Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.8/6658 |
Resumo: | Increasing evidence points to inflammation as a key factor in the pathogenesis of diabetic retinopathy (DR). Choroidal inflammatory changes in diabetes have been reported and in vivo choroidal thickness (CT) has been searched as a marker of retinopathy with contradictory results. We aimed to investigate the early stages in the retina and choroid in an animal model of Type 1 diabetes. Type 1 diabetes was induced in male Wistar rats via a single i.p. streptozotocin injection. At 8 weeks after disease onset, CT, choroidal vascular density, VEGF and VEGFR2 expression, microglial cell and pericyte distribution were evaluated. Diabetic rats showed no significant change in CT and choroidal vascular density. A widened pericyte-free gap between the retinal pigment epithelium and the choroid was observed in diabetic rats. The immunoreactivity of VEGFR2 was decreased in the retina of diabetic rats, despite no statistically significant difference in the immunoreactivity of VEGF. The density of microglial cells significantly increased in the choroid and retina of diabetic rats. Reactive microglial cells were found to be more abundant in the choroid of diabetic rats. Evidences of the interconnection between the superficial, intermediate, and deep plexuses of the retina were also observed. At early stages, Type 1 diabetes does not affect choroidal thickness and choroidal vascular density. Proliferation and reactivity of microglial cells occurs in the choroidal stroma and the retina. The expression of VEGFR2 decreases in the retina. |
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Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetesChoroidRetinaType 1 diabetesChoroidal thicknessMicrogliaIncreasing evidence points to inflammation as a key factor in the pathogenesis of diabetic retinopathy (DR). Choroidal inflammatory changes in diabetes have been reported and in vivo choroidal thickness (CT) has been searched as a marker of retinopathy with contradictory results. We aimed to investigate the early stages in the retina and choroid in an animal model of Type 1 diabetes. Type 1 diabetes was induced in male Wistar rats via a single i.p. streptozotocin injection. At 8 weeks after disease onset, CT, choroidal vascular density, VEGF and VEGFR2 expression, microglial cell and pericyte distribution were evaluated. Diabetic rats showed no significant change in CT and choroidal vascular density. A widened pericyte-free gap between the retinal pigment epithelium and the choroid was observed in diabetic rats. The immunoreactivity of VEGFR2 was decreased in the retina of diabetic rats, despite no statistically significant difference in the immunoreactivity of VEGF. The density of microglial cells significantly increased in the choroid and retina of diabetic rats. Reactive microglial cells were found to be more abundant in the choroid of diabetic rats. Evidences of the interconnection between the superficial, intermediate, and deep plexuses of the retina were also observed. At early stages, Type 1 diabetes does not affect choroidal thickness and choroidal vascular density. Proliferation and reactivity of microglial cells occurs in the choroidal stroma and the retina. The expression of VEGFR2 decreases in the retina.ElsevierIC-OnlineCampos, AntónioCampos, Elisa J.Martins, JoãoRodrigues, Flávia S.C.Silva, RufinoAmbrósio, António Francisco2022-02-11T14:51:49Z2020-08-222020-08-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/6658engCampos, A., Campos, E. J., Martins, J., Rodrigues, F. S. C., Silva, R., & Ambrósio, A. F. (2020). Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes. Experimental Eye Research, 199, 108195. https://doi.org/10.1016/j.exer.2020.1081950014-483510.1016/j.exer.2020.108195metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:53:23Zoai:iconline.ipleiria.pt:10400.8/6658Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:49:45.862836Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes |
title |
Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes |
spellingShingle |
Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes Campos, António Choroid Retina Type 1 diabetes Choroidal thickness Microglia |
title_short |
Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes |
title_full |
Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes |
title_fullStr |
Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes |
title_full_unstemmed |
Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes |
title_sort |
Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes |
author |
Campos, António |
author_facet |
Campos, António Campos, Elisa J. Martins, João Rodrigues, Flávia S.C. Silva, Rufino Ambrósio, António Francisco |
author_role |
author |
author2 |
Campos, Elisa J. Martins, João Rodrigues, Flávia S.C. Silva, Rufino Ambrósio, António Francisco |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
IC-Online |
dc.contributor.author.fl_str_mv |
Campos, António Campos, Elisa J. Martins, João Rodrigues, Flávia S.C. Silva, Rufino Ambrósio, António Francisco |
dc.subject.por.fl_str_mv |
Choroid Retina Type 1 diabetes Choroidal thickness Microglia |
topic |
Choroid Retina Type 1 diabetes Choroidal thickness Microglia |
description |
Increasing evidence points to inflammation as a key factor in the pathogenesis of diabetic retinopathy (DR). Choroidal inflammatory changes in diabetes have been reported and in vivo choroidal thickness (CT) has been searched as a marker of retinopathy with contradictory results. We aimed to investigate the early stages in the retina and choroid in an animal model of Type 1 diabetes. Type 1 diabetes was induced in male Wistar rats via a single i.p. streptozotocin injection. At 8 weeks after disease onset, CT, choroidal vascular density, VEGF and VEGFR2 expression, microglial cell and pericyte distribution were evaluated. Diabetic rats showed no significant change in CT and choroidal vascular density. A widened pericyte-free gap between the retinal pigment epithelium and the choroid was observed in diabetic rats. The immunoreactivity of VEGFR2 was decreased in the retina of diabetic rats, despite no statistically significant difference in the immunoreactivity of VEGF. The density of microglial cells significantly increased in the choroid and retina of diabetic rats. Reactive microglial cells were found to be more abundant in the choroid of diabetic rats. Evidences of the interconnection between the superficial, intermediate, and deep plexuses of the retina were also observed. At early stages, Type 1 diabetes does not affect choroidal thickness and choroidal vascular density. Proliferation and reactivity of microglial cells occurs in the choroidal stroma and the retina. The expression of VEGFR2 decreases in the retina. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-22 2020-08-22T00:00:00Z 2022-02-11T14:51:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.8/6658 |
url |
http://hdl.handle.net/10400.8/6658 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Campos, A., Campos, E. J., Martins, J., Rodrigues, F. S. C., Silva, R., & Ambrósio, A. F. (2020). Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes. Experimental Eye Research, 199, 108195. https://doi.org/10.1016/j.exer.2020.108195 0014-4835 10.1016/j.exer.2020.108195 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
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metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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