Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water

Detalhes bibliográficos
Autor(a) principal: Ferreira, A.C.
Data de Publicação: 1996
Outros Autores: Morais, Paula V, Alcobia Gomes, Célia Margarida, Da Costa, Milton
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.26/33490
Resumo: The microflora of a natural mineral water was studied immediately after bottling (T0) and after 7 d storage (T7) during 6 months, and isolates were clustered by SDS-PAGE of whole-cell protein profiles. Isolates from each cluster were further characterized by API 20NE, fatty acid composition and quinone profiles. The numerical analysis of the electrophoregrams of all bacteria isolated from the mineral water formed 15 clusters and five unclustered strains. Except for five minor clusters, all clusters were composed of strains isolated over several months. The numerical analysis of the electrophoregrams of bacteria isolated immediately after bottling formed 15 clusters while after 7 d storage only four of these populations could be isolated, indicating that populations present in the mineral water were stable and that changes occurring after bottling probably resulted from a selection process. Only one unclustered strain was identified simultaneously by all the systems, as Sphingomonas paucimobilis. The monitoring of the aquifer and the bottling system, and the construction of a large database with bacteria of the autochthonous flora allows the detection of alterations in the aquifer by changes in the microflora.
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spelling Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral waterBacteriaBacterial proteinsFatty acidsMineral watersReproducibility of resultsTime factorsElectrophoresis, polyacrylamide gelNumerical analysis, computer-assistedQuinonesBactériasProteínas de bactériasÁcidos gordosÁguas mineraisReprodutibilidade dos resultadosEletroforese em gel de poliacrilamidaAnálise numérica assistida por computadorQuinonasFatores de tempoThe microflora of a natural mineral water was studied immediately after bottling (T0) and after 7 d storage (T7) during 6 months, and isolates were clustered by SDS-PAGE of whole-cell protein profiles. Isolates from each cluster were further characterized by API 20NE, fatty acid composition and quinone profiles. The numerical analysis of the electrophoregrams of all bacteria isolated from the mineral water formed 15 clusters and five unclustered strains. Except for five minor clusters, all clusters were composed of strains isolated over several months. The numerical analysis of the electrophoregrams of bacteria isolated immediately after bottling formed 15 clusters while after 7 d storage only four of these populations could be isolated, indicating that populations present in the mineral water were stable and that changes occurring after bottling probably resulted from a selection process. Only one unclustered strain was identified simultaneously by all the systems, as Sphingomonas paucimobilis. The monitoring of the aquifer and the bottling system, and the construction of a large database with bacteria of the autochthonous flora allows the detection of alterations in the aquifer by changes in the microflora.Wiley BlackwellRepositório ComumFerreira, A.C.Morais, Paula VAlcobia Gomes, Célia MargaridaDa Costa, Milton2020-10-07T12:05:31Z1996-05-01T00:00:00Z1996-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.26/33490eng0021-884710.1111/j.1365-2672.1996.tb03246.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-05T15:41:12Zoai:comum.rcaap.pt:10400.26/33490Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:16:58.894746Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water
title Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water
spellingShingle Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water
Ferreira, A.C.
Bacteria
Bacterial proteins
Fatty acids
Mineral waters
Reproducibility of results
Time factors
Electrophoresis, polyacrylamide gel
Numerical analysis, computer-assisted
Quinones
Bactérias
Proteínas de bactérias
Ácidos gordos
Águas minerais
Reprodutibilidade dos resultados
Eletroforese em gel de poliacrilamida
Análise numérica assistida por computador
Quinonas
Fatores de tempo
title_short Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water
title_full Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water
title_fullStr Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water
title_full_unstemmed Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water
title_sort Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water
author Ferreira, A.C.
author_facet Ferreira, A.C.
Morais, Paula V
Alcobia Gomes, Célia Margarida
Da Costa, Milton
author_role author
author2 Morais, Paula V
Alcobia Gomes, Célia Margarida
Da Costa, Milton
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório Comum
dc.contributor.author.fl_str_mv Ferreira, A.C.
Morais, Paula V
Alcobia Gomes, Célia Margarida
Da Costa, Milton
dc.subject.por.fl_str_mv Bacteria
Bacterial proteins
Fatty acids
Mineral waters
Reproducibility of results
Time factors
Electrophoresis, polyacrylamide gel
Numerical analysis, computer-assisted
Quinones
Bactérias
Proteínas de bactérias
Ácidos gordos
Águas minerais
Reprodutibilidade dos resultados
Eletroforese em gel de poliacrilamida
Análise numérica assistida por computador
Quinonas
Fatores de tempo
topic Bacteria
Bacterial proteins
Fatty acids
Mineral waters
Reproducibility of results
Time factors
Electrophoresis, polyacrylamide gel
Numerical analysis, computer-assisted
Quinones
Bactérias
Proteínas de bactérias
Ácidos gordos
Águas minerais
Reprodutibilidade dos resultados
Eletroforese em gel de poliacrilamida
Análise numérica assistida por computador
Quinonas
Fatores de tempo
description The microflora of a natural mineral water was studied immediately after bottling (T0) and after 7 d storage (T7) during 6 months, and isolates were clustered by SDS-PAGE of whole-cell protein profiles. Isolates from each cluster were further characterized by API 20NE, fatty acid composition and quinone profiles. The numerical analysis of the electrophoregrams of all bacteria isolated from the mineral water formed 15 clusters and five unclustered strains. Except for five minor clusters, all clusters were composed of strains isolated over several months. The numerical analysis of the electrophoregrams of bacteria isolated immediately after bottling formed 15 clusters while after 7 d storage only four of these populations could be isolated, indicating that populations present in the mineral water were stable and that changes occurring after bottling probably resulted from a selection process. Only one unclustered strain was identified simultaneously by all the systems, as Sphingomonas paucimobilis. The monitoring of the aquifer and the bottling system, and the construction of a large database with bacteria of the autochthonous flora allows the detection of alterations in the aquifer by changes in the microflora.
publishDate 1996
dc.date.none.fl_str_mv 1996-05-01T00:00:00Z
1996-05-01T00:00:00Z
2020-10-07T12:05:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.26/33490
url http://hdl.handle.net/10400.26/33490
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0021-8847
10.1111/j.1365-2672.1996.tb03246.x
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell
publisher.none.fl_str_mv Wiley Blackwell
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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