A simple aptamer-based colorimetric assay for rapid detection of C-reactive protein using gold nanoparticles
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/36701 |
Resumo: | C-reactive protein (CRP) is a clinical biomarker for inflammatory diseases. In this work, we present a simple and fast colorimetric method for CRP detection that employs citrate-capped gold nanoparticles (AuNPs) and a CRP-binding aptamer as sensing elements. The aptamer consisted in a guanine rich single-stranded DNA (ssDNA) that adsorbs onto the surface of the AuNPs. In the presence of the CRP, the ssDNA releases from the AuNPs surface to interact preferentially with the protein to form guanine-quadruplexes. The exposure of the unprotected AuNPs to buffer salts leads to aggregation and subsequent color change from red-wine to blue-purple that was readily seen by the naked eye. The AuNPs aggregation was monitored using UV-Vis spectroscopy and the CRP concentration in the samples could be correlated with the aggregation ratio (A670nm/A520nm). A linear sensing range of 0.889-20.7 μg/mL was found. The detection limit (LOD) was 1.2 μg/mL which is comparable to the typical clinical cutoff concentration in high-sensitivity CRP assays (1 μg/mL) and lower than the detection limit of nephelometric methods used in clinical practice. This method can provide a fast (5 min analysis time), simple, and sensitive way for CRP detection, with negligible interference of bovine serum albumin (BSA) up to concentrations of 100 nM. |
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7160 |
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A simple aptamer-based colorimetric assay for rapid detection of C-reactive protein using gold nanoparticlesC-reactive proteinGold nanoparticlesAptamerColorimetric detectionUV–Vis spectroscopyC-reactive protein (CRP) is a clinical biomarker for inflammatory diseases. In this work, we present a simple and fast colorimetric method for CRP detection that employs citrate-capped gold nanoparticles (AuNPs) and a CRP-binding aptamer as sensing elements. The aptamer consisted in a guanine rich single-stranded DNA (ssDNA) that adsorbs onto the surface of the AuNPs. In the presence of the CRP, the ssDNA releases from the AuNPs surface to interact preferentially with the protein to form guanine-quadruplexes. The exposure of the unprotected AuNPs to buffer salts leads to aggregation and subsequent color change from red-wine to blue-purple that was readily seen by the naked eye. The AuNPs aggregation was monitored using UV-Vis spectroscopy and the CRP concentration in the samples could be correlated with the aggregation ratio (A670nm/A520nm). A linear sensing range of 0.889-20.7 μg/mL was found. The detection limit (LOD) was 1.2 μg/mL which is comparable to the typical clinical cutoff concentration in high-sensitivity CRP assays (1 μg/mL) and lower than the detection limit of nephelometric methods used in clinical practice. This method can provide a fast (5 min analysis time), simple, and sensitive way for CRP detection, with negligible interference of bovine serum albumin (BSA) up to concentrations of 100 nM.Elsevier2023-03-28T15:22:33Z2020-07-01T00:00:00Z2020-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/36701eng0039-914010.1016/j.talanta.2020.120868António, MariaFerreira, RitaVitorino, RuiDaniel-da-Silva, Ana L.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:10:47Zoai:ria.ua.pt:10773/36701Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:07:25.920192Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A simple aptamer-based colorimetric assay for rapid detection of C-reactive protein using gold nanoparticles |
title |
A simple aptamer-based colorimetric assay for rapid detection of C-reactive protein using gold nanoparticles |
spellingShingle |
A simple aptamer-based colorimetric assay for rapid detection of C-reactive protein using gold nanoparticles António, Maria C-reactive protein Gold nanoparticles Aptamer Colorimetric detection UV–Vis spectroscopy |
title_short |
A simple aptamer-based colorimetric assay for rapid detection of C-reactive protein using gold nanoparticles |
title_full |
A simple aptamer-based colorimetric assay for rapid detection of C-reactive protein using gold nanoparticles |
title_fullStr |
A simple aptamer-based colorimetric assay for rapid detection of C-reactive protein using gold nanoparticles |
title_full_unstemmed |
A simple aptamer-based colorimetric assay for rapid detection of C-reactive protein using gold nanoparticles |
title_sort |
A simple aptamer-based colorimetric assay for rapid detection of C-reactive protein using gold nanoparticles |
author |
António, Maria |
author_facet |
António, Maria Ferreira, Rita Vitorino, Rui Daniel-da-Silva, Ana L. |
author_role |
author |
author2 |
Ferreira, Rita Vitorino, Rui Daniel-da-Silva, Ana L. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
António, Maria Ferreira, Rita Vitorino, Rui Daniel-da-Silva, Ana L. |
dc.subject.por.fl_str_mv |
C-reactive protein Gold nanoparticles Aptamer Colorimetric detection UV–Vis spectroscopy |
topic |
C-reactive protein Gold nanoparticles Aptamer Colorimetric detection UV–Vis spectroscopy |
description |
C-reactive protein (CRP) is a clinical biomarker for inflammatory diseases. In this work, we present a simple and fast colorimetric method for CRP detection that employs citrate-capped gold nanoparticles (AuNPs) and a CRP-binding aptamer as sensing elements. The aptamer consisted in a guanine rich single-stranded DNA (ssDNA) that adsorbs onto the surface of the AuNPs. In the presence of the CRP, the ssDNA releases from the AuNPs surface to interact preferentially with the protein to form guanine-quadruplexes. The exposure of the unprotected AuNPs to buffer salts leads to aggregation and subsequent color change from red-wine to blue-purple that was readily seen by the naked eye. The AuNPs aggregation was monitored using UV-Vis spectroscopy and the CRP concentration in the samples could be correlated with the aggregation ratio (A670nm/A520nm). A linear sensing range of 0.889-20.7 μg/mL was found. The detection limit (LOD) was 1.2 μg/mL which is comparable to the typical clinical cutoff concentration in high-sensitivity CRP assays (1 μg/mL) and lower than the detection limit of nephelometric methods used in clinical practice. This method can provide a fast (5 min analysis time), simple, and sensitive way for CRP detection, with negligible interference of bovine serum albumin (BSA) up to concentrations of 100 nM. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-07-01T00:00:00Z 2020-07-01 2023-03-28T15:22:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/36701 |
url |
http://hdl.handle.net/10773/36701 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0039-9140 10.1016/j.talanta.2020.120868 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137729350467584 |