Antimicrobial protein rBPI21-induced surface changes on Gram-negative and Gram-positive bacteria

Detalhes bibliográficos
Autor(a) principal: Domingues, Marco M.
Data de Publicação: 2014
Outros Autores: Silva, Patrícia M., Franquelim, Henri G., Carvalho, Filomena A., Castanho, Miguel A. R. B., Santos, Nuno C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/23503
Resumo: © 2014 Elsevier Inc. All rights reserved.
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spelling Antimicrobial protein rBPI21-induced surface changes on Gram-negative and Gram-positive bacteriaLipopolysacchariderBPI21Atomic force microscopy© 2014 Elsevier Inc. All rights reserved.New classes of antibiotics, such as antimicrobial peptides or proteins (AMPs), are crucial to deal with threatening bacterial diseases. rBPI21 is an AMP based on the human neutrophil BPI protein, with potential clinical use against meningitis. We studied the membrane perturbations promoted by rBPI21 on Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus. Its interaction with bacteria was also studied in the presence of lipopolysaccharide (LPS), rBPI21 major ligand. Flow cytometry analysis of both bacteria shows that rBPI21 induces membrane depolarization. rBPI21 increases the negative surface charge of both bacteria toward positive values, as shown by zeta-potential measurements. This is followed by surface perturbations, culminating in cell lysis, as visualized by atomic force microscopy (AFM). Force spectroscopy measurements show that soluble LPS decreases the interaction of rBPI21 with bacteria, especially with S. aureus. This suggests that the rBPI21 LPS-binding pocket may also participate on the binding to Gram-positive bacteria.This work was supported by Fundação para a Ciência e a Tecnologia – Ministério do Ensino e Ciência (FCT-MEC, Portugal). M.M.D. and H.G.F. acknowledge FCT-MEC PhD fellowships SFRH/BD/41750/2007 and SFRH/BD/39039/2007, respectively.ElsevierRepositório da Universidade de LisboaDomingues, Marco M.Silva, Patrícia M.Franquelim, Henri G.Carvalho, Filomena A.Castanho, Miguel A. R. B.Santos, Nuno C.2016-04-21T13:04:12Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/23503eng1549-963410.1016/j.nano.2013.11.002metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:11:32Zoai:repositorio.ul.pt:10451/23503Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:40:49.866612Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Antimicrobial protein rBPI21-induced surface changes on Gram-negative and Gram-positive bacteria
title Antimicrobial protein rBPI21-induced surface changes on Gram-negative and Gram-positive bacteria
spellingShingle Antimicrobial protein rBPI21-induced surface changes on Gram-negative and Gram-positive bacteria
Domingues, Marco M.
Lipopolysaccharide
rBPI21
Atomic force microscopy
title_short Antimicrobial protein rBPI21-induced surface changes on Gram-negative and Gram-positive bacteria
title_full Antimicrobial protein rBPI21-induced surface changes on Gram-negative and Gram-positive bacteria
title_fullStr Antimicrobial protein rBPI21-induced surface changes on Gram-negative and Gram-positive bacteria
title_full_unstemmed Antimicrobial protein rBPI21-induced surface changes on Gram-negative and Gram-positive bacteria
title_sort Antimicrobial protein rBPI21-induced surface changes on Gram-negative and Gram-positive bacteria
author Domingues, Marco M.
author_facet Domingues, Marco M.
Silva, Patrícia M.
Franquelim, Henri G.
Carvalho, Filomena A.
Castanho, Miguel A. R. B.
Santos, Nuno C.
author_role author
author2 Silva, Patrícia M.
Franquelim, Henri G.
Carvalho, Filomena A.
Castanho, Miguel A. R. B.
Santos, Nuno C.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Domingues, Marco M.
Silva, Patrícia M.
Franquelim, Henri G.
Carvalho, Filomena A.
Castanho, Miguel A. R. B.
Santos, Nuno C.
dc.subject.por.fl_str_mv Lipopolysaccharide
rBPI21
Atomic force microscopy
topic Lipopolysaccharide
rBPI21
Atomic force microscopy
description © 2014 Elsevier Inc. All rights reserved.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
2016-04-21T13:04:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/23503
url http://hdl.handle.net/10451/23503
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1549-9634
10.1016/j.nano.2013.11.002
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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