Induced pluripotent stem cell-derived vascular networks to screen nano-bio interactions

Detalhes bibliográficos
Autor(a) principal: Estronca, Luís
Data de Publicação: 2021
Outros Autores: Francisco, Vitor, Pitrez, Patrícia, Honório, Inês, Carvalho, Lara, Vazão, Helena, Blersch, Josephine, Rai, Akhilesh, Nissan, Xavier, Simon, Ulrich, Grãos, Mário, Saúde, Leonor, Ferreira, Lino
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/46322
Resumo: © The Royal Society of Chemistry, 2021. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)
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spelling Induced pluripotent stem cell-derived vascular networks to screen nano-bio interactions© The Royal Society of Chemistry, 2021. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)The vascular bioactivity/safety of nanomaterials is typically evaluated by animal testing, which is of low throughput and does not account for biological differences between animals and humans such as ageing, metabolism and disease profiles. The development of personalized human in vitro platforms to evaluate the interaction of nanomaterials with the vascular system would be important for both therapeutic and regenerative medicine. A library of 30 nanoparticle (NP) formulations, in use in imaging, antimicrobial and pharmaceutical applications, was evaluated in a reporter zebrafish model of vasculogenesis and then tested in personalized humanized models composed of human-induced pluripotent stem cell (hiPSC)-derived endothelial cells (ECs) with ‘‘young’’ and ‘‘aged’’ phenotypes in 3 vascular network formats: 2D (in polystyrene dish), 3D (in Matrigel) and in a blood vessel on a chip. As a proof of concept, vascular toxicity was used as the main readout. The results show that the toxicity profile of NPs to hiPSC-ECs was dependent on the ‘‘age’’ of the endothelial cells and vascular network format. hiPSC-ECs were less susceptible to the cytotoxicity effect of NPs when cultured in flow than in static conditions, the protective effect being mediated, at least in part, by glycocalyx. Overall, the results presented here highlight the relevance of in vitro hiPSC-derived vascular systems to screen vascular nanomaterial interactions.This work was funded by FEDER through the Program COMPETE and by Portuguese fund through FCT in the context of the projects PTDC/SAU-TOX/121887/2010, POCI-01-0145-FEDER-029229, POCI-01-0145-FEDER-016390 and POCI-01-0145-FEDER-029414, as well as the European project ERAatUC (ref. 669088). PP wishes to thank FCT for a BD fellowship (SFRH/BD/71042/2010). The iMM Fish Facility was supported by CONGENTO LISBOA-01-0145-FEDER-022170, co-financed by FCT (Portugal) and Lisboa 2020, under the PORTUGAL 2020 agreement (European Regional Development Fund). The authors have no conflict of interest to disclose.Royal Society of ChemistryRepositório da Universidade de LisboaEstronca, LuísFrancisco, VitorPitrez, PatríciaHonório, InêsCarvalho, LaraVazão, HelenaBlersch, JosephineRai, AkhileshNissan, XavierSimon, UlrichGrãos, MárioSaúde, LeonorFerreira, Lino2021-02-15T13:23:11Z2021-02-122021-02-12T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/46322engNanoscale Horiz. 2021 Feb 1210.1039/d0nh00550a2055-6764info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:48:34Zoai:repositorio.ul.pt:10451/46322Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:58:32.815577Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Induced pluripotent stem cell-derived vascular networks to screen nano-bio interactions
title Induced pluripotent stem cell-derived vascular networks to screen nano-bio interactions
spellingShingle Induced pluripotent stem cell-derived vascular networks to screen nano-bio interactions
Estronca, Luís
title_short Induced pluripotent stem cell-derived vascular networks to screen nano-bio interactions
title_full Induced pluripotent stem cell-derived vascular networks to screen nano-bio interactions
title_fullStr Induced pluripotent stem cell-derived vascular networks to screen nano-bio interactions
title_full_unstemmed Induced pluripotent stem cell-derived vascular networks to screen nano-bio interactions
title_sort Induced pluripotent stem cell-derived vascular networks to screen nano-bio interactions
author Estronca, Luís
author_facet Estronca, Luís
Francisco, Vitor
Pitrez, Patrícia
Honório, Inês
Carvalho, Lara
Vazão, Helena
Blersch, Josephine
Rai, Akhilesh
Nissan, Xavier
Simon, Ulrich
Grãos, Mário
Saúde, Leonor
Ferreira, Lino
author_role author
author2 Francisco, Vitor
Pitrez, Patrícia
Honório, Inês
Carvalho, Lara
Vazão, Helena
Blersch, Josephine
Rai, Akhilesh
Nissan, Xavier
Simon, Ulrich
Grãos, Mário
Saúde, Leonor
Ferreira, Lino
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Estronca, Luís
Francisco, Vitor
Pitrez, Patrícia
Honório, Inês
Carvalho, Lara
Vazão, Helena
Blersch, Josephine
Rai, Akhilesh
Nissan, Xavier
Simon, Ulrich
Grãos, Mário
Saúde, Leonor
Ferreira, Lino
description © The Royal Society of Chemistry, 2021. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)
publishDate 2021
dc.date.none.fl_str_mv 2021-02-15T13:23:11Z
2021-02-12
2021-02-12T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/46322
url http://hdl.handle.net/10451/46322
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nanoscale Horiz. 2021 Feb 12
10.1039/d0nh00550a
2055-6764
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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