelic, M
Kankare, M
Kozeretska, I
Loeschcke, V
Montchamp-Moreau, C
Ometto, L
Onder, BS
Orengo, DJ
Parsch, J
Pascual, M
Patenkovic, A
Puerma, E
Ritchie, MG
Rota-Stabelli, O
Schou, MF
Serga, SV
Stamenkovic-Radak, M
Tanaskovic, M
Veselinovic, MS
Vieira, J
Vieira, CP
Kapun, M
Flatt, T
González, J
Staubach, F
Obbard, DJ
author_role author
author2 Coffman, KA
Gilbert, C
Ravindran, S
Albery, GF
Abbott, J
Argyridou, E
Bellosta, P
Betancourt, AJ
Colinet, H
Eric, K
Glaser-Schmitt, A
Grath, S
Jelic, M
Kankare, M
Kozeretska, I
Loeschcke, V
Montchamp-Moreau, C
Ometto, L
Onder, BS
Orengo, DJ
Parsch, J
Pascual, M
Patenkovic, A
Puerma, E
Ritchie, MG
Rota-Stabelli, O
Schou, MF
Serga, SV
Stamenkovic-Radak, M
Tanaskovic, M
Veselinovic, MS
Vieira, J
Vieira, CP
Kapun, M
Flatt, T
González, J
Staubach, F
Obbard, DJ
author2_role author
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author
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author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
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author
dc.contributor.author.fl_str_mv Wallace, MA
Coffman, KA
Gilbert, C
Ravindran, S
Albery, GF
Abbott, J
Argyridou, E
Bellosta, P
Betancourt, AJ
Colinet, H
Eric, K
Glaser-Schmitt, A
Grath, S
Jelic, M
Kankare, M
Kozeretska, I
Loeschcke, V
Montchamp-Moreau, C
Ometto, L
Onder, BS
Orengo, DJ
Parsch, J
Pascual, M
Patenkovic, A
Puerma, E
Ritchie, MG
Rota-Stabelli, O
Schou, MF
Serga, SV
Stamenkovic-Radak, M
Tanaskovic, M
Veselinovic, MS
Vieira, J
Vieira, CP
Kapun, M
Flatt, T
González, J
Staubach, F
Obbard, DJ
dc.subject.por.fl_str_mv Adintovirus
Bidnavirus
Densovirus
DNA virus
Drosophila
Endogenous viral element
Filamentous virus
Galbut virus
Nudivirus
topic Adintovirus
Bidnavirus
Densovirus
DNA virus
Drosophila
Endogenous viral element
Filamentous virus
Galbut virus
Nudivirus
description Drosophila melanogaster is an important model for antiviral immunity in arthropods, but very few DNA viruses have been described from the family Drosophilidae. This deficiency limits our opportunity to use natural host-pathogen combinations in experimental studies, and may bias our understanding of the Drosophila virome. Here, we report fourteen DNA viruses detected in a metagenomic analysis of 6668 pool-sequenced Drosophila, sampled from forty-seven European locations between 2014 and 2016. These include three new nudiviruses, a new and divergent entomopoxvirus, a virus related to Leptopilina boulardi filamentous virus, and a virus related to Musca domestica salivary gland hypertrophy virus. We also find an endogenous genomic copy of galbut virus, a double-stranded RNA partitivirus, segregating at very low frequency. Remarkably, we find that Drosophila Vesanto virus, a small DNA virus previously described as a bidnavirus, may be composed of up to twelve segments and thus represent a new lineage of segmented DNA viruses. Two of the DNA viruses, Drosophila Kallithea nudivirus and Drosophila Vesanto virus are relatively common, found in 2 per cent or more of wild flies. The others are rare, with many likely to be represented by a single infected fly. We find that virus prevalence in Europe reflects the prevalence seen in publicly available datasets, with Drosophila Kallithea nudivirus and Drosophila Vesanto virus the only ones commonly detectable in public data from wild-caught flies and large population cages, and the other viruses being rare or absent. These analyses suggest that DNA viruses are at lower prevalence than RNA viruses in D.melanogaster, and may be less likely to persist in laboratory cultures. Our findings go some way to redressing an earlier bias toward RNA virus studies in Drosophila, and lay the foundation needed to harness the power of Drosophila as a model system for the study of DNA viruses.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/153782
url https://hdl.handle.net/10216/153782
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2057-1577
10.1093/ve/veab031
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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