The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions

Detalhes bibliográficos
Autor(a) principal: Moniz, Patricia
Data de Publicação: 2022
Outros Autores: Martins, Daniela, Oliveira, Ana Cristina, Reis, Alberto, Silva, Teresa Lopes da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.9/3967
Resumo: ABSTRACT: Chrypthecodinium cohnii lipids have been almost exclusively used as a source of Docosahexaenoic acid (DHA). Such an approach wastes the remaining microalgal lipid fraction. The present work presents a novel process to produce C. cohnii biomass, using low-cost industrial by-products (raw glycerol and corn steep liquor), in a 7L-bioreactor, under fed-batch regime. At the end of the fermentation, the biomass concentration reached 9.2 g/L and the lipid content and lipid average productivity attained 28.0% (w/w dry cell weight) and 13.6 mg/L h, respectively. Afterwards the microalgal biomass underwent a saponification reaction to produce fatty acid (FA) soaps, which were further converted into FA ethyl ester (FA EE). C. cohnii FA EE mixture was then fractionated, using the urea complexation method at different temperatures, in order to obtain a polyunsaturated fatty acid ethyl ester (PUFA EE) rich fraction, that could be used for food/pharmaceutical/cosmetic purposes, and a saturated fatty acid ethyl ester (SAT EE) rich fraction, which could be used as biodiesel. The temperature that promoted the best separation between PUFA and SAT EE, was -18 degrees C, resulting in a liquid fraction with 91.6% (w/w) DHA, and a solid phase with 88.2% of SAT and monounsaturated fatty acid ethyl ester (MONOUNSAT), which could be used for biodiesel purposes after a hydrogenation step.
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spelling The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil FractionsBiorefineryBiodieselCrypthecodinium cohniiLipidsGlycerolDHAABSTRACT: Chrypthecodinium cohnii lipids have been almost exclusively used as a source of Docosahexaenoic acid (DHA). Such an approach wastes the remaining microalgal lipid fraction. The present work presents a novel process to produce C. cohnii biomass, using low-cost industrial by-products (raw glycerol and corn steep liquor), in a 7L-bioreactor, under fed-batch regime. At the end of the fermentation, the biomass concentration reached 9.2 g/L and the lipid content and lipid average productivity attained 28.0% (w/w dry cell weight) and 13.6 mg/L h, respectively. Afterwards the microalgal biomass underwent a saponification reaction to produce fatty acid (FA) soaps, which were further converted into FA ethyl ester (FA EE). C. cohnii FA EE mixture was then fractionated, using the urea complexation method at different temperatures, in order to obtain a polyunsaturated fatty acid ethyl ester (PUFA EE) rich fraction, that could be used for food/pharmaceutical/cosmetic purposes, and a saturated fatty acid ethyl ester (SAT EE) rich fraction, which could be used as biodiesel. The temperature that promoted the best separation between PUFA and SAT EE, was -18 degrees C, resulting in a liquid fraction with 91.6% (w/w) DHA, and a solid phase with 88.2% of SAT and monounsaturated fatty acid ethyl ester (MONOUNSAT), which could be used for biodiesel purposes after a hydrogenation step.MDPIRepositório do LNEGMoniz, PatriciaMartins, DanielaOliveira, Ana CristinaReis, AlbertoSilva, Teresa Lopes da2022-11-30T16:37:51Z2022-102022-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.9/3967engMoniz, Patrícia... [et.al.] - The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions. In: Fermentation, 2022, vol. 8(10), article nº 50210.3390/fermentation81005022311-5637info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-12-04T04:45:16Zoai:repositorio.lneg.pt:10400.9/3967Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:15:26.784020Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions
title The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions
spellingShingle The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions
Moniz, Patricia
Biorefinery
Biodiesel
Crypthecodinium cohnii
Lipids
Glycerol
DHA
title_short The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions
title_full The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions
title_fullStr The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions
title_full_unstemmed The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions
title_sort The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions
author Moniz, Patricia
author_facet Moniz, Patricia
Martins, Daniela
Oliveira, Ana Cristina
Reis, Alberto
Silva, Teresa Lopes da
author_role author
author2 Martins, Daniela
Oliveira, Ana Cristina
Reis, Alberto
Silva, Teresa Lopes da
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório do LNEG
dc.contributor.author.fl_str_mv Moniz, Patricia
Martins, Daniela
Oliveira, Ana Cristina
Reis, Alberto
Silva, Teresa Lopes da
dc.subject.por.fl_str_mv Biorefinery
Biodiesel
Crypthecodinium cohnii
Lipids
Glycerol
DHA
topic Biorefinery
Biodiesel
Crypthecodinium cohnii
Lipids
Glycerol
DHA
description ABSTRACT: Chrypthecodinium cohnii lipids have been almost exclusively used as a source of Docosahexaenoic acid (DHA). Such an approach wastes the remaining microalgal lipid fraction. The present work presents a novel process to produce C. cohnii biomass, using low-cost industrial by-products (raw glycerol and corn steep liquor), in a 7L-bioreactor, under fed-batch regime. At the end of the fermentation, the biomass concentration reached 9.2 g/L and the lipid content and lipid average productivity attained 28.0% (w/w dry cell weight) and 13.6 mg/L h, respectively. Afterwards the microalgal biomass underwent a saponification reaction to produce fatty acid (FA) soaps, which were further converted into FA ethyl ester (FA EE). C. cohnii FA EE mixture was then fractionated, using the urea complexation method at different temperatures, in order to obtain a polyunsaturated fatty acid ethyl ester (PUFA EE) rich fraction, that could be used for food/pharmaceutical/cosmetic purposes, and a saturated fatty acid ethyl ester (SAT EE) rich fraction, which could be used as biodiesel. The temperature that promoted the best separation between PUFA and SAT EE, was -18 degrees C, resulting in a liquid fraction with 91.6% (w/w) DHA, and a solid phase with 88.2% of SAT and monounsaturated fatty acid ethyl ester (MONOUNSAT), which could be used for biodiesel purposes after a hydrogenation step.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-30T16:37:51Z
2022-10
2022-10-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.9/3967
url http://hdl.handle.net/10400.9/3967
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Moniz, Patrícia... [et.al.] - The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions. In: Fermentation, 2022, vol. 8(10), article nº 502
10.3390/fermentation8100502
2311-5637
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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