Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation

Detalhes bibliográficos
Autor(a) principal: Ribeiro Da Cunha, Bernardo
Data de Publicação: 2020
Outros Autores: Fonseca, Luís P. P., Calado, Cecília
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/11938
Resumo: Este trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2017 do Instituto Politécnico de Lisboa. Código de referência IPL/2017/DrugsPlatf/ISEL
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spelling Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidationAntibiotic discoveryChemometricsEscherichia coliFourier-Transform infrared (FTIR) spectroscopyHigh-throughput screeningMechanism-of-action (MOA)Metabolic fingerprintingMultivariate analysisEste trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2017 do Instituto Politécnico de Lisboa. Código de referência IPL/2017/DrugsPlatf/ISELEste trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2018 do Instituto Politécnico de Lisboa. Código de referência IPL/2018/RENALPROG_ISELThe discovery of antibiotics has been slowing to a halt. Phenotypic screening is once again at the forefront of antibiotic discovery, yet Mechanism-Of-Action (MOA) identification is still a major bottleneck. As such, methods capable of MOA elucidation coupled with the high-throughput screening of whole cells are required now more than ever, for which Fourier-Transform Infrared (FTIR) spectroscopy is a promising metabolic fingerprinting technique. A high-throughput whole-cell FTIR spectroscopy-based bioassay was developed to reveal the metabolic fingerprint induced by 15 antibiotics on the Escherichia coli metabolism. Cells were briefly exposed to four times the minimum inhibitory concentration and spectra were quickly acquired in the high-throughput mode. After preprocessing optimization, a partial least squares discriminant analysis and principal component analysis were conducted. The metabolic fingerprints obtained with FTIR spectroscopy were sufficiently specific to allow a clear distinction between different antibiotics, across three independent cultures, with either analysis algorithm. These fingerprints were coherent with the known MOA of all the antibiotics tested, which include examples that target the protein, DNA, RNA, and cell wall biosynthesis. Because FTIR spectroscopy acquires a holistic fingerprint of the effect of antibiotics on the cellular metabolism, it holds great potential to be used for high-throughput screening in antibiotic discovery and possibly towards a better understanding of the MOA of current antibiotics.MDPIRCIPLRibeiro Da Cunha, BernardoFonseca, Luís P. P.Calado, Cecília2020-06-22T14:14:02Z2020-04-092020-04-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/11938engCUNHA, Bernardo Ribeiro da; FONSECA, Luís P.; CALADO, Cecília R. C. – Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation. Metabolites. ISSN 2218-1989. Vol. 10, N.º 4 (2020), pp. 1-152218-198910.3390/metabo10040145info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:03:54Zoai:repositorio.ipl.pt:10400.21/11938Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:20:07.432805Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation
title Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation
spellingShingle Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation
Ribeiro Da Cunha, Bernardo
Antibiotic discovery
Chemometrics
Escherichia coli
Fourier-Transform infrared (FTIR) spectroscopy
High-throughput screening
Mechanism-of-action (MOA)
Metabolic fingerprinting
Multivariate analysis
title_short Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation
title_full Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation
title_fullStr Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation
title_full_unstemmed Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation
title_sort Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation
author Ribeiro Da Cunha, Bernardo
author_facet Ribeiro Da Cunha, Bernardo
Fonseca, Luís P. P.
Calado, Cecília
author_role author
author2 Fonseca, Luís P. P.
Calado, Cecília
author2_role author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Ribeiro Da Cunha, Bernardo
Fonseca, Luís P. P.
Calado, Cecília
dc.subject.por.fl_str_mv Antibiotic discovery
Chemometrics
Escherichia coli
Fourier-Transform infrared (FTIR) spectroscopy
High-throughput screening
Mechanism-of-action (MOA)
Metabolic fingerprinting
Multivariate analysis
topic Antibiotic discovery
Chemometrics
Escherichia coli
Fourier-Transform infrared (FTIR) spectroscopy
High-throughput screening
Mechanism-of-action (MOA)
Metabolic fingerprinting
Multivariate analysis
description Este trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2017 do Instituto Politécnico de Lisboa. Código de referência IPL/2017/DrugsPlatf/ISEL
publishDate 2020
dc.date.none.fl_str_mv 2020-06-22T14:14:02Z
2020-04-09
2020-04-09T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/11938
url http://hdl.handle.net/10400.21/11938
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv CUNHA, Bernardo Ribeiro da; FONSECA, Luís P.; CALADO, Cecília R. C. – Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation. Metabolites. ISSN 2218-1989. Vol. 10, N.º 4 (2020), pp. 1-15
2218-1989
10.3390/metabo10040145
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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