Biocompatibility of a self-assembled glycol chitosan nanogel
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/34595 |
Resumo: | The research of chitosan-based nanogel for biomedical applications has grown exponentially in the last years; however, its biocompatibility is still insufficiently reported. Hence, the present work provides a thorough study of the biocompatibility of a glycol chitosan (GC) nanogel. The obtained results showed that GC nanogel induced slight decrease on metabolic activity of RAW, 3T3 and HMEC cell cultures, although no effect on cell membrane integrity was verified. The nanogel does not promote cell death by apoptosis and/or necrosis, exception made for the HMEC cell line challenged with the higher GC nanogel concentration. Cell cycle arrest on G1 phase was observed only in the case of RAW cells. Remarkably, the nanogel is poorly internalized by bone marrow derived macrophages and does not trigger the activation of the complement system. GC nanogel blood compatibility was confirmed through haemolysis and whole blood clotting time assays. Overall, the results demonstrated the safety of the use of the GC nanogel as drug delivery system. |
id |
RCAP_5e1859818964740328aeda2f1cb36d8b |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/34595 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Biocompatibility of a self-assembled glycol chitosan nanogelGlycol chitosan nanogelCytotoxicityApoptosisCell cycleComplement activationHaemocompatibilityToxicologyNanogelGlycol chitosanScience & TechnologyThe research of chitosan-based nanogel for biomedical applications has grown exponentially in the last years; however, its biocompatibility is still insufficiently reported. Hence, the present work provides a thorough study of the biocompatibility of a glycol chitosan (GC) nanogel. The obtained results showed that GC nanogel induced slight decrease on metabolic activity of RAW, 3T3 and HMEC cell cultures, although no effect on cell membrane integrity was verified. The nanogel does not promote cell death by apoptosis and/or necrosis, exception made for the HMEC cell line challenged with the higher GC nanogel concentration. Cell cycle arrest on G1 phase was observed only in the case of RAW cells. Remarkably, the nanogel is poorly internalized by bone marrow derived macrophages and does not trigger the activation of the complement system. GC nanogel blood compatibility was confirmed through haemolysis and whole blood clotting time assays. Overall, the results demonstrated the safety of the use of the GC nanogel as drug delivery system.Paula Pereira thanks FCT, the Ph.D. grant ref SFRH/BD/64977/2009. This work was also supported by a grant from the Spanish Ministry of Economy and Competitivity (SAF2011-30337-C02-02). We also acknowledge the European Union Seventh Framework Programme [FP7/REGPOT-2012-2013.1] under grant agreement BIOCAPS-316265. MP acknowledges fellowship from Spanish Ministry of Education (FPU predoctoral grant program).ElsevierUniversidade do MinhoPereira, PaulaPedrosa, Sílvia SantosCorreia, AlexandraLima, Cristóvão F.Peleteiro Olmedo, MercedesGonzález-Fernández, ÁfricaVilanova, ManuelGama, F. M.20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/34595engPereira, Paula; Pedrosa, S. S.; Correia, Alexandra; Lima, Cristovão F.; Olmedo, Mercedes P.; González-Fernández, África; Vilanova, M.; Gama, F. M., Biocompatibility of a self-assembled glycol chitosan nanogel. Toxicology in Vitro, 29(3), 638-646, 20150887-233310.1016/j.tiv.2014.11.00425482991info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:00:27Zoai:repositorium.sdum.uminho.pt:1822/34595Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:50:20.951730Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Biocompatibility of a self-assembled glycol chitosan nanogel |
title |
Biocompatibility of a self-assembled glycol chitosan nanogel |
spellingShingle |
Biocompatibility of a self-assembled glycol chitosan nanogel Pereira, Paula Glycol chitosan nanogel Cytotoxicity Apoptosis Cell cycle Complement activation Haemocompatibility Toxicology Nanogel Glycol chitosan Science & Technology |
title_short |
Biocompatibility of a self-assembled glycol chitosan nanogel |
title_full |
Biocompatibility of a self-assembled glycol chitosan nanogel |
title_fullStr |
Biocompatibility of a self-assembled glycol chitosan nanogel |
title_full_unstemmed |
Biocompatibility of a self-assembled glycol chitosan nanogel |
title_sort |
Biocompatibility of a self-assembled glycol chitosan nanogel |
author |
Pereira, Paula |
author_facet |
Pereira, Paula Pedrosa, Sílvia Santos Correia, Alexandra Lima, Cristóvão F. Peleteiro Olmedo, Mercedes González-Fernández, África Vilanova, Manuel Gama, F. M. |
author_role |
author |
author2 |
Pedrosa, Sílvia Santos Correia, Alexandra Lima, Cristóvão F. Peleteiro Olmedo, Mercedes González-Fernández, África Vilanova, Manuel Gama, F. M. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pereira, Paula Pedrosa, Sílvia Santos Correia, Alexandra Lima, Cristóvão F. Peleteiro Olmedo, Mercedes González-Fernández, África Vilanova, Manuel Gama, F. M. |
dc.subject.por.fl_str_mv |
Glycol chitosan nanogel Cytotoxicity Apoptosis Cell cycle Complement activation Haemocompatibility Toxicology Nanogel Glycol chitosan Science & Technology |
topic |
Glycol chitosan nanogel Cytotoxicity Apoptosis Cell cycle Complement activation Haemocompatibility Toxicology Nanogel Glycol chitosan Science & Technology |
description |
The research of chitosan-based nanogel for biomedical applications has grown exponentially in the last years; however, its biocompatibility is still insufficiently reported. Hence, the present work provides a thorough study of the biocompatibility of a glycol chitosan (GC) nanogel. The obtained results showed that GC nanogel induced slight decrease on metabolic activity of RAW, 3T3 and HMEC cell cultures, although no effect on cell membrane integrity was verified. The nanogel does not promote cell death by apoptosis and/or necrosis, exception made for the HMEC cell line challenged with the higher GC nanogel concentration. Cell cycle arrest on G1 phase was observed only in the case of RAW cells. Remarkably, the nanogel is poorly internalized by bone marrow derived macrophages and does not trigger the activation of the complement system. GC nanogel blood compatibility was confirmed through haemolysis and whole blood clotting time assays. Overall, the results demonstrated the safety of the use of the GC nanogel as drug delivery system. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/34595 |
url |
http://hdl.handle.net/1822/34595 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pereira, Paula; Pedrosa, S. S.; Correia, Alexandra; Lima, Cristovão F.; Olmedo, Mercedes P.; González-Fernández, África; Vilanova, M.; Gama, F. M., Biocompatibility of a self-assembled glycol chitosan nanogel. Toxicology in Vitro, 29(3), 638-646, 2015 0887-2333 10.1016/j.tiv.2014.11.004 25482991 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132271717908480 |