The genetic structure of fermentative vineyard-associated Saccharomyces cerevisiae populations revealed by microsatellite analysis

Detalhes bibliográficos
Autor(a) principal: Schuller, Dorit Elisabeth
Data de Publicação: 2007
Outros Autores: Casal, Margarida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/6486
Resumo: Prova tipográfica (In Press)
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spelling The genetic structure of fermentative vineyard-associated Saccharomyces cerevisiae populations revealed by microsatellite analysisGenetic structureSaccharomyces cerevisiaeMicrosatelliteYeast populationBiodiversityVineyardActive dry yeastProva tipográfica (In Press)From the analysis of six polymorphic microsatellite loci performed in 361 Saccharomyces cerevisiae isolates 93 alleles were identified, being 52 of them described for the first time. All these isolates have a distinct mtDNA RFLP pattern. They are derived from a pool of 1620 isolates obtained from spontaneous fermentations of grapes collected in three vineyards of the Vinho Verde Region in Portugal, during the 2001 – 2003 harvest seasons. For all loci analyzed, observed heterozygosity was three to four times lower than the expected value supposing a Hardy-Weinberg equilibrium (random mating and no evolutionary mechanisms acting), indicating a clonal structure and strong populational substructuring. Genetic differences among S. cerevisiae populations were apparent mainly from gradations in allele frequencies rather than from distinctive “diagnostic” genotypes, and the accumulation of small allele-frequency differences across six loci allowed the identification of population structures. Genetic differentiation in the same vineyard in consecutive years was of the same order of magnitude as the differences verified among the diferent vineyards. Correlation of genetic differentiation with the distance between sampling points within a vineyard suggested a pattern of isolation-by-distance, where genetic divergence in a vineyard increased with size. The continuous use of commercial yeasts has a limited influence on the autochthonous fermentative yeast population collected from grapes and may just slightly change populational structures of strains isolated from sites very close to the winery where they have been used. The present work is the first large-scale approach using microsatellite typing allowing a very fine resolution of indigenous S. cerevisiae populations isolated from vineyards.This study was supported by the project ENOSAFE (Nº 762, Programa AGRO, medida 8), the programme POCI 2010 (project POCI/AGR/56771/2004) and the grant nº 657 C2 from the cooperation agreement between the Portuguese Institute for International Scientific and Technological Cooperation (ICCTI) and the French Embassy in Lisbon. We wish to thank Prof. Célia Pais and for helpful discussions during the preparation of this manuscript. Prof. Paula Sampaio is kindly thanked for assistance with statistical analysis. Magda Silva Graça is gratefully acknowledged for the operation of the DNA sequencer. We also appreciate the kind assistance of the enologists Rui Cunha, Anselmo Mendes, Euclides Rodrigues and José Domingues for facilitating sampling campaigns in the three vineyards.SpringerUniversidade do MinhoSchuller, Dorit ElisabethCasal, Margarida20072007-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/6486eng0003-6072info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:26:01Zoai:repositorium.sdum.uminho.pt:1822/6486Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:20:21.240619Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The genetic structure of fermentative vineyard-associated Saccharomyces cerevisiae populations revealed by microsatellite analysis
title The genetic structure of fermentative vineyard-associated Saccharomyces cerevisiae populations revealed by microsatellite analysis
spellingShingle The genetic structure of fermentative vineyard-associated Saccharomyces cerevisiae populations revealed by microsatellite analysis
Schuller, Dorit Elisabeth
Genetic structure
Saccharomyces cerevisiae
Microsatellite
Yeast population
Biodiversity
Vineyard
Active dry yeast
title_short The genetic structure of fermentative vineyard-associated Saccharomyces cerevisiae populations revealed by microsatellite analysis
title_full The genetic structure of fermentative vineyard-associated Saccharomyces cerevisiae populations revealed by microsatellite analysis
title_fullStr The genetic structure of fermentative vineyard-associated Saccharomyces cerevisiae populations revealed by microsatellite analysis
title_full_unstemmed The genetic structure of fermentative vineyard-associated Saccharomyces cerevisiae populations revealed by microsatellite analysis
title_sort The genetic structure of fermentative vineyard-associated Saccharomyces cerevisiae populations revealed by microsatellite analysis
author Schuller, Dorit Elisabeth
author_facet Schuller, Dorit Elisabeth
Casal, Margarida
author_role author
author2 Casal, Margarida
author2_role author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Schuller, Dorit Elisabeth
Casal, Margarida
dc.subject.por.fl_str_mv Genetic structure
Saccharomyces cerevisiae
Microsatellite
Yeast population
Biodiversity
Vineyard
Active dry yeast
topic Genetic structure
Saccharomyces cerevisiae
Microsatellite
Yeast population
Biodiversity
Vineyard
Active dry yeast
description Prova tipográfica (In Press)
publishDate 2007
dc.date.none.fl_str_mv 2007
2007-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/6486
url https://hdl.handle.net/1822/6486
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0003-6072
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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