The extracellular proteins of Lactobacillus acidophilus DSM 20079T display anti-inflammatory effect in both in piglets, healthy human donors and Crohns Disease patients

• Autor(a) principal: Hidalgo-Cantabrana, Claudio
• Data de Publicação: 2020
• Outros Autores: Moro-García, Marco Antonio, Blanco-Míguez, Aitor, Fdez-Riverola, Florentino, Oliván, Mamen, Royo, Luis J., Riestra, Sabino, Margolles, Abelardo, Lourenço, Anália, Alonso-Arias, Rebeca, Sánchez, Borja
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/63827
• topic: Extracellular proteins; Tolerance; Anti-inflammatory; Treg; Innate immune system; Science & Technology

Lactobacillus genus includes both probiotic and representative strains of the human gut microbiota. Independent studies have reported on the anti-inflammatory properties of different Lactobacillus strains, although we are far from understanding the underlying molecular interplay. In this work we show that a daily administration of Lactobacillus acidophilus DSM20079T (DSM20079) to healthy piglets resulted in plasmatic increases of the anti-inflammatory IL10, whilst IL12 and the pro-inflammatory ratio IL12+TNF/IL10 decreased. The extracellular protein fraction of DSM20079 was identified as the responsible for the crosstalk interaction that elicited these tolerogenic effects. This strain was able to activate innate immune pathways in dendritic cells and to decrease the production of pro-inflammatory cytokines in both CD4+/CD8+ T cell subsets in healthy donors and in Crohns Disease patients. The tolerogenic effect exerted by the extracellular proteins of this strain suggests their potential use as coadjutant for therapeutic applications targeting chronic inflammatory illnesses.