Artefacts induced on c-type haem proteins by electrode surfaces

Detalhes bibliográficos
Autor(a) principal: Sousa, Patrícia M. Paes de
Data de Publicação: 2011
Outros Autores: Pauleta, Sofia R., Gonçalves, M. Lurdes Simões, Pettigrew, Graham W., Moura, Isabel, Moura, José J. G., Santos, Margarida M. Correia dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/8622
Resumo: J Biol Inorg Chem (2011) 16:209–215 DOI 10.1007/s00775-010-0717-z
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spelling Artefacts induced on c-type haem proteins by electrode surfacesArtefactsElectrochemistryc-type haem proteinsPeroxidasesPyrolytic graphite electrodesJ Biol Inorg Chem (2011) 16:209–215 DOI 10.1007/s00775-010-0717-zIn this work it is demonstrated that the characterization of c-type haem containing proteins by electrochemical techniques needs to be cautiously performed when using pyrolytic graphite electrodes. An altered form of the cytochromes, which has a redox potential 300 mV lower than that of the native state and displays peroxidatic activity, can be induced by interaction with the pyrolytic graphite electrode. Proper control experiments need to be performed, as altered conformations of the enzymes containing c-type haems can show activity towards the enzyme substrate. The work was focused on the study of the activation mechanism and catalytic activity of cytochrome c peroxidase from Paracoccus pantotrophus. The results could only be interpreted with the assignment of the observed non-turnover and catalytic signals to a non-native conformation state of the electron-transferring haem. The same phenomenon was detected for Met–His monohaem cytochromes (mitochondrial cytochrome c and Desulfovibrio vulgaris cytochrome c-553), as well as for the bis-His multihaem cytochrome c 3 from Desulfovibrio gigas, showing that this effect is independent of the axial coordination of the c-type haem protein. Thus, the interpretation of electrochemical signals of c-type (multi)haem proteins at pyrolytic graphite electrodes must be carefully performed, to avoid misassignment of the signals and incorrect interpretation of catalytic intermediates.SpringerRUNSousa, Patrícia M. Paes dePauleta, Sofia R.Gonçalves, M. Lurdes SimõesPettigrew, Graham W.Moura, IsabelMoura, José J. G.Santos, Margarida M. Correia dos2013-01-30T12:10:25Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/8622eng1432-1327info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:12:19Zoai:run.unl.pt:10362/8622Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:12:19Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Artefacts induced on c-type haem proteins by electrode surfaces
title Artefacts induced on c-type haem proteins by electrode surfaces
spellingShingle Artefacts induced on c-type haem proteins by electrode surfaces
Sousa, Patrícia M. Paes de
Artefacts
Electrochemistry
c-type haem proteins
Peroxidases
Pyrolytic graphite electrodes
title_short Artefacts induced on c-type haem proteins by electrode surfaces
title_full Artefacts induced on c-type haem proteins by electrode surfaces
title_fullStr Artefacts induced on c-type haem proteins by electrode surfaces
title_full_unstemmed Artefacts induced on c-type haem proteins by electrode surfaces
title_sort Artefacts induced on c-type haem proteins by electrode surfaces
author Sousa, Patrícia M. Paes de
author_facet Sousa, Patrícia M. Paes de
Pauleta, Sofia R.
Gonçalves, M. Lurdes Simões
Pettigrew, Graham W.
Moura, Isabel
Moura, José J. G.
Santos, Margarida M. Correia dos
author_role author
author2 Pauleta, Sofia R.
Gonçalves, M. Lurdes Simões
Pettigrew, Graham W.
Moura, Isabel
Moura, José J. G.
Santos, Margarida M. Correia dos
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Sousa, Patrícia M. Paes de
Pauleta, Sofia R.
Gonçalves, M. Lurdes Simões
Pettigrew, Graham W.
Moura, Isabel
Moura, José J. G.
Santos, Margarida M. Correia dos
dc.subject.por.fl_str_mv Artefacts
Electrochemistry
c-type haem proteins
Peroxidases
Pyrolytic graphite electrodes
topic Artefacts
Electrochemistry
c-type haem proteins
Peroxidases
Pyrolytic graphite electrodes
description J Biol Inorg Chem (2011) 16:209–215 DOI 10.1007/s00775-010-0717-z
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
2013-01-30T12:10:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/8622
url http://hdl.handle.net/10362/8622
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1432-1327
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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