Fed-batch fermentation of olive mill wastewaters for lipase production

• Autor(a) principal: Gonçalves, Cristiana
• Data de Publicação: 2012
• Outros Autores: Oliveira, Felisbela Maria Araújo, Pereira, C., Belo, Isabel
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/23380
• topic: Candida species; Yarrowia lipolytica; Olive mill wastewater; Fed-batch fermentation; lipase; Science & Technology

In the Mediterranean basin countries, huge amounts of olive mill wastewaters (OMW) are produced by the olive oil industry. It constitutes a serious environmental problem, nevertheless its composition turns OMW into a potential growth medium to lipolytic microorganisms. The aim of this work was to study lipase production as well as OMW degradation in fed-batch cultures of Candida cylindracea CBS 7869, Candida rugosa CBS 2275 and Yarrowia lipolytica W29 (ATCC 20460). Besides the improvement of lipase production, the fed-batch approach enhanced the effluent degradation, since it led to good COD and lipids reduction, both higher than 50%. C. rugosa achieved the highest value of lipase productivity (130 U L−1 h−1), in parallel with highest lipids reduction (77%). This study demonstrates thatOMWare becoming a competitive and valuable growth medium in fermentation processes with lipolytic microorganisms. The fed-batch strategy used proved to be an efficient approach to enhance lipase production from OMW and to reduce significantly the final organic load of the medium.