The structure of a Plasmodium vivax Tryptophan Rich Antigen domain suggests a lipid binding function for a pan-Plasmodium multi-gene family

Detalhes bibliográficos
Autor(a) principal: Kundu, Prasun
Data de Publicação: 2023
Outros Autores: Naskar, Deboki, McKie, Shannon J, Dass, Sheena, Kanjee, Usheer, Introini, Viola, Ferreira, Marcelo U, Cicuta, Pietro, Duraisingh, Manoj, Deane, Janet E, Rayner, Julian C
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/160091
Resumo: © 2023. Springer Nature Limited.
id RCAP_0abef9b10f131b499f7b4fdafb768af0
oai_identifier_str oai:run.unl.pt:10362/160091
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling The structure of a Plasmodium vivax Tryptophan Rich Antigen domain suggests a lipid binding function for a pan-Plasmodium multi-gene familyHumansPlasmodium vivax/geneticsTryptophanPlasmodiumAntigens, Protozoan/geneticsMalaria, VivaxSulfoglycosphingolipidsQR MicrobiologyQD ChemistryQR180 ImmunologyParasitologyInfectious DiseasesBiochemistry, Genetics and Molecular Biology(all)SDG 3 - Good Health and Well-being© 2023. Springer Nature Limited.Tryptophan Rich Antigens (TRAgs) are encoded by a multi-gene family found in all Plasmodium species, but are significantly expanded in P. vivax and closely related parasites. We show that multiple P. vivax TRAgs are expressed on the merozoite surface and that one, PVP01_0000100 binds red blood cells with a strong preference for reticulocytes. Using X-ray crystallography, we solved the structure of the PVP01_0000100 C-terminal tryptophan rich domain, which defines the TRAg family, revealing a three-helical bundle that is conserved across Plasmodium and has structural homology with lipid-binding BAR domains involved in membrane remodelling. Biochemical assays confirm that the PVP01_0000100 C-terminal domain has lipid binding activity with preference for sulfatide, a glycosphingolipid present in the outer leaflet of plasma membranes. Deletion of the putative orthologue in P. knowlesi, PKNH_1300500, impacts invasion in reticulocytes, suggesting a role during this essential process. Together, this work defines an emerging molecular function for the Plasmodium TRAg family.Individual Health Care (IHC)Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)RUNKundu, PrasunNaskar, DebokiMcKie, Shannon JDass, SheenaKanjee, UsheerIntroini, ViolaFerreira, Marcelo UCicuta, PietroDuraisingh, ManojDeane, Janet ERayner, Julian C2023-11-17T22:11:14Z2023-09-142023-09-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/160091eng2041-1723PURE: 72339375https://doi.org/10.1038/s41467-023-40885-8info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:42:40Zoai:run.unl.pt:10362/160091Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:51.870702Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The structure of a Plasmodium vivax Tryptophan Rich Antigen domain suggests a lipid binding function for a pan-Plasmodium multi-gene family
title The structure of a Plasmodium vivax Tryptophan Rich Antigen domain suggests a lipid binding function for a pan-Plasmodium multi-gene family
spellingShingle The structure of a Plasmodium vivax Tryptophan Rich Antigen domain suggests a lipid binding function for a pan-Plasmodium multi-gene family
Kundu, Prasun
Humans
Plasmodium vivax/genetics
Tryptophan
Plasmodium
Antigens, Protozoan/genetics
Malaria, Vivax
Sulfoglycosphingolipids
QR Microbiology
QD Chemistry
QR180 Immunology
Parasitology
Infectious Diseases
Biochemistry, Genetics and Molecular Biology(all)
SDG 3 - Good Health and Well-being
title_short The structure of a Plasmodium vivax Tryptophan Rich Antigen domain suggests a lipid binding function for a pan-Plasmodium multi-gene family
title_full The structure of a Plasmodium vivax Tryptophan Rich Antigen domain suggests a lipid binding function for a pan-Plasmodium multi-gene family
title_fullStr The structure of a Plasmodium vivax Tryptophan Rich Antigen domain suggests a lipid binding function for a pan-Plasmodium multi-gene family
title_full_unstemmed The structure of a Plasmodium vivax Tryptophan Rich Antigen domain suggests a lipid binding function for a pan-Plasmodium multi-gene family
title_sort The structure of a Plasmodium vivax Tryptophan Rich Antigen domain suggests a lipid binding function for a pan-Plasmodium multi-gene family
author Kundu, Prasun
author_facet Kundu, Prasun
Naskar, Deboki
McKie, Shannon J
Dass, Sheena
Kanjee, Usheer
Introini, Viola
Ferreira, Marcelo U
Cicuta, Pietro
Duraisingh, Manoj
Deane, Janet E
Rayner, Julian C
author_role author
author2 Naskar, Deboki
McKie, Shannon J
Dass, Sheena
Kanjee, Usheer
Introini, Viola
Ferreira, Marcelo U
Cicuta, Pietro
Duraisingh, Manoj
Deane, Janet E
Rayner, Julian C
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Individual Health Care (IHC)
Global Health and Tropical Medicine (GHTM)
Instituto de Higiene e Medicina Tropical (IHMT)
RUN
dc.contributor.author.fl_str_mv Kundu, Prasun
Naskar, Deboki
McKie, Shannon J
Dass, Sheena
Kanjee, Usheer
Introini, Viola
Ferreira, Marcelo U
Cicuta, Pietro
Duraisingh, Manoj
Deane, Janet E
Rayner, Julian C
dc.subject.por.fl_str_mv Humans
Plasmodium vivax/genetics
Tryptophan
Plasmodium
Antigens, Protozoan/genetics
Malaria, Vivax
Sulfoglycosphingolipids
QR Microbiology
QD Chemistry
QR180 Immunology
Parasitology
Infectious Diseases
Biochemistry, Genetics and Molecular Biology(all)
SDG 3 - Good Health and Well-being
topic Humans
Plasmodium vivax/genetics
Tryptophan
Plasmodium
Antigens, Protozoan/genetics
Malaria, Vivax
Sulfoglycosphingolipids
QR Microbiology
QD Chemistry
QR180 Immunology
Parasitology
Infectious Diseases
Biochemistry, Genetics and Molecular Biology(all)
SDG 3 - Good Health and Well-being
description © 2023. Springer Nature Limited.
publishDate 2023
dc.date.none.fl_str_mv 2023-11-17T22:11:14Z
2023-09-14
2023-09-14T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/160091
url http://hdl.handle.net/10362/160091
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2041-1723
PURE: 72339375
https://doi.org/10.1038/s41467-023-40885-8
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138160856268800

Registros relacionados