Assessment of the presence of Acinetobacter spp. resistant to β-lactams in commercial ready-to-eat salad samples
Autor(a) principal: | |
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Data de Publicação: | 2024 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/25033 |
Resumo: | Acinetobacter baumannii is a well-known nosocomial infection causing agent. However, other Acinetobacter spp. have also been implicated in cases of human infection. Additionally, these bacteria are known for the development of antibiotic resistance thus making the treatment of the infections they cause, challenging. Due to their relevance in clinical setups less attention has been paid to their presence in foods, and its relation with infection/ dissemination routes. In the current study commercial Ready-To-Eat (RTE) salads were analyzed seeking for antibiotic resistant Acinetobacter spp. A preliminary screening allowed us to recover Gram-negative bacteria resistant to β – lactams using cefotaxime, third generation cephalosporins, as the selective agent, and this was followed by identification with CHROMagar™ Acinetobacter and 16S rDNA sequencing. Finally, the isolates identified as Acinetobacter spp. were reanalyzed by PCR to determine the presence of nine potential Extended Spectrum β Lactamases (ESBL). Two commercial RTE salad brands were included in the study (2 batches per brand and 8 samples of each batch making a total of 32 independent samples), and compared against an organic lettuce. High concentrations of β – lactam, resistant bacteria were found in all the samples tested (5 log CFU/g). Additionally, 209 isolates were phenotypically characterized on CHROMagar Acinetobacter. Finally, PCR analysis identified the presence of different ESBL genes, being positive for blaACC, blaSHV, blaDHA and blaVEB; out of these, blaACC was the most prevalent. None of the isolates screened were positive for more than one gene. To conclude, it is important to highlight the fact that pathogenic species within the genus Acinetobacter spp., other than A. baumannii, have been identified bearing resistance genes not typically associated to these microorganisms highlight the importance of continuous surveillance. |
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Assessment of the presence of Acinetobacter spp. resistant to β-lactams in commercial ready-to-eat salad samplesAcinetobacter spp.Antibiotic resistanceβ-lactamGram-negativeReady-to-eatSaladsAcinetobacter baumannii is a well-known nosocomial infection causing agent. However, other Acinetobacter spp. have also been implicated in cases of human infection. Additionally, these bacteria are known for the development of antibiotic resistance thus making the treatment of the infections they cause, challenging. Due to their relevance in clinical setups less attention has been paid to their presence in foods, and its relation with infection/ dissemination routes. In the current study commercial Ready-To-Eat (RTE) salads were analyzed seeking for antibiotic resistant Acinetobacter spp. A preliminary screening allowed us to recover Gram-negative bacteria resistant to β – lactams using cefotaxime, third generation cephalosporins, as the selective agent, and this was followed by identification with CHROMagar™ Acinetobacter and 16S rDNA sequencing. Finally, the isolates identified as Acinetobacter spp. were reanalyzed by PCR to determine the presence of nine potential Extended Spectrum β Lactamases (ESBL). Two commercial RTE salad brands were included in the study (2 batches per brand and 8 samples of each batch making a total of 32 independent samples), and compared against an organic lettuce. High concentrations of β – lactam, resistant bacteria were found in all the samples tested (5 log CFU/g). Additionally, 209 isolates were phenotypically characterized on CHROMagar Acinetobacter. Finally, PCR analysis identified the presence of different ESBL genes, being positive for blaACC, blaSHV, blaDHA and blaVEB; out of these, blaACC was the most prevalent. None of the isolates screened were positive for more than one gene. To conclude, it is important to highlight the fact that pathogenic species within the genus Acinetobacter spp., other than A. baumannii, have been identified bearing resistance genes not typically associated to these microorganisms highlight the importance of continuous surveillance.ElsevierRepositório Científico do Instituto Politécnico do PortoCosta-Ribeiro, AnaAzinheiro, SarahMota, SandraPrado, MartaLamas, AlexandreGarrido-Maestu, Alejandro2024-02-20T09:09:32Z2024-042024-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/25033engCosta-Ribeiro, A., Azinheiro, S., Mota, S., Prado, M., Lamas, A., & Garrido-Maestu, A. (2024). Assessment of the presence of Acinetobacter spp. Resistant to β-lactams in commercial ready-to-eat salad samples. Food Microbiology, 118, 104410. https://doi.org/10.1016/j.fm.2023.1044100740-002010.1016/j.fm.2023.104410info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-21T01:46:52Zoai:recipp.ipp.pt:10400.22/25033Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:39:20.354268Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Assessment of the presence of Acinetobacter spp. resistant to β-lactams in commercial ready-to-eat salad samples |
title |
Assessment of the presence of Acinetobacter spp. resistant to β-lactams in commercial ready-to-eat salad samples |
spellingShingle |
Assessment of the presence of Acinetobacter spp. resistant to β-lactams in commercial ready-to-eat salad samples Costa-Ribeiro, Ana Acinetobacter spp. Antibiotic resistance β-lactam Gram-negative Ready-to-eat Salads |
title_short |
Assessment of the presence of Acinetobacter spp. resistant to β-lactams in commercial ready-to-eat salad samples |
title_full |
Assessment of the presence of Acinetobacter spp. resistant to β-lactams in commercial ready-to-eat salad samples |
title_fullStr |
Assessment of the presence of Acinetobacter spp. resistant to β-lactams in commercial ready-to-eat salad samples |
title_full_unstemmed |
Assessment of the presence of Acinetobacter spp. resistant to β-lactams in commercial ready-to-eat salad samples |
title_sort |
Assessment of the presence of Acinetobacter spp. resistant to β-lactams in commercial ready-to-eat salad samples |
author |
Costa-Ribeiro, Ana |
author_facet |
Costa-Ribeiro, Ana Azinheiro, Sarah Mota, Sandra Prado, Marta Lamas, Alexandre Garrido-Maestu, Alejandro |
author_role |
author |
author2 |
Azinheiro, Sarah Mota, Sandra Prado, Marta Lamas, Alexandre Garrido-Maestu, Alejandro |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Costa-Ribeiro, Ana Azinheiro, Sarah Mota, Sandra Prado, Marta Lamas, Alexandre Garrido-Maestu, Alejandro |
dc.subject.por.fl_str_mv |
Acinetobacter spp. Antibiotic resistance β-lactam Gram-negative Ready-to-eat Salads |
topic |
Acinetobacter spp. Antibiotic resistance β-lactam Gram-negative Ready-to-eat Salads |
description |
Acinetobacter baumannii is a well-known nosocomial infection causing agent. However, other Acinetobacter spp. have also been implicated in cases of human infection. Additionally, these bacteria are known for the development of antibiotic resistance thus making the treatment of the infections they cause, challenging. Due to their relevance in clinical setups less attention has been paid to their presence in foods, and its relation with infection/ dissemination routes. In the current study commercial Ready-To-Eat (RTE) salads were analyzed seeking for antibiotic resistant Acinetobacter spp. A preliminary screening allowed us to recover Gram-negative bacteria resistant to β – lactams using cefotaxime, third generation cephalosporins, as the selective agent, and this was followed by identification with CHROMagar™ Acinetobacter and 16S rDNA sequencing. Finally, the isolates identified as Acinetobacter spp. were reanalyzed by PCR to determine the presence of nine potential Extended Spectrum β Lactamases (ESBL). Two commercial RTE salad brands were included in the study (2 batches per brand and 8 samples of each batch making a total of 32 independent samples), and compared against an organic lettuce. High concentrations of β – lactam, resistant bacteria were found in all the samples tested (5 log CFU/g). Additionally, 209 isolates were phenotypically characterized on CHROMagar Acinetobacter. Finally, PCR analysis identified the presence of different ESBL genes, being positive for blaACC, blaSHV, blaDHA and blaVEB; out of these, blaACC was the most prevalent. None of the isolates screened were positive for more than one gene. To conclude, it is important to highlight the fact that pathogenic species within the genus Acinetobacter spp., other than A. baumannii, have been identified bearing resistance genes not typically associated to these microorganisms highlight the importance of continuous surveillance. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-02-20T09:09:32Z 2024-04 2024-04-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/25033 |
url |
http://hdl.handle.net/10400.22/25033 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Costa-Ribeiro, A., Azinheiro, S., Mota, S., Prado, M., Lamas, A., & Garrido-Maestu, A. (2024). Assessment of the presence of Acinetobacter spp. Resistant to β-lactams in commercial ready-to-eat salad samples. Food Microbiology, 118, 104410. https://doi.org/10.1016/j.fm.2023.104410 0740-0020 10.1016/j.fm.2023.104410 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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