Determination of neutrophil Fcc receptor IIIb antigens (HNA-1a, HNA-1b and HNA-1c) by fluorescence-primed allele-specific polymerase chain reaction

Detalhes bibliográficos
Autor(a) principal: Costa, Elísio
Data de Publicação: 2004
Outros Autores: Antunes, Marika Bini, Faria, Susana, Vieira, Emília, Branca, Rosa, Barbot, José, Santos, Rosário
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/507
Resumo: We evaluate a technique for genotyping HNA-1a, -1b and -1c antigens, resorting to fluorescence-primed allele-specific polymerase chain reaction (FPAS-PCR), and determine the frequency of the different genotypes in a normal Portuguese population. Our results indicate that the FPAS-PCR system is a reliable and simple tool for genotyping the neutrophil Fcc receptor IIIB antigens. The HNA-1a, -1b and -1c gene frequencies of 42.98, 84.21 and 6.14%, respectively, found in this study are similar to those reported for other white populations.
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spelling Determination of neutrophil Fcc receptor IIIb antigens (HNA-1a, HNA-1b and HNA-1c) by fluorescence-primed allele-specific polymerase chain reactionFccRIIIB antigensNeutrophilWe evaluate a technique for genotyping HNA-1a, -1b and -1c antigens, resorting to fluorescence-primed allele-specific polymerase chain reaction (FPAS-PCR), and determine the frequency of the different genotypes in a normal Portuguese population. Our results indicate that the FPAS-PCR system is a reliable and simple tool for genotyping the neutrophil Fcc receptor IIIB antigens. The HNA-1a, -1b and -1c gene frequencies of 42.98, 84.21 and 6.14%, respectively, found in this study are similar to those reported for other white populations.Biblioteca Digital do IPBCosta, ElísioAntunes, Marika BiniFaria, SusanaVieira, EmíliaBranca, RosaBarbot, JoséSantos, Rosário2008-02-21T10:12:03Z20042004-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/507engCosta, Elísio; Antunes, Marika Bini; Faria, Susana; Vieira, Emília; Branca, Rosa; Barbot, José; Santos, Rosário (2004). Determination of neutrophil Fcc receptor IIIb antigens (HNA-1a, HNA-1b and HNA-1c) by fluorescence-primed allele-specific polymerase chain reaction. Clinical and Laboratory Haematology. ISSN 1365-2257. 26:5, p. 319–3211365-2257info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:03:28Zoai:bibliotecadigital.ipb.pt:10198/507Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:54:17.281155Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Determination of neutrophil Fcc receptor IIIb antigens (HNA-1a, HNA-1b and HNA-1c) by fluorescence-primed allele-specific polymerase chain reaction
title Determination of neutrophil Fcc receptor IIIb antigens (HNA-1a, HNA-1b and HNA-1c) by fluorescence-primed allele-specific polymerase chain reaction
spellingShingle Determination of neutrophil Fcc receptor IIIb antigens (HNA-1a, HNA-1b and HNA-1c) by fluorescence-primed allele-specific polymerase chain reaction
Costa, Elísio
FccRIIIB antigens
Neutrophil
title_short Determination of neutrophil Fcc receptor IIIb antigens (HNA-1a, HNA-1b and HNA-1c) by fluorescence-primed allele-specific polymerase chain reaction
title_full Determination of neutrophil Fcc receptor IIIb antigens (HNA-1a, HNA-1b and HNA-1c) by fluorescence-primed allele-specific polymerase chain reaction
title_fullStr Determination of neutrophil Fcc receptor IIIb antigens (HNA-1a, HNA-1b and HNA-1c) by fluorescence-primed allele-specific polymerase chain reaction
title_full_unstemmed Determination of neutrophil Fcc receptor IIIb antigens (HNA-1a, HNA-1b and HNA-1c) by fluorescence-primed allele-specific polymerase chain reaction
title_sort Determination of neutrophil Fcc receptor IIIb antigens (HNA-1a, HNA-1b and HNA-1c) by fluorescence-primed allele-specific polymerase chain reaction
author Costa, Elísio
author_facet Costa, Elísio
Antunes, Marika Bini
Faria, Susana
Vieira, Emília
Branca, Rosa
Barbot, José
Santos, Rosário
author_role author
author2 Antunes, Marika Bini
Faria, Susana
Vieira, Emília
Branca, Rosa
Barbot, José
Santos, Rosário
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Costa, Elísio
Antunes, Marika Bini
Faria, Susana
Vieira, Emília
Branca, Rosa
Barbot, José
Santos, Rosário
dc.subject.por.fl_str_mv FccRIIIB antigens
Neutrophil
topic FccRIIIB antigens
Neutrophil
description We evaluate a technique for genotyping HNA-1a, -1b and -1c antigens, resorting to fluorescence-primed allele-specific polymerase chain reaction (FPAS-PCR), and determine the frequency of the different genotypes in a normal Portuguese population. Our results indicate that the FPAS-PCR system is a reliable and simple tool for genotyping the neutrophil Fcc receptor IIIB antigens. The HNA-1a, -1b and -1c gene frequencies of 42.98, 84.21 and 6.14%, respectively, found in this study are similar to those reported for other white populations.
publishDate 2004
dc.date.none.fl_str_mv 2004
2004-01-01T00:00:00Z
2008-02-21T10:12:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/507
url http://hdl.handle.net/10198/507
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Costa, Elísio; Antunes, Marika Bini; Faria, Susana; Vieira, Emília; Branca, Rosa; Barbot, José; Santos, Rosário (2004). Determination of neutrophil Fcc receptor IIIb antigens (HNA-1a, HNA-1b and HNA-1c) by fluorescence-primed allele-specific polymerase chain reaction. Clinical and Laboratory Haematology. ISSN 1365-2257. 26:5, p. 319–321
1365-2257
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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