Unraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophages

Detalhes bibliográficos
Autor(a) principal: Ferreira, Sílvia A.
Data de Publicação: 2012
Outros Autores: Correia, Alexandra, Madureira, Pedro, Vilanova, Manuel, Gama, F. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/22306
Resumo: The mechanisms associated with the cellular internalization of nanomedicines must be carefully considered when designing drug- and vaccine-delivery systems. The cellular fate and effects of nanomedicines depend to a large extent on the cell uptake routes. A self-assembled mannan nanogel is developed as a vaccination platform for antigen and adjuvant delivery. The mannan nanogel uptake by murine bone-marrow-derived macrophages is found to be time-, concentration-, and energy-dependent, involving mannose-receptor-mediated phagocytosis and clathrin-mediated endocytosis. The nanogel is also visualized in the cytosol suggesting endolysosomal escape. These results indicate that mannan nanogel is a promising versatile carrier for intracellular delivery of vaccines or therapeutic agents.
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spelling Unraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophagesNanogelCell internalizationConfocal microscopyNanogelCellendocytosisimagingmannan nanogelself-assemblyScience & TechnologyThe mechanisms associated with the cellular internalization of nanomedicines must be carefully considered when designing drug- and vaccine-delivery systems. The cellular fate and effects of nanomedicines depend to a large extent on the cell uptake routes. A self-assembled mannan nanogel is developed as a vaccination platform for antigen and adjuvant delivery. The mannan nanogel uptake by murine bone-marrow-derived macrophages is found to be time-, concentration-, and energy-dependent, involving mannose-receptor-mediated phagocytosis and clathrin-mediated endocytosis. The nanogel is also visualized in the cytosol suggesting endolysosomal escape. These results indicate that mannan nanogel is a promising versatile carrier for intracellular delivery of vaccines or therapeutic agents.S. A. F. is the recipient of a fellowship from International Iberian Nanotechnology Laboratory (INL). The authors wish to acknowledge the financial support from the Fundacao para a Ciencia e a Tecnologia (FCT, Portugal), trough PTDC. M. V., P.o M. and A. C. were supported by SUDOE-FEDER (IMMUNONET, SOE1/P1/E014). The technical support on confocal studies by Lucilia Goreti Ribeiro Pinto from Life and Health Sciences Research Institute (ICVS), School of Health Sciences, Braga, Portugal, is gratefully acknowledged. The authors thankfully acknowledge the important scientific comments, helpful advice and critical review given by Prof. Dr. Arwyn Tomos Jones, Welsh School of Pharmacy, Cardiff University, Cardiff.John Wiley and SonsUniversidade do MinhoFerreira, Sílvia A.Correia, AlexandraMadureira, PedroVilanova, ManuelGama, F. M.20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/22306eng1616-518710.1002/mabi.20120007522807263info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:52:41Zoai:repositorium.sdum.uminho.pt:1822/22306Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:51:52.225468Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Unraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophages
title Unraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophages
spellingShingle Unraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophages
Ferreira, Sílvia A.
Nanogel
Cell internalization
Confocal microscopy
NanogelCell
endocytosis
imaging
mannan nanogel
self-assembly
Science & Technology
title_short Unraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophages
title_full Unraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophages
title_fullStr Unraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophages
title_full_unstemmed Unraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophages
title_sort Unraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophages
author Ferreira, Sílvia A.
author_facet Ferreira, Sílvia A.
Correia, Alexandra
Madureira, Pedro
Vilanova, Manuel
Gama, F. M.
author_role author
author2 Correia, Alexandra
Madureira, Pedro
Vilanova, Manuel
Gama, F. M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Ferreira, Sílvia A.
Correia, Alexandra
Madureira, Pedro
Vilanova, Manuel
Gama, F. M.
dc.subject.por.fl_str_mv Nanogel
Cell internalization
Confocal microscopy
NanogelCell
endocytosis
imaging
mannan nanogel
self-assembly
Science & Technology
topic Nanogel
Cell internalization
Confocal microscopy
NanogelCell
endocytosis
imaging
mannan nanogel
self-assembly
Science & Technology
description The mechanisms associated with the cellular internalization of nanomedicines must be carefully considered when designing drug- and vaccine-delivery systems. The cellular fate and effects of nanomedicines depend to a large extent on the cell uptake routes. A self-assembled mannan nanogel is developed as a vaccination platform for antigen and adjuvant delivery. The mannan nanogel uptake by murine bone-marrow-derived macrophages is found to be time-, concentration-, and energy-dependent, involving mannose-receptor-mediated phagocytosis and clathrin-mediated endocytosis. The nanogel is also visualized in the cytosol suggesting endolysosomal escape. These results indicate that mannan nanogel is a promising versatile carrier for intracellular delivery of vaccines or therapeutic agents.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/22306
url http://hdl.handle.net/1822/22306
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1616-5187
10.1002/mabi.201200075
22807263
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley and Sons
publisher.none.fl_str_mv John Wiley and Sons
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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