In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foods

Detalhes bibliográficos
Autor(a) principal: Amador, Paula
Data de Publicação: 2011
Outros Autores: Fernandes, Rúben, Duarte, I., Brito, Luísa, Prudêncio, Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/3601
Resumo: The principal aim of this study was to investigate the possibility of transference to Escherichia coli of β-lactam resistance genes found in bacteria isolated from ready-to-eat (RTE) Portuguese traditional food. From previous screenings, 128 β-lactam resistant isolates (from different types of cheese and of delicatessen meats), largely from the Enterobacteriaceae family were selected and 31.3% of them proved to transfer resistance determinants in transconjugation assays. Multiplex PCR in donor and transconjugant isolates did not detect bla CTX, bla SHV and bla OXY, but bla TEM was present in 85% of them, while two new TEMs (TEM-179 and TEM-180) were identified in two isolates. The sequencing of these amplicons showed identity between donor and transconjugant genes indicating in vitro plasmid DNA transfer. These results suggest that if there is an exchange of genes in natural conditions, the consumption of RTE foods, particularly with high levels of Enterobacteriaceae, can contribute to the spread of antibiotic resistance.
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spelling In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foodsReady-to-eat (RTE) foodβ-lactam-resistant Enterobacteriaceaebla TEM-1 geneTransconjugantsThe principal aim of this study was to investigate the possibility of transference to Escherichia coli of β-lactam resistance genes found in bacteria isolated from ready-to-eat (RTE) Portuguese traditional food. From previous screenings, 128 β-lactam resistant isolates (from different types of cheese and of delicatessen meats), largely from the Enterobacteriaceae family were selected and 31.3% of them proved to transfer resistance determinants in transconjugation assays. Multiplex PCR in donor and transconjugant isolates did not detect bla CTX, bla SHV and bla OXY, but bla TEM was present in 85% of them, while two new TEMs (TEM-179 and TEM-180) were identified in two isolates. The sequencing of these amplicons showed identity between donor and transconjugant genes indicating in vitro plasmid DNA transfer. These results suggest that if there is an exchange of genes in natural conditions, the consumption of RTE foods, particularly with high levels of Enterobacteriaceae, can contribute to the spread of antibiotic resistance.SpringerRepositório Científico do Instituto Politécnico do PortoAmador, PaulaFernandes, RúbenDuarte, I.Brito, LuísaPrudêncio, Cristina2014-02-03T11:36:09Z20112013-07-25T21:03:01Z2011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdftext/plain; charset=utf-8http://hdl.handle.net/10400.22/3601eng0959-399310.1007/s11274-010-0635-9info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:41:17Zoai:recipp.ipp.pt:10400.22/3601Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:22:56.770812Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foods
title In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foods
spellingShingle In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foods
Amador, Paula
Ready-to-eat (RTE) food
β-lactam-resistant Enterobacteriaceae
bla TEM-1 gene
Transconjugants
title_short In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foods
title_full In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foods
title_fullStr In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foods
title_full_unstemmed In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foods
title_sort In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foods
author Amador, Paula
author_facet Amador, Paula
Fernandes, Rúben
Duarte, I.
Brito, Luísa
Prudêncio, Cristina
author_role author
author2 Fernandes, Rúben
Duarte, I.
Brito, Luísa
Prudêncio, Cristina
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Amador, Paula
Fernandes, Rúben
Duarte, I.
Brito, Luísa
Prudêncio, Cristina
dc.subject.por.fl_str_mv Ready-to-eat (RTE) food
β-lactam-resistant Enterobacteriaceae
bla TEM-1 gene
Transconjugants
topic Ready-to-eat (RTE) food
β-lactam-resistant Enterobacteriaceae
bla TEM-1 gene
Transconjugants
description The principal aim of this study was to investigate the possibility of transference to Escherichia coli of β-lactam resistance genes found in bacteria isolated from ready-to-eat (RTE) Portuguese traditional food. From previous screenings, 128 β-lactam resistant isolates (from different types of cheese and of delicatessen meats), largely from the Enterobacteriaceae family were selected and 31.3% of them proved to transfer resistance determinants in transconjugation assays. Multiplex PCR in donor and transconjugant isolates did not detect bla CTX, bla SHV and bla OXY, but bla TEM was present in 85% of them, while two new TEMs (TEM-179 and TEM-180) were identified in two isolates. The sequencing of these amplicons showed identity between donor and transconjugant genes indicating in vitro plasmid DNA transfer. These results suggest that if there is an exchange of genes in natural conditions, the consumption of RTE foods, particularly with high levels of Enterobacteriaceae, can contribute to the spread of antibiotic resistance.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
2013-07-25T21:03:01Z
2014-02-03T11:36:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/3601
url http://hdl.handle.net/10400.22/3601
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0959-3993
10.1007/s11274-010-0635-9
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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