Development of colorimetric cellulose-based test-strip for the rapid detection of antibodies against SARS-CoV2 virus
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/21874 |
Resumo: | Given the pandemic situation, there is an urgent need for an accurate test to monitor antibodies anti-SARS-CoV-2, providing crucial epidemiological and clinical information to monitor the evolution of coronavirus disease in 2019 (COVID-19) and to stratify the immunized and asymptomatic population. Therefore, this paper describes a new cellulosebased test strip for rapid and cost-efective quantitative detection of antibodies to SARS-CoV2 virus by colorimetric transduction. For this purpose, Whatman paper was chemically modifed with sodium metaperiodate to introduce aldehyde groups on its surface. Subsequently, the spike protein of the virus is covalently bound by forming an imine group. The chemical control of cellulose paper modifcation was evaluated by Fourier transform infrared spectroscopy, thermogravimetry and contact angle analysis. Colorimetric detection of the antibodies was performed by a conventional staining method using Ponceau S solution as the dye. Color analysis was performed after image acquisition with a smartphone using Image J software. The color intensity varied linearly with the logarithm of the anti-S concentration (from 10 ng/mL to 1 μg/mL) in 500-fold diluted serum samples when plotted against the green coordinate extracted from digital images. The test strip was selective in the presence of nucleocapsid antibodies, urea, glucose, and bovine serum albumin with less than 15% interference, and detection of antibodies in human serum was successfully performed. Overall, this is a simple and afordable design that can be readily used for mass Abstract Given the pandemic situation, there is an population screening and does not require sophisticated equipment or qualifed personnel |
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Development of colorimetric cellulose-based test-strip for the rapid detection of antibodies against SARS-CoV2 virusColorimetricDyeProtein spikeSerumTest-stripsGiven the pandemic situation, there is an urgent need for an accurate test to monitor antibodies anti-SARS-CoV-2, providing crucial epidemiological and clinical information to monitor the evolution of coronavirus disease in 2019 (COVID-19) and to stratify the immunized and asymptomatic population. Therefore, this paper describes a new cellulosebased test strip for rapid and cost-efective quantitative detection of antibodies to SARS-CoV2 virus by colorimetric transduction. For this purpose, Whatman paper was chemically modifed with sodium metaperiodate to introduce aldehyde groups on its surface. Subsequently, the spike protein of the virus is covalently bound by forming an imine group. The chemical control of cellulose paper modifcation was evaluated by Fourier transform infrared spectroscopy, thermogravimetry and contact angle analysis. Colorimetric detection of the antibodies was performed by a conventional staining method using Ponceau S solution as the dye. Color analysis was performed after image acquisition with a smartphone using Image J software. The color intensity varied linearly with the logarithm of the anti-S concentration (from 10 ng/mL to 1 μg/mL) in 500-fold diluted serum samples when plotted against the green coordinate extracted from digital images. The test strip was selective in the presence of nucleocapsid antibodies, urea, glucose, and bovine serum albumin with less than 15% interference, and detection of antibodies in human serum was successfully performed. Overall, this is a simple and afordable design that can be readily used for mass Abstract Given the pandemic situation, there is an population screening and does not require sophisticated equipment or qualifed personnelThis project was a fnancially supported through the project, TecniCov (POCI-01-02B7-FEDER-069745), cofunded by FEDER through COMPETE2020 and Lisboa2020.SpringerRepositório Científico do Instituto Politécnico do PortoCorreia, Bárbara P.Sousa, Mariana P.Sousa, Cristina E. A.Mateus, DanielaSebastião, Ana IsabelCruz, Maria TeresaMatos, Ana MiguelSantos Pereira, Ana CláudiaMoreira, Felismina20222035-01-01T00:00:00Z2022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/21874eng10.1007/s10570-022-04808-ymetadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:18:12Zoai:recipp.ipp.pt:10400.22/21874Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:41:57.367464Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development of colorimetric cellulose-based test-strip for the rapid detection of antibodies against SARS-CoV2 virus |
title |
Development of colorimetric cellulose-based test-strip for the rapid detection of antibodies against SARS-CoV2 virus |
spellingShingle |
Development of colorimetric cellulose-based test-strip for the rapid detection of antibodies against SARS-CoV2 virus Correia, Bárbara P. Colorimetric Dye Protein spike Serum Test-strips |
title_short |
Development of colorimetric cellulose-based test-strip for the rapid detection of antibodies against SARS-CoV2 virus |
title_full |
Development of colorimetric cellulose-based test-strip for the rapid detection of antibodies against SARS-CoV2 virus |
title_fullStr |
Development of colorimetric cellulose-based test-strip for the rapid detection of antibodies against SARS-CoV2 virus |
title_full_unstemmed |
Development of colorimetric cellulose-based test-strip for the rapid detection of antibodies against SARS-CoV2 virus |
title_sort |
Development of colorimetric cellulose-based test-strip for the rapid detection of antibodies against SARS-CoV2 virus |
author |
Correia, Bárbara P. |
author_facet |
Correia, Bárbara P. Sousa, Mariana P. Sousa, Cristina E. A. Mateus, Daniela Sebastião, Ana Isabel Cruz, Maria Teresa Matos, Ana Miguel Santos Pereira, Ana Cláudia Moreira, Felismina |
author_role |
author |
author2 |
Sousa, Mariana P. Sousa, Cristina E. A. Mateus, Daniela Sebastião, Ana Isabel Cruz, Maria Teresa Matos, Ana Miguel Santos Pereira, Ana Cláudia Moreira, Felismina |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Correia, Bárbara P. Sousa, Mariana P. Sousa, Cristina E. A. Mateus, Daniela Sebastião, Ana Isabel Cruz, Maria Teresa Matos, Ana Miguel Santos Pereira, Ana Cláudia Moreira, Felismina |
dc.subject.por.fl_str_mv |
Colorimetric Dye Protein spike Serum Test-strips |
topic |
Colorimetric Dye Protein spike Serum Test-strips |
description |
Given the pandemic situation, there is an urgent need for an accurate test to monitor antibodies anti-SARS-CoV-2, providing crucial epidemiological and clinical information to monitor the evolution of coronavirus disease in 2019 (COVID-19) and to stratify the immunized and asymptomatic population. Therefore, this paper describes a new cellulosebased test strip for rapid and cost-efective quantitative detection of antibodies to SARS-CoV2 virus by colorimetric transduction. For this purpose, Whatman paper was chemically modifed with sodium metaperiodate to introduce aldehyde groups on its surface. Subsequently, the spike protein of the virus is covalently bound by forming an imine group. The chemical control of cellulose paper modifcation was evaluated by Fourier transform infrared spectroscopy, thermogravimetry and contact angle analysis. Colorimetric detection of the antibodies was performed by a conventional staining method using Ponceau S solution as the dye. Color analysis was performed after image acquisition with a smartphone using Image J software. The color intensity varied linearly with the logarithm of the anti-S concentration (from 10 ng/mL to 1 μg/mL) in 500-fold diluted serum samples when plotted against the green coordinate extracted from digital images. The test strip was selective in the presence of nucleocapsid antibodies, urea, glucose, and bovine serum albumin with less than 15% interference, and detection of antibodies in human serum was successfully performed. Overall, this is a simple and afordable design that can be readily used for mass Abstract Given the pandemic situation, there is an population screening and does not require sophisticated equipment or qualifed personnel |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 2022-01-01T00:00:00Z 2035-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/21874 |
url |
http://hdl.handle.net/10400.22/21874 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1007/s10570-022-04808-y |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131506189271040 |