Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch production by Aspergillus niger van Tieghem in stirred tank and internal-loop airlift bioreactors

Detalhes bibliográficos
Autor(a) principal: Michelin, Michele
Data de Publicação: 2013
Outros Autores: Mota, A., Polizeli, Maria de Lourdes T. M., Silva, Daniel Pereira da, Vicente, A. A., Teixeira, J. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/27520
Resumo: Oxygenation is an important parameter involved in the design and operation of mixing-sparging bioreactors and it can be analyzed by means of the oxygen mass transfer coefficient (kLa). In this study, batch fermentations in stirred tank bioreactor (STB) and airlift bioreactor (ALB) were operated under a range of kLa values, in attempts to optimize and compare the activities of extracellular xylanase and β-xylosidase synthesized by the fungus Aspergillus niger van Tieghem, using corncob as inducer source. The higher xylanase levels were observed on STB (9000 U l−1; kLa = 30 h−1), while the β-xylosidase production was similar in both bioreactors. However, when the enzymatic activity was compared for the same kLa value (12 h−1) in STB and ALB, the xylanase and β-xylosidase productions were higher in ALB (7000 U l−1 xylanase; 2100 U l−1 β-xylosidase) than STB (4500 U l−1 xylanase; 1800 U l−1 β-xylosidase).
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spelling Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch production by Aspergillus niger van Tieghem in stirred tank and internal-loop airlift bioreactorsBioreactorsXylanaseß-xylosidaseCorncobAspergillusk(L)aK a Lβ-XylosidaseScience & TechnologyOxygenation is an important parameter involved in the design and operation of mixing-sparging bioreactors and it can be analyzed by means of the oxygen mass transfer coefficient (kLa). In this study, batch fermentations in stirred tank bioreactor (STB) and airlift bioreactor (ALB) were operated under a range of kLa values, in attempts to optimize and compare the activities of extracellular xylanase and β-xylosidase synthesized by the fungus Aspergillus niger van Tieghem, using corncob as inducer source. The higher xylanase levels were observed on STB (9000 U l−1; kLa = 30 h−1), while the β-xylosidase production was similar in both bioreactors. However, when the enzymatic activity was compared for the same kLa value (12 h−1) in STB and ALB, the xylanase and β-xylosidase productions were higher in ALB (7000 U l−1 xylanase; 2100 U l−1 β-xylosidase) than STB (4500 U l−1 xylanase; 1800 U l−1 β-xylosidase).This work was supported by Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (1162/10-1 CAPES/Brazil) and Fundacao para a Ciencia e a Tecnologia (SFRH/BD/37082/2007 FCT/Portugal).ElsevierElsevier BVUniversidade do MinhoMichelin, MicheleMota, A.Polizeli, Maria de Lourdes T. M.Silva, Daniel Pereira daVicente, A. A.Teixeira, J. A.2013-05-052013-05-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/27520eng1369-703X1369-703X10.1016/j.bej.2013.09.002info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:22:17ZPortal AgregadorONG
dc.title.none.fl_str_mv Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch production by Aspergillus niger van Tieghem in stirred tank and internal-loop airlift bioreactors
title Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch production by Aspergillus niger van Tieghem in stirred tank and internal-loop airlift bioreactors
spellingShingle Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch production by Aspergillus niger van Tieghem in stirred tank and internal-loop airlift bioreactors
Michelin, Michele
Bioreactors
Xylanase
ß-xylosidase
Corncob
Aspergillus
k(L)a
K a L
β-Xylosidase
Science & Technology
title_short Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch production by Aspergillus niger van Tieghem in stirred tank and internal-loop airlift bioreactors
title_full Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch production by Aspergillus niger van Tieghem in stirred tank and internal-loop airlift bioreactors
title_fullStr Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch production by Aspergillus niger van Tieghem in stirred tank and internal-loop airlift bioreactors
title_full_unstemmed Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch production by Aspergillus niger van Tieghem in stirred tank and internal-loop airlift bioreactors
title_sort Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch production by Aspergillus niger van Tieghem in stirred tank and internal-loop airlift bioreactors
author Michelin, Michele
author_facet Michelin, Michele
Mota, A.
Polizeli, Maria de Lourdes T. M.
Silva, Daniel Pereira da
Vicente, A. A.
Teixeira, J. A.
author_role author
author2 Mota, A.
Polizeli, Maria de Lourdes T. M.
Silva, Daniel Pereira da
Vicente, A. A.
Teixeira, J. A.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Michelin, Michele
Mota, A.
Polizeli, Maria de Lourdes T. M.
Silva, Daniel Pereira da
Vicente, A. A.
Teixeira, J. A.
dc.subject.por.fl_str_mv Bioreactors
Xylanase
ß-xylosidase
Corncob
Aspergillus
k(L)a
K a L
β-Xylosidase
Science & Technology
topic Bioreactors
Xylanase
ß-xylosidase
Corncob
Aspergillus
k(L)a
K a L
β-Xylosidase
Science & Technology
description Oxygenation is an important parameter involved in the design and operation of mixing-sparging bioreactors and it can be analyzed by means of the oxygen mass transfer coefficient (kLa). In this study, batch fermentations in stirred tank bioreactor (STB) and airlift bioreactor (ALB) were operated under a range of kLa values, in attempts to optimize and compare the activities of extracellular xylanase and β-xylosidase synthesized by the fungus Aspergillus niger van Tieghem, using corncob as inducer source. The higher xylanase levels were observed on STB (9000 U l−1; kLa = 30 h−1), while the β-xylosidase production was similar in both bioreactors. However, when the enzymatic activity was compared for the same kLa value (12 h−1) in STB and ALB, the xylanase and β-xylosidase productions were higher in ALB (7000 U l−1 xylanase; 2100 U l−1 β-xylosidase) than STB (4500 U l−1 xylanase; 1800 U l−1 β-xylosidase).
publishDate 2013
dc.date.none.fl_str_mv 2013-05-05
2013-05-05T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/27520
url http://hdl.handle.net/1822/27520
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1369-703X
1369-703X
10.1016/j.bej.2013.09.002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
Elsevier BV
publisher.none.fl_str_mv Elsevier
Elsevier BV
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv
repository.mail.fl_str_mv
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