Genetic characterization of commercial saccharomyces cerevisiae isolates recovered from vineyard environments

Detalhes bibliográficos
Autor(a) principal: Schuller, Dorit Elisabeth
Data de Publicação: 2007
Outros Autores: Pereira, Leonor, Alves, Hugo, Cambon, Brigitte, Dequin, Sylvie, Casal, Margarida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/7555
Resumo: One hundred isolates of the commercial S. cerevisiae strain Zymaflore VL1 were recovered from spontaneous fermentations carried out with grapes collected from vineyards located close to wineries in the Vinho Verde Wine Region (Portugal). Isolates were differentiated based on their mitochondrial DNA restriction patterns and the evaluation of genetic polymorphisms was carried out by microsatellite analysis, interdelta sequence typing and pulse field gel electrophoresis. Genetic patterns were compared to the ones obtained for 30 isolates of the original commercialized Zymaflore VL1 strain. Among the 100 recovered isolates we found a high percentage of chromosomal size variations, most evident for the smaller chromosomes III and VI. Complete loss of heterozygosity was observed for two isolates that also lost chromosomal heteromorphism; their growth and fermentative capacity in a synthetic must medium was also affected. Considerably higher number of variant patterns for interdelta sequence amplifications was obtained for grape-derived strains compared to the original VL1 isolates. Our data show that the permanence of strain VL1 in natural grapevine environments induced genetic changes that can be detected different fingerprinting methods. The observed genetic changes may reflect adaptive mechanisms to changed environmental conditions that yeast cells encounter during their permanence in nature.
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spelling Genetic characterization of commercial saccharomyces cerevisiae isolates recovered from vineyard environmentsSaccharomyces cerevisiaeWine yeastMicrosatelliteMitochondrial DNA RFLPElectrophoretic karyotypingInterdelta sequenceVinho verde wine regionScience & TechnologyOne hundred isolates of the commercial S. cerevisiae strain Zymaflore VL1 were recovered from spontaneous fermentations carried out with grapes collected from vineyards located close to wineries in the Vinho Verde Wine Region (Portugal). Isolates were differentiated based on their mitochondrial DNA restriction patterns and the evaluation of genetic polymorphisms was carried out by microsatellite analysis, interdelta sequence typing and pulse field gel electrophoresis. Genetic patterns were compared to the ones obtained for 30 isolates of the original commercialized Zymaflore VL1 strain. Among the 100 recovered isolates we found a high percentage of chromosomal size variations, most evident for the smaller chromosomes III and VI. Complete loss of heterozygosity was observed for two isolates that also lost chromosomal heteromorphism; their growth and fermentative capacity in a synthetic must medium was also affected. Considerably higher number of variant patterns for interdelta sequence amplifications was obtained for grape-derived strains compared to the original VL1 isolates. Our data show that the permanence of strain VL1 in natural grapevine environments induced genetic changes that can be detected different fingerprinting methods. The observed genetic changes may reflect adaptive mechanisms to changed environmental conditions that yeast cells encounter during their permanence in nature.This study was funded by the programs POCI 2010 (FEDER/FCT, POCI/AGR/56102/2004) and AGRO (ENOSAFE, No. 762), Portugal.John Wiley & Sons LtdUniversidade do MinhoSchuller, Dorit ElisabethPereira, LeonorAlves, HugoCambon, BrigitteDequin, SylvieCasal, Margarida2007-082007-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/7555eng"Yeast". ISSN 1097-0061. 24:8 (Aug. 2007) 625-636.1097-006110.1002/yea.149617534867info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:21:11Zoai:repositorium.sdum.uminho.pt:1822/7555Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:14:23.710179Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genetic characterization of commercial saccharomyces cerevisiae isolates recovered from vineyard environments
title Genetic characterization of commercial saccharomyces cerevisiae isolates recovered from vineyard environments
spellingShingle Genetic characterization of commercial saccharomyces cerevisiae isolates recovered from vineyard environments
Schuller, Dorit Elisabeth
Saccharomyces cerevisiae
Wine yeast
Microsatellite
Mitochondrial DNA RFLP
Electrophoretic karyotyping
Interdelta sequence
Vinho verde wine region
Science & Technology
title_short Genetic characterization of commercial saccharomyces cerevisiae isolates recovered from vineyard environments
title_full Genetic characterization of commercial saccharomyces cerevisiae isolates recovered from vineyard environments
title_fullStr Genetic characterization of commercial saccharomyces cerevisiae isolates recovered from vineyard environments
title_full_unstemmed Genetic characterization of commercial saccharomyces cerevisiae isolates recovered from vineyard environments
title_sort Genetic characterization of commercial saccharomyces cerevisiae isolates recovered from vineyard environments
author Schuller, Dorit Elisabeth
author_facet Schuller, Dorit Elisabeth
Pereira, Leonor
Alves, Hugo
Cambon, Brigitte
Dequin, Sylvie
Casal, Margarida
author_role author
author2 Pereira, Leonor
Alves, Hugo
Cambon, Brigitte
Dequin, Sylvie
Casal, Margarida
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Schuller, Dorit Elisabeth
Pereira, Leonor
Alves, Hugo
Cambon, Brigitte
Dequin, Sylvie
Casal, Margarida
dc.subject.por.fl_str_mv Saccharomyces cerevisiae
Wine yeast
Microsatellite
Mitochondrial DNA RFLP
Electrophoretic karyotyping
Interdelta sequence
Vinho verde wine region
Science & Technology
topic Saccharomyces cerevisiae
Wine yeast
Microsatellite
Mitochondrial DNA RFLP
Electrophoretic karyotyping
Interdelta sequence
Vinho verde wine region
Science & Technology
description One hundred isolates of the commercial S. cerevisiae strain Zymaflore VL1 were recovered from spontaneous fermentations carried out with grapes collected from vineyards located close to wineries in the Vinho Verde Wine Region (Portugal). Isolates were differentiated based on their mitochondrial DNA restriction patterns and the evaluation of genetic polymorphisms was carried out by microsatellite analysis, interdelta sequence typing and pulse field gel electrophoresis. Genetic patterns were compared to the ones obtained for 30 isolates of the original commercialized Zymaflore VL1 strain. Among the 100 recovered isolates we found a high percentage of chromosomal size variations, most evident for the smaller chromosomes III and VI. Complete loss of heterozygosity was observed for two isolates that also lost chromosomal heteromorphism; their growth and fermentative capacity in a synthetic must medium was also affected. Considerably higher number of variant patterns for interdelta sequence amplifications was obtained for grape-derived strains compared to the original VL1 isolates. Our data show that the permanence of strain VL1 in natural grapevine environments induced genetic changes that can be detected different fingerprinting methods. The observed genetic changes may reflect adaptive mechanisms to changed environmental conditions that yeast cells encounter during their permanence in nature.
publishDate 2007
dc.date.none.fl_str_mv 2007-08
2007-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/7555
url https://hdl.handle.net/1822/7555
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Yeast". ISSN 1097-0061. 24:8 (Aug. 2007) 625-636.
1097-0061
10.1002/yea.1496
17534867
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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