A regeneration-triggered metabolic adaptation is necessary for cell identity transitions and cell cycle re-entry to support blastema formation and bone regeneration

Detalhes bibliográficos
Autor(a) principal: Brandão, Ana S.
Data de Publicação: 2022
Outros Autores: Borbinha, Jorge, Pereira, Telmo, Brito, Patrícia H., Lourenço, Raquel, Bensimon-Brito, Anabela, Jacinto, Antonio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/143837
Resumo: Funding Funder Grant reference number Author Fundação para a Ciência e a Tecnologia PTDC/BIA-BID/29709/2017 Ana S Brandão Fundação para a Ciência e a Tecnologia PTDC/BTMSAL/29377/2017 Ana S Brandão Fundação para a Ciência e a Tecnologia UIDB/04462/2020 Ana S Brandão Fundação para a Ciência e a Tecnologia LA/P/0087/2020 Ana S Brandão Fundação para a Ciência e a Tecnologia LISBOA-01-0145- FEDER-022170 Ana S Brandão ATIP-Avenir Program Anabela Bensimon-Brito The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
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spelling A regeneration-triggered metabolic adaptation is necessary for cell identity transitions and cell cycle re-entry to support blastema formation and bone regenerationblastemacell fatecell metabolismdedifferentiationosteoblastregenerationregenerative medicinestem cellszebrafishNeuroscience(all)Biochemistry, Genetics and Molecular Biology(all)Immunology and Microbiology(all)Funding Funder Grant reference number Author Fundação para a Ciência e a Tecnologia PTDC/BIA-BID/29709/2017 Ana S Brandão Fundação para a Ciência e a Tecnologia PTDC/BTMSAL/29377/2017 Ana S Brandão Fundação para a Ciência e a Tecnologia UIDB/04462/2020 Ana S Brandão Fundação para a Ciência e a Tecnologia LA/P/0087/2020 Ana S Brandão Fundação para a Ciência e a Tecnologia LISBOA-01-0145- FEDER-022170 Ana S Brandão ATIP-Avenir Program Anabela Bensimon-Brito The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.Regeneration depends on the ability of mature cells at the injury site to respond to injury, generating tissue-specific progenitors that incorporate the blastema and proliferate to reconstitute the original organ architecture. The metabolic microenvironment has been tightly connected to cell function and identity during development and tumorigenesis. Yet, the link between metabolism and cell identity at the mechanistic level in a regenerative context remains unclear. The adult zebrafish caudal fin, and bone cells specifically, have been crucial for the understanding of mature cell contribution to tissue regeneration. Here, we use this model to explore the relevance of glucose metabolism for the cell fate transitions preceding new osteoblast formation and blastema assembly. We show that injury triggers a modulation in the metabolic profile at early stages of regeneration to enhance glycolysis at the expense of mitochondrial oxidation. This metabolic adaptation mediates transcriptional changes that make mature osteoblast amenable to be reprogramed into pre-osteoblasts and induces cell cycle re-entry and progression. Manipulation of the metabolic profile led to severe reduction of the pre-osteoblast pool, diminishing their capacity to generate new osteoblasts, and to a complete abrogation of blastema formation. Overall, our data indicate that metabolic alterations have a powerful instructive role in regulating genetic programs that dictate fate decisions and stimulate proliferation, thereby providing a deeper understanding on the mechanisms regulating blastema formation and bone regeneration.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)UCIBIO - Applied Molecular Biosciences UnitFaculdade de Ciências e Tecnologia (FCT)DCV - Departamento de Ciências da VidaRUNBrandão, Ana S.Borbinha, JorgePereira, TelmoBrito, Patrícia H.Lourenço, RaquelBensimon-Brito, AnabelaJacinto, Antonio2022-09-19T22:23:33Z2022-08-222022-08-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/143837eng2050-084XPURE: 46485845https://doi.org/10.7554/eLife.76987info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:22:36Zoai:run.unl.pt:10362/143837Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:51:09.776236Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A regeneration-triggered metabolic adaptation is necessary for cell identity transitions and cell cycle re-entry to support blastema formation and bone regeneration
title A regeneration-triggered metabolic adaptation is necessary for cell identity transitions and cell cycle re-entry to support blastema formation and bone regeneration
spellingShingle A regeneration-triggered metabolic adaptation is necessary for cell identity transitions and cell cycle re-entry to support blastema formation and bone regeneration
Brandão, Ana S.
blastema
cell fate
cell metabolism
dedifferentiation
osteoblast
regeneration
regenerative medicine
stem cells
zebrafish
Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
title_short A regeneration-triggered metabolic adaptation is necessary for cell identity transitions and cell cycle re-entry to support blastema formation and bone regeneration
title_full A regeneration-triggered metabolic adaptation is necessary for cell identity transitions and cell cycle re-entry to support blastema formation and bone regeneration
title_fullStr A regeneration-triggered metabolic adaptation is necessary for cell identity transitions and cell cycle re-entry to support blastema formation and bone regeneration
title_full_unstemmed A regeneration-triggered metabolic adaptation is necessary for cell identity transitions and cell cycle re-entry to support blastema formation and bone regeneration
title_sort A regeneration-triggered metabolic adaptation is necessary for cell identity transitions and cell cycle re-entry to support blastema formation and bone regeneration
author Brandão, Ana S.
author_facet Brandão, Ana S.
Borbinha, Jorge
Pereira, Telmo
Brito, Patrícia H.
Lourenço, Raquel
Bensimon-Brito, Anabela
Jacinto, Antonio
author_role author
author2 Borbinha, Jorge
Pereira, Telmo
Brito, Patrícia H.
Lourenço, Raquel
Bensimon-Brito, Anabela
Jacinto, Antonio
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Centro de Estudos de Doenças Crónicas (CEDOC)
UCIBIO - Applied Molecular Biosciences Unit
Faculdade de Ciências e Tecnologia (FCT)
DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Brandão, Ana S.
Borbinha, Jorge
Pereira, Telmo
Brito, Patrícia H.
Lourenço, Raquel
Bensimon-Brito, Anabela
Jacinto, Antonio
dc.subject.por.fl_str_mv blastema
cell fate
cell metabolism
dedifferentiation
osteoblast
regeneration
regenerative medicine
stem cells
zebrafish
Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
topic blastema
cell fate
cell metabolism
dedifferentiation
osteoblast
regeneration
regenerative medicine
stem cells
zebrafish
Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
description Funding Funder Grant reference number Author Fundação para a Ciência e a Tecnologia PTDC/BIA-BID/29709/2017 Ana S Brandão Fundação para a Ciência e a Tecnologia PTDC/BTMSAL/29377/2017 Ana S Brandão Fundação para a Ciência e a Tecnologia UIDB/04462/2020 Ana S Brandão Fundação para a Ciência e a Tecnologia LA/P/0087/2020 Ana S Brandão Fundação para a Ciência e a Tecnologia LISBOA-01-0145- FEDER-022170 Ana S Brandão ATIP-Avenir Program Anabela Bensimon-Brito The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-19T22:23:33Z
2022-08-22
2022-08-22T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/143837
url http://hdl.handle.net/10362/143837
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2050-084X
PURE: 46485845
https://doi.org/10.7554/eLife.76987
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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