In silico and functional analyses of immunomodulatory peptides encrypted in the human gut metaproteome
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/1822/65214 |
Resumo: | Supplementary data to this article can be found online at https:// doi.org/10.1016/j.jff.2020.103969. |
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In silico and functional analyses of immunomodulatory peptides encrypted in the human gut metaproteomeExtracellular proteinsToleranceAnti-inflammatoryTregInnate immune systemScience & TechnologySupplementary data to this article can be found online at https:// doi.org/10.1016/j.jff.2020.103969.This work supports the massive presence of potential immunomodulatory peptides in the human gut metaproteome. These peptides were identified through the MAHMI database as potentially anti-inflammatory, and sixteen of them synthesized for characterize their mechanism of action. From them, peptide HM14 was encrypted in an extracellular protein produced by Bifidobacterium longum, a common member of the human microbiota, and displayed the highest anti-inflammatory capability. Molecular mechanism of action of HM14 pointed to a specific interaction between this immunomodulatory peptide and antigen presenting cells, which resulted in a higher formation of iTreg cells. Moreover, HM14 was effective in decreasing pro-inflammatory parameters in PBMCs isolated from a cohort of Crohns patients. Finally, non-targeted metabolomics confirmed the ability of HM14 to modulate the metabolic activity of PBMCs to fulfil its energy and biosynthetic requirements. Overall, our combined in silico/multiomics approach supports the human gut metaproteome as a source for immunomodulatory peptides.Our work is supported by the Spanish “Programa Estatal de Investigación. Desarrollo e Innovación Orientada a los Retos de la Sociedad” (grants AGL2013-44761-P and AGL2016-78311-R); the Asociación Española Contra el Cancer (“Obtención de péptidos bioactivos contra el Cáncer Colo-Rectal a partir de secuencias genéticas de microbiomas intestinales”, Grant PS-2016), by the Asturias Regional Plan I + D + i for research groups (FYCYT-IDI/2018/000236) and by the Autonomic “Investigadores Emerxentes do Sistema Universitario de Galicia” (Grant EM2014/046). This work was partially supported by the Consellería de Educación. Universidades e Formación Profesional (Xunta de Galicia) under the scope of the strategic funding of ED431C2018/55-GRC Competitive Reference Group. Finally, the authors wish to thank Jaume Morales and Rubén García form Agilent Technologies for technical support.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoCambeiro-Pérez, NoeliaHidalgo-Cantabrana, ClaudioMoro-García, Marco AntonioBlanco-Míguez, AitorFdez-Riverola, FlorentinoRiestra, SabinoLourenço, AnáliaAlonso-Arias, RebecaMargolles, AbelardoMartínez-Carballo, ElenaSánchez, Borja2020-072020-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/65214engCambeiro-Pérez, Noelia; Hidalgo-Cantabrana, Claudio; Moro-García, Marco Antonio; Blanco-Míguez, Aitor; Fdez-Riverola, Florentino; Riestra, Sabino; Lourenço, Anália; Alonso-Arias, Rebeca; Margolles, Abelardo; Martínez-Carballo, Elena; Sánchez, Borja, In silico and functional analyses of immunomodulatory peptides encrypted in the human gut metaproteome. Journal of Functional Foods, 70(103969), 20201756464610.1016/j.jff.2020.103969https://www.journals.elsevier.com/journal-of-functional-foodsinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:00:20Zoai:repositorium.sdum.uminho.pt:1822/65214Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:50:12.698803Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
In silico and functional analyses of immunomodulatory peptides encrypted in the human gut metaproteome |
| title |
In silico and functional analyses of immunomodulatory peptides encrypted in the human gut metaproteome |
| spellingShingle |
In silico and functional analyses of immunomodulatory peptides encrypted in the human gut metaproteome Cambeiro-Pérez, Noelia Extracellular proteins Tolerance Anti-inflammatory Treg Innate immune system Science & Technology |
| title_short |
In silico and functional analyses of immunomodulatory peptides encrypted in the human gut metaproteome |
| title_full |
In silico and functional analyses of immunomodulatory peptides encrypted in the human gut metaproteome |
| title_fullStr |
In silico and functional analyses of immunomodulatory peptides encrypted in the human gut metaproteome |
| title_full_unstemmed |
In silico and functional analyses of immunomodulatory peptides encrypted in the human gut metaproteome |
| title_sort |
In silico and functional analyses of immunomodulatory peptides encrypted in the human gut metaproteome |
| author |
Cambeiro-Pérez, Noelia |
| author_facet |
Cambeiro-Pérez, Noelia Hidalgo-Cantabrana, Claudio Moro-García, Marco Antonio Blanco-Míguez, Aitor Fdez-Riverola, Florentino Riestra, Sabino Lourenço, Anália Alonso-Arias, Rebeca Margolles, Abelardo Martínez-Carballo, Elena Sánchez, Borja |
| author_role |
author |
| author2 |
Hidalgo-Cantabrana, Claudio Moro-García, Marco Antonio Blanco-Míguez, Aitor Fdez-Riverola, Florentino Riestra, Sabino Lourenço, Anália Alonso-Arias, Rebeca Margolles, Abelardo Martínez-Carballo, Elena Sánchez, Borja |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Cambeiro-Pérez, Noelia Hidalgo-Cantabrana, Claudio Moro-García, Marco Antonio Blanco-Míguez, Aitor Fdez-Riverola, Florentino Riestra, Sabino Lourenço, Anália Alonso-Arias, Rebeca Margolles, Abelardo Martínez-Carballo, Elena Sánchez, Borja |
| dc.subject.por.fl_str_mv |
Extracellular proteins Tolerance Anti-inflammatory Treg Innate immune system Science & Technology |
| topic |
Extracellular proteins Tolerance Anti-inflammatory Treg Innate immune system Science & Technology |
| description |
Supplementary data to this article can be found online at https:// doi.org/10.1016/j.jff.2020.103969. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-07 2020-07-01T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/1822/65214 |
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http://hdl.handle.net/1822/65214 |
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eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Cambeiro-Pérez, Noelia; Hidalgo-Cantabrana, Claudio; Moro-García, Marco Antonio; Blanco-Míguez, Aitor; Fdez-Riverola, Florentino; Riestra, Sabino; Lourenço, Anália; Alonso-Arias, Rebeca; Margolles, Abelardo; Martínez-Carballo, Elena; Sánchez, Borja, In silico and functional analyses of immunomodulatory peptides encrypted in the human gut metaproteome. Journal of Functional Foods, 70(103969), 2020 17564646 10.1016/j.jff.2020.103969 https://www.journals.elsevier.com/journal-of-functional-foods |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
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Elsevier |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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