The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.9/3967 |
Resumo: | ABSTRACT: Chrypthecodinium cohnii lipids have been almost exclusively used as a source of Docosahexaenoic acid (DHA). Such an approach wastes the remaining microalgal lipid fraction. The present work presents a novel process to produce C. cohnii biomass, using low-cost industrial by-products (raw glycerol and corn steep liquor), in a 7L-bioreactor, under fed-batch regime. At the end of the fermentation, the biomass concentration reached 9.2 g/L and the lipid content and lipid average productivity attained 28.0% (w/w dry cell weight) and 13.6 mg/L h, respectively. Afterwards the microalgal biomass underwent a saponification reaction to produce fatty acid (FA) soaps, which were further converted into FA ethyl ester (FA EE). C. cohnii FA EE mixture was then fractionated, using the urea complexation method at different temperatures, in order to obtain a polyunsaturated fatty acid ethyl ester (PUFA EE) rich fraction, that could be used for food/pharmaceutical/cosmetic purposes, and a saturated fatty acid ethyl ester (SAT EE) rich fraction, which could be used as biodiesel. The temperature that promoted the best separation between PUFA and SAT EE, was -18 degrees C, resulting in a liquid fraction with 91.6% (w/w) DHA, and a solid phase with 88.2% of SAT and monounsaturated fatty acid ethyl ester (MONOUNSAT), which could be used for biodiesel purposes after a hydrogenation step. |
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The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil FractionsBiorefineryBiodieselCrypthecodinium cohniiLipidsGlycerolDHAABSTRACT: Chrypthecodinium cohnii lipids have been almost exclusively used as a source of Docosahexaenoic acid (DHA). Such an approach wastes the remaining microalgal lipid fraction. The present work presents a novel process to produce C. cohnii biomass, using low-cost industrial by-products (raw glycerol and corn steep liquor), in a 7L-bioreactor, under fed-batch regime. At the end of the fermentation, the biomass concentration reached 9.2 g/L and the lipid content and lipid average productivity attained 28.0% (w/w dry cell weight) and 13.6 mg/L h, respectively. Afterwards the microalgal biomass underwent a saponification reaction to produce fatty acid (FA) soaps, which were further converted into FA ethyl ester (FA EE). C. cohnii FA EE mixture was then fractionated, using the urea complexation method at different temperatures, in order to obtain a polyunsaturated fatty acid ethyl ester (PUFA EE) rich fraction, that could be used for food/pharmaceutical/cosmetic purposes, and a saturated fatty acid ethyl ester (SAT EE) rich fraction, which could be used as biodiesel. The temperature that promoted the best separation between PUFA and SAT EE, was -18 degrees C, resulting in a liquid fraction with 91.6% (w/w) DHA, and a solid phase with 88.2% of SAT and monounsaturated fatty acid ethyl ester (MONOUNSAT), which could be used for biodiesel purposes after a hydrogenation step.MDPIRepositório do LNEGMoniz, PatriciaMartins, DanielaOliveira, Ana CristinaReis, AlbertoSilva, Teresa Lopes da2022-11-30T16:37:51Z2022-102022-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.9/3967engMoniz, Patrícia... [et.al.] - The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions. In: Fermentation, 2022, vol. 8(10), article nº 50210.3390/fermentation81005022311-5637info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-12-04T04:45:16Zoai:repositorio.lneg.pt:10400.9/3967Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:15:26.784020Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions |
title |
The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions |
spellingShingle |
The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions Moniz, Patricia Biorefinery Biodiesel Crypthecodinium cohnii Lipids Glycerol DHA |
title_short |
The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions |
title_full |
The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions |
title_fullStr |
The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions |
title_full_unstemmed |
The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions |
title_sort |
The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions |
author |
Moniz, Patricia |
author_facet |
Moniz, Patricia Martins, Daniela Oliveira, Ana Cristina Reis, Alberto Silva, Teresa Lopes da |
author_role |
author |
author2 |
Martins, Daniela Oliveira, Ana Cristina Reis, Alberto Silva, Teresa Lopes da |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório do LNEG |
dc.contributor.author.fl_str_mv |
Moniz, Patricia Martins, Daniela Oliveira, Ana Cristina Reis, Alberto Silva, Teresa Lopes da |
dc.subject.por.fl_str_mv |
Biorefinery Biodiesel Crypthecodinium cohnii Lipids Glycerol DHA |
topic |
Biorefinery Biodiesel Crypthecodinium cohnii Lipids Glycerol DHA |
description |
ABSTRACT: Chrypthecodinium cohnii lipids have been almost exclusively used as a source of Docosahexaenoic acid (DHA). Such an approach wastes the remaining microalgal lipid fraction. The present work presents a novel process to produce C. cohnii biomass, using low-cost industrial by-products (raw glycerol and corn steep liquor), in a 7L-bioreactor, under fed-batch regime. At the end of the fermentation, the biomass concentration reached 9.2 g/L and the lipid content and lipid average productivity attained 28.0% (w/w dry cell weight) and 13.6 mg/L h, respectively. Afterwards the microalgal biomass underwent a saponification reaction to produce fatty acid (FA) soaps, which were further converted into FA ethyl ester (FA EE). C. cohnii FA EE mixture was then fractionated, using the urea complexation method at different temperatures, in order to obtain a polyunsaturated fatty acid ethyl ester (PUFA EE) rich fraction, that could be used for food/pharmaceutical/cosmetic purposes, and a saturated fatty acid ethyl ester (SAT EE) rich fraction, which could be used as biodiesel. The temperature that promoted the best separation between PUFA and SAT EE, was -18 degrees C, resulting in a liquid fraction with 91.6% (w/w) DHA, and a solid phase with 88.2% of SAT and monounsaturated fatty acid ethyl ester (MONOUNSAT), which could be used for biodiesel purposes after a hydrogenation step. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-30T16:37:51Z 2022-10 2022-10-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.9/3967 |
url |
http://hdl.handle.net/10400.9/3967 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Moniz, Patrícia... [et.al.] - The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions. In: Fermentation, 2022, vol. 8(10), article nº 502 10.3390/fermentation8100502 2311-5637 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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