Host-induced changes in the cell surface N-linked glycoproteins, from Aspergillus fumigatus. Search for specific targets with potential for clinical therapy and/or diagnosis.

Detalhes bibliográficos
Autor(a) principal: Garcia, Sofia Matos Flores Évora
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/8194
Resumo: This dissertation is presented to obtain a Master degree in Structural and Functional Biochemistry
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spelling Host-induced changes in the cell surface N-linked glycoproteins, from Aspergillus fumigatus. Search for specific targets with potential for clinical therapy and/or diagnosis.Aspergillus fumigatusExoglycomeLectinGlycoproteinsN-linked oligosaccharidesThis dissertation is presented to obtain a Master degree in Structural and Functional BiochemistryThe fungus Aspergillus fumigatus is responsible for causing invasive aspergillosis in human lungs, a fatal disease in immunocompromised patients. The development of such diseases is typically associated with a deficient immune response in the host as well as with phenotypic changes at cellular level of the fungus itself. The sequencing of the fungal genome has allowed the study of the proteome and its constituents, making it possible to explain the reason to such changes. The collection of carbohydrate moieties present in N- and O-linked glycoproteins and glycolipids, which protrude outwards from the cell membrane, has been defined as the exoglycome. Furthermore, studies have demonstrated that changes suffered by the exoglycome of A. fumigatus are the main cause of the fungus infectious potential. Therefore, identification and characterization of the different carbohydrate structures that comprise the fungal exoglycome has become of great importance in order to increase the knowledge of the fungus pathogenicity. In this study, several experimental techniques developed in proteomics and glycomics areas were used in an attempt to identify the main components of the cell membrane proteome of A. fumigatus as well as the N-linked oligosaccharides structure that comprise the fungal exoglycome. Two methods for glycoprotein detection were used that are based in the non-covalent binding of lectins to specific oligosaccharides and the oxidation of carbohydrate groups followed by conjugation with a chromogenic or tagged substrate. In an attempt to identify the glycoproteins that comprise the proteome was performed, mass spectrometry was used, however the results were inconclusive. Certain factors, such as lack of homology between the sequenced peptides from membrane proteins with protein sequences already described in the databases were evaluated and questioned, but with no conclusive answers.Faculdade de Ciências e TecnologiaMonteiro, SaraFreitas, ReginaRUNGarcia, Sofia Matos Flores Évora2012-11-23T11:51:02Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/8194enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:11:48Zoai:run.unl.pt:10362/8194Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:11:48Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Host-induced changes in the cell surface N-linked glycoproteins, from Aspergillus fumigatus. Search for specific targets with potential for clinical therapy and/or diagnosis.
title Host-induced changes in the cell surface N-linked glycoproteins, from Aspergillus fumigatus. Search for specific targets with potential for clinical therapy and/or diagnosis.
spellingShingle Host-induced changes in the cell surface N-linked glycoproteins, from Aspergillus fumigatus. Search for specific targets with potential for clinical therapy and/or diagnosis.
Garcia, Sofia Matos Flores Évora
Aspergillus fumigatus
Exoglycome
Lectin
Glycoproteins
N-linked oligosaccharides
title_short Host-induced changes in the cell surface N-linked glycoproteins, from Aspergillus fumigatus. Search for specific targets with potential for clinical therapy and/or diagnosis.
title_full Host-induced changes in the cell surface N-linked glycoproteins, from Aspergillus fumigatus. Search for specific targets with potential for clinical therapy and/or diagnosis.
title_fullStr Host-induced changes in the cell surface N-linked glycoproteins, from Aspergillus fumigatus. Search for specific targets with potential for clinical therapy and/or diagnosis.
title_full_unstemmed Host-induced changes in the cell surface N-linked glycoproteins, from Aspergillus fumigatus. Search for specific targets with potential for clinical therapy and/or diagnosis.
title_sort Host-induced changes in the cell surface N-linked glycoproteins, from Aspergillus fumigatus. Search for specific targets with potential for clinical therapy and/or diagnosis.
author Garcia, Sofia Matos Flores Évora
author_facet Garcia, Sofia Matos Flores Évora
author_role author
dc.contributor.none.fl_str_mv Monteiro, Sara
Freitas, Regina
RUN
dc.contributor.author.fl_str_mv Garcia, Sofia Matos Flores Évora
dc.subject.por.fl_str_mv Aspergillus fumigatus
Exoglycome
Lectin
Glycoproteins
N-linked oligosaccharides
topic Aspergillus fumigatus
Exoglycome
Lectin
Glycoproteins
N-linked oligosaccharides
description This dissertation is presented to obtain a Master degree in Structural and Functional Biochemistry
publishDate 2012
dc.date.none.fl_str_mv 2012-11-23T11:51:02Z
2012
2012-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/8194
url http://hdl.handle.net/10362/8194
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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