Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.21/11938 |
Resumo: | Este trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2017 do Instituto Politécnico de Lisboa. Código de referência IPL/2017/DrugsPlatf/ISEL |
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Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidationAntibiotic discoveryChemometricsEscherichia coliFourier-Transform infrared (FTIR) spectroscopyHigh-throughput screeningMechanism-of-action (MOA)Metabolic fingerprintingMultivariate analysisEste trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2017 do Instituto Politécnico de Lisboa. Código de referência IPL/2017/DrugsPlatf/ISELEste trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2018 do Instituto Politécnico de Lisboa. Código de referência IPL/2018/RENALPROG_ISELThe discovery of antibiotics has been slowing to a halt. Phenotypic screening is once again at the forefront of antibiotic discovery, yet Mechanism-Of-Action (MOA) identification is still a major bottleneck. As such, methods capable of MOA elucidation coupled with the high-throughput screening of whole cells are required now more than ever, for which Fourier-Transform Infrared (FTIR) spectroscopy is a promising metabolic fingerprinting technique. A high-throughput whole-cell FTIR spectroscopy-based bioassay was developed to reveal the metabolic fingerprint induced by 15 antibiotics on the Escherichia coli metabolism. Cells were briefly exposed to four times the minimum inhibitory concentration and spectra were quickly acquired in the high-throughput mode. After preprocessing optimization, a partial least squares discriminant analysis and principal component analysis were conducted. The metabolic fingerprints obtained with FTIR spectroscopy were sufficiently specific to allow a clear distinction between different antibiotics, across three independent cultures, with either analysis algorithm. These fingerprints were coherent with the known MOA of all the antibiotics tested, which include examples that target the protein, DNA, RNA, and cell wall biosynthesis. Because FTIR spectroscopy acquires a holistic fingerprint of the effect of antibiotics on the cellular metabolism, it holds great potential to be used for high-throughput screening in antibiotic discovery and possibly towards a better understanding of the MOA of current antibiotics.MDPIRCIPLRibeiro Da Cunha, BernardoFonseca, Luís P. P.Calado, Cecília2020-06-22T14:14:02Z2020-04-092020-04-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/11938engCUNHA, Bernardo Ribeiro da; FONSECA, Luís P.; CALADO, Cecília R. C. – Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation. Metabolites. ISSN 2218-1989. Vol. 10, N.º 4 (2020), pp. 1-152218-198910.3390/metabo10040145info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:03:54Zoai:repositorio.ipl.pt:10400.21/11938Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:20:07.432805Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation |
title |
Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation |
spellingShingle |
Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation Ribeiro Da Cunha, Bernardo Antibiotic discovery Chemometrics Escherichia coli Fourier-Transform infrared (FTIR) spectroscopy High-throughput screening Mechanism-of-action (MOA) Metabolic fingerprinting Multivariate analysis |
title_short |
Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation |
title_full |
Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation |
title_fullStr |
Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation |
title_full_unstemmed |
Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation |
title_sort |
Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation |
author |
Ribeiro Da Cunha, Bernardo |
author_facet |
Ribeiro Da Cunha, Bernardo Fonseca, Luís P. P. Calado, Cecília |
author_role |
author |
author2 |
Fonseca, Luís P. P. Calado, Cecília |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
RCIPL |
dc.contributor.author.fl_str_mv |
Ribeiro Da Cunha, Bernardo Fonseca, Luís P. P. Calado, Cecília |
dc.subject.por.fl_str_mv |
Antibiotic discovery Chemometrics Escherichia coli Fourier-Transform infrared (FTIR) spectroscopy High-throughput screening Mechanism-of-action (MOA) Metabolic fingerprinting Multivariate analysis |
topic |
Antibiotic discovery Chemometrics Escherichia coli Fourier-Transform infrared (FTIR) spectroscopy High-throughput screening Mechanism-of-action (MOA) Metabolic fingerprinting Multivariate analysis |
description |
Este trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2017 do Instituto Politécnico de Lisboa. Código de referência IPL/2017/DrugsPlatf/ISEL |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06-22T14:14:02Z 2020-04-09 2020-04-09T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.21/11938 |
url |
http://hdl.handle.net/10400.21/11938 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
CUNHA, Bernardo Ribeiro da; FONSECA, Luís P.; CALADO, Cecília R. C. – Metabolic fingerprinting with Fourier-Transform Infrared (FTIR) spectroscopy: towards a high-throughput screening assay for antibiotic discovery and mechanism-of-action elucidation. Metabolites. ISSN 2218-1989. Vol. 10, N.º 4 (2020), pp. 1-15 2218-1989 10.3390/metabo10040145 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133469342695424 |