Olive and sunflower cakes as suitable substrates for lipase production by Yarrowia spp.: From flasks to bioreactor
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/86024 |
Resumo: | Solid state fermentation is a biotechnological process that allows the utilization of agro-industrial by-products as solid substrates for the growth of microorganisms, including filamentous fungi and yeasts, with the simultaneous production of added value compounds, like enzymes. Oil cakes, such as olive and sunflower cakes, are generated during vegetable oil extraction and have received great interest as solid substrates in SSF processes. In the present work, a 50% (w/w) mixture of olive and sunflower cakes was used as substrate in SSF for lipase production by several strains of Y. lipolytica and Y. divulgata. The strain of Y. lipolytica W29 was the best lipase producer under small scale SSF and studies were conducted to address important factors in scaling-up the process. Different bioreactors were used and the effect of aeration and agitation on lipase production was studied. Higher lipase activity was obtained in tray bioreactors, reaching 85 U/g (dry mass of substrates) after 48h of SSF, while the operation in a horizontal drum bioreactor favored yeast growth, resulting in the highest yeast biomass production and a delay in lipase maximum activity. The scale-up of SSF for lipase production by Y. lipolytica has been successfully performed, showing that SSF is an interesting and eco-friendly bioprocessing strategy for the valorization of by-products from vegetable oil industries. |
id |
RCAP_3ebfe43de9c7b6084b5e27860897c676 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/86024 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Olive and sunflower cakes as suitable substrates for lipase production by Yarrowia spp.: From flasks to bioreactorYarrowia lipolyticaLipaseSolid state fermentationScale upOlive and sunflower cakesSolid state fermentation is a biotechnological process that allows the utilization of agro-industrial by-products as solid substrates for the growth of microorganisms, including filamentous fungi and yeasts, with the simultaneous production of added value compounds, like enzymes. Oil cakes, such as olive and sunflower cakes, are generated during vegetable oil extraction and have received great interest as solid substrates in SSF processes. In the present work, a 50% (w/w) mixture of olive and sunflower cakes was used as substrate in SSF for lipase production by several strains of Y. lipolytica and Y. divulgata. The strain of Y. lipolytica W29 was the best lipase producer under small scale SSF and studies were conducted to address important factors in scaling-up the process. Different bioreactors were used and the effect of aeration and agitation on lipase production was studied. Higher lipase activity was obtained in tray bioreactors, reaching 85 U/g (dry mass of substrates) after 48h of SSF, while the operation in a horizontal drum bioreactor favored yeast growth, resulting in the highest yeast biomass production and a delay in lipase maximum activity. The scale-up of SSF for lipase production by Y. lipolytica has been successfully performed, showing that SSF is an interesting and eco-friendly bioprocessing strategy for the valorization of by-products from vegetable oil industries.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2020 and the PhD grant SFRH/BD/139098/2018 by LABBELS, Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020 and by the project BIOECONORTE (NORTE-01-0145-FEDER-000070).info:eu-repo/semantics/publishedVersionElsevier BVUniversidade do MinhoCosta, Ana RitaSalgado, José ManuelBelo, Isabel2023-082023-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/86024engCosta, A. R., Salgado, J. M., & Belo, I. (2023, August). Olive and sunflower cakes as suitable substrates for lipase production by Yarrowia spp.: From flasks to bioreactor. Biocatalysis and Agricultural Biotechnology. Elsevier BV. http://doi.org/10.1016/j.bcab.2023.1027831878-818110.1016/j.bcab.2023.102783102783https://www.sciencedirect.com/science/article/pii/S1878818123001846info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-12T01:18:18Zoai:repositorium.sdum.uminho.pt:1822/86024Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:26:40.261380Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Olive and sunflower cakes as suitable substrates for lipase production by Yarrowia spp.: From flasks to bioreactor |
title |
Olive and sunflower cakes as suitable substrates for lipase production by Yarrowia spp.: From flasks to bioreactor |
spellingShingle |
Olive and sunflower cakes as suitable substrates for lipase production by Yarrowia spp.: From flasks to bioreactor Costa, Ana Rita Yarrowia lipolytica Lipase Solid state fermentation Scale up Olive and sunflower cakes |
title_short |
Olive and sunflower cakes as suitable substrates for lipase production by Yarrowia spp.: From flasks to bioreactor |
title_full |
Olive and sunflower cakes as suitable substrates for lipase production by Yarrowia spp.: From flasks to bioreactor |
title_fullStr |
Olive and sunflower cakes as suitable substrates for lipase production by Yarrowia spp.: From flasks to bioreactor |
title_full_unstemmed |
Olive and sunflower cakes as suitable substrates for lipase production by Yarrowia spp.: From flasks to bioreactor |
title_sort |
Olive and sunflower cakes as suitable substrates for lipase production by Yarrowia spp.: From flasks to bioreactor |
author |
Costa, Ana Rita |
author_facet |
Costa, Ana Rita Salgado, José Manuel Belo, Isabel |
author_role |
author |
author2 |
Salgado, José Manuel Belo, Isabel |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Costa, Ana Rita Salgado, José Manuel Belo, Isabel |
dc.subject.por.fl_str_mv |
Yarrowia lipolytica Lipase Solid state fermentation Scale up Olive and sunflower cakes |
topic |
Yarrowia lipolytica Lipase Solid state fermentation Scale up Olive and sunflower cakes |
description |
Solid state fermentation is a biotechnological process that allows the utilization of agro-industrial by-products as solid substrates for the growth of microorganisms, including filamentous fungi and yeasts, with the simultaneous production of added value compounds, like enzymes. Oil cakes, such as olive and sunflower cakes, are generated during vegetable oil extraction and have received great interest as solid substrates in SSF processes. In the present work, a 50% (w/w) mixture of olive and sunflower cakes was used as substrate in SSF for lipase production by several strains of Y. lipolytica and Y. divulgata. The strain of Y. lipolytica W29 was the best lipase producer under small scale SSF and studies were conducted to address important factors in scaling-up the process. Different bioreactors were used and the effect of aeration and agitation on lipase production was studied. Higher lipase activity was obtained in tray bioreactors, reaching 85 U/g (dry mass of substrates) after 48h of SSF, while the operation in a horizontal drum bioreactor favored yeast growth, resulting in the highest yeast biomass production and a delay in lipase maximum activity. The scale-up of SSF for lipase production by Y. lipolytica has been successfully performed, showing that SSF is an interesting and eco-friendly bioprocessing strategy for the valorization of by-products from vegetable oil industries. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-08 2023-08-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/86024 |
url |
https://hdl.handle.net/1822/86024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Costa, A. R., Salgado, J. M., & Belo, I. (2023, August). Olive and sunflower cakes as suitable substrates for lipase production by Yarrowia spp.: From flasks to bioreactor. Biocatalysis and Agricultural Biotechnology. Elsevier BV. http://doi.org/10.1016/j.bcab.2023.102783 1878-8181 10.1016/j.bcab.2023.102783 102783 https://www.sciencedirect.com/science/article/pii/S1878818123001846 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier BV |
publisher.none.fl_str_mv |
Elsevier BV |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133534234869760 |