Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/57875 |
Resumo: | Bovine tuberculosis is a zoonotic disease with largely unknown impact in Africa, with risk factors such as HIV and direct contact with animals or consumption of Mycobacterium bovis infected animal products. In order to understand and quantify this risk and design intervention strategies, good epidemiological studies are needed. Such studies can include molecular typing of M. bovis isolates. The aim of this study was to apply these tools to provide novel information concerning the distribution of bovine tuberculosis in cattle in Mozambique and thereby provide relevant information to guide policy development and strategies to contain the disease in livestock, and reduce the risk associated with transmission to humans. A collection of 178 M. bovis isolates was obtained from cattle in Mozambique. Using spoligotyping and regions of difference analysis, we classified the isolates into clonal complexes, thus reporting the first characterisation of M. bovis strains in this region. Data from MIRU-VNTR typing was used to compare isolates from a number of African countries, revealing a deeply geographically structured diversity of M. bovis. Eastern Africa appears to show high diversity, suggesting deep evolution in that region. The diversity of M. bovis in Africa does not seem to be a function of recent importation of animals, but is probably maintained within each particular region by constant reinfection from reservoir animals. Understanding the transmission routes of M. bovis in Mozambique and elsewhere is essential in order to focus public health and veterinary resources to contain bovine tuberculosis. |
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Genetic diversity and potential routes of transmission of Mycobacterium bovis in MozambiqueAnimalsCattleGenotypeMolecular EpidemiologyMolecular TypingMozambiqueMycobacterium bovisTuberculosis, BovineZoonosesDisease Transmission, InfectiousGenetic VariationCiências Médicas::Medicina BásicaScience & TechnologyBovine tuberculosis is a zoonotic disease with largely unknown impact in Africa, with risk factors such as HIV and direct contact with animals or consumption of Mycobacterium bovis infected animal products. In order to understand and quantify this risk and design intervention strategies, good epidemiological studies are needed. Such studies can include molecular typing of M. bovis isolates. The aim of this study was to apply these tools to provide novel information concerning the distribution of bovine tuberculosis in cattle in Mozambique and thereby provide relevant information to guide policy development and strategies to contain the disease in livestock, and reduce the risk associated with transmission to humans. A collection of 178 M. bovis isolates was obtained from cattle in Mozambique. Using spoligotyping and regions of difference analysis, we classified the isolates into clonal complexes, thus reporting the first characterisation of M. bovis strains in this region. Data from MIRU-VNTR typing was used to compare isolates from a number of African countries, revealing a deeply geographically structured diversity of M. bovis. Eastern Africa appears to show high diversity, suggesting deep evolution in that region. The diversity of M. bovis in Africa does not seem to be a function of recent importation of animals, but is probably maintained within each particular region by constant reinfection from reservoir animals. Understanding the transmission routes of M. bovis in Mozambique and elsewhere is essential in order to focus public health and veterinary resources to contain bovine tuberculosis.This work was supported by the Swedish International Development Cooperation Agency through the Eduardo Mondlane University and Karolinska Institutet Research and Training collaboration. TR and MCN acknowledge the project NORTE-01-0145-FEDER-000013, supported by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement. TR is supported by the Portuguese Foundation for Science and Technology (FCT) through a postdoctoral grant (SFRH/BPD/108126/2015). This work was partly supported by the South African Medical Research Council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.info:eu-repo/semantics/publishedVersionPublic Library of Science (PLOS)Universidade do MinhoMachado, AdelinaRito, TeresaGhebremichael, SolomonMuhate, NuelmaMaxhuza, GabrielMacuamule, CustodiaMoiane, IvaniaMacucule, BaltazarMarranangumbe, Angelica SuzanaBaptista, JorgeManguele, JoaquimKoivula, TuijaStreicher, Elizabeth MariaWarren, Robin MarkKallenius, Gunillavan Helden, PaulCorreia-Neves, M2018-012018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/57875engMachado, A., Rito, T., Ghebremichael, S., Muhate, N., et. al.(2018). Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique. PLoS neglected tropical diseases, 12(1), e00061471935-27271935-273510.1371/journal.pntd.000614729346413https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0006147info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:00:48Zoai:repositorium.sdum.uminho.pt:1822/57875Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:50:40.435300Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique |
title |
Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique |
spellingShingle |
Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique Machado, Adelina Animals Cattle Genotype Molecular Epidemiology Molecular Typing Mozambique Mycobacterium bovis Tuberculosis, Bovine Zoonoses Disease Transmission, Infectious Genetic Variation Ciências Médicas::Medicina Básica Science & Technology |
title_short |
Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique |
title_full |
Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique |
title_fullStr |
Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique |
title_full_unstemmed |
Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique |
title_sort |
Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique |
author |
Machado, Adelina |
author_facet |
Machado, Adelina Rito, Teresa Ghebremichael, Solomon Muhate, Nuelma Maxhuza, Gabriel Macuamule, Custodia Moiane, Ivania Macucule, Baltazar Marranangumbe, Angelica Suzana Baptista, Jorge Manguele, Joaquim Koivula, Tuija Streicher, Elizabeth Maria Warren, Robin Mark Kallenius, Gunilla van Helden, Paul Correia-Neves, M |
author_role |
author |
author2 |
Rito, Teresa Ghebremichael, Solomon Muhate, Nuelma Maxhuza, Gabriel Macuamule, Custodia Moiane, Ivania Macucule, Baltazar Marranangumbe, Angelica Suzana Baptista, Jorge Manguele, Joaquim Koivula, Tuija Streicher, Elizabeth Maria Warren, Robin Mark Kallenius, Gunilla van Helden, Paul Correia-Neves, M |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Machado, Adelina Rito, Teresa Ghebremichael, Solomon Muhate, Nuelma Maxhuza, Gabriel Macuamule, Custodia Moiane, Ivania Macucule, Baltazar Marranangumbe, Angelica Suzana Baptista, Jorge Manguele, Joaquim Koivula, Tuija Streicher, Elizabeth Maria Warren, Robin Mark Kallenius, Gunilla van Helden, Paul Correia-Neves, M |
dc.subject.por.fl_str_mv |
Animals Cattle Genotype Molecular Epidemiology Molecular Typing Mozambique Mycobacterium bovis Tuberculosis, Bovine Zoonoses Disease Transmission, Infectious Genetic Variation Ciências Médicas::Medicina Básica Science & Technology |
topic |
Animals Cattle Genotype Molecular Epidemiology Molecular Typing Mozambique Mycobacterium bovis Tuberculosis, Bovine Zoonoses Disease Transmission, Infectious Genetic Variation Ciências Médicas::Medicina Básica Science & Technology |
description |
Bovine tuberculosis is a zoonotic disease with largely unknown impact in Africa, with risk factors such as HIV and direct contact with animals or consumption of Mycobacterium bovis infected animal products. In order to understand and quantify this risk and design intervention strategies, good epidemiological studies are needed. Such studies can include molecular typing of M. bovis isolates. The aim of this study was to apply these tools to provide novel information concerning the distribution of bovine tuberculosis in cattle in Mozambique and thereby provide relevant information to guide policy development and strategies to contain the disease in livestock, and reduce the risk associated with transmission to humans. A collection of 178 M. bovis isolates was obtained from cattle in Mozambique. Using spoligotyping and regions of difference analysis, we classified the isolates into clonal complexes, thus reporting the first characterisation of M. bovis strains in this region. Data from MIRU-VNTR typing was used to compare isolates from a number of African countries, revealing a deeply geographically structured diversity of M. bovis. Eastern Africa appears to show high diversity, suggesting deep evolution in that region. The diversity of M. bovis in Africa does not seem to be a function of recent importation of animals, but is probably maintained within each particular region by constant reinfection from reservoir animals. Understanding the transmission routes of M. bovis in Mozambique and elsewhere is essential in order to focus public health and veterinary resources to contain bovine tuberculosis. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01 2018-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/57875 |
url |
http://hdl.handle.net/1822/57875 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Machado, A., Rito, T., Ghebremichael, S., Muhate, N., et. al.(2018). Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique. PLoS neglected tropical diseases, 12(1), e0006147 1935-2727 1935-2735 10.1371/journal.pntd.0006147 29346413 https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0006147 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science (PLOS) |
publisher.none.fl_str_mv |
Public Library of Science (PLOS) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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