In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.1371/journal.pone.0051766 |
Resumo: | Background: Zebrafish (Danio rerio) has a remarkable capacity to regenerate many organs and tissues. During larval stages the fin fold allows the possibility of performing long time-lapse imaging making this system very appealing to study the relationships between tissue movements, cell migration and proliferation necessary for the regeneration process. Results: Through the combined use of transgenic fluorescently-labeled animals and confocal microscopy imaging, we characterized in vivo the complete fin fold regeneration process. We show, for the first time, that there is an increase in the global rate of epidermal growth as a response to tissue loss. Also enhanced significantly is cell proliferation, which upon amputation happens in a broad area concerning the amputation level and not in a blastema-restricted way. This reveals a striking difference with regard to the adult fin regeneration system. Finally, an accumulation of migratory, shape-changing fibroblasts occurs proximally to the wound area, resembling a blastemal-like structure, which may act as a signaling center for the regeneration process to proceed. Conclusions: These findings provide a novel in vivo description of fundamental mechanisms occurring during the fin fold regeneration process, thereby contributing to a better knowledge of this regenerative system and to reveal variations in the epimorphic regeneration field. |
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In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold RegenerationAXOLOTL LIMB REGENERATIONFUNCTIONAL-ANALYSISBLASTEMA FORMATIONGENE-EXPRESSIONFUSION PROTEINTELEOST FINMORPHOGENESISDEDIFFERENTIATIONPROLIFERATIONGASTRULATIONMedicine(all)Biochemistry, Genetics and Molecular Biology(all)Agricultural and Biological Sciences(all)Background: Zebrafish (Danio rerio) has a remarkable capacity to regenerate many organs and tissues. During larval stages the fin fold allows the possibility of performing long time-lapse imaging making this system very appealing to study the relationships between tissue movements, cell migration and proliferation necessary for the regeneration process. Results: Through the combined use of transgenic fluorescently-labeled animals and confocal microscopy imaging, we characterized in vivo the complete fin fold regeneration process. We show, for the first time, that there is an increase in the global rate of epidermal growth as a response to tissue loss. Also enhanced significantly is cell proliferation, which upon amputation happens in a broad area concerning the amputation level and not in a blastema-restricted way. This reveals a striking difference with regard to the adult fin regeneration system. Finally, an accumulation of migratory, shape-changing fibroblasts occurs proximally to the wound area, resembling a blastemal-like structure, which may act as a signaling center for the regeneration process to proceed. Conclusions: These findings provide a novel in vivo description of fundamental mechanisms occurring during the fin fold regeneration process, thereby contributing to a better knowledge of this regenerative system and to reveal variations in the epimorphic regeneration field.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNMateus, RitaPereira, TelmoSousa, Sarade Lima, Joana EstevesPascoal, SusanaSaúde, LeonorJacinto, Antonio2017-08-03T22:05:04Z2012-12-202012-12-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1371/journal.pone.0051766eng1932-6203PURE: 2991045http://www.scopus.com/inward/record.url?scp=84871414741&partnerID=8YFLogxKhttps://doi.org/10.1371/journal.pone.0051766info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:10:08Zoai:run.unl.pt:10362/22459Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:27:18.749376Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration |
title |
In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration |
spellingShingle |
In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration Mateus, Rita AXOLOTL LIMB REGENERATION FUNCTIONAL-ANALYSIS BLASTEMA FORMATION GENE-EXPRESSION FUSION PROTEIN TELEOST FIN MORPHOGENESIS DEDIFFERENTIATION PROLIFERATION GASTRULATION Medicine(all) Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) |
title_short |
In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration |
title_full |
In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration |
title_fullStr |
In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration |
title_full_unstemmed |
In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration |
title_sort |
In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration |
author |
Mateus, Rita |
author_facet |
Mateus, Rita Pereira, Telmo Sousa, Sara de Lima, Joana Esteves Pascoal, Susana Saúde, Leonor Jacinto, Antonio |
author_role |
author |
author2 |
Pereira, Telmo Sousa, Sara de Lima, Joana Esteves Pascoal, Susana Saúde, Leonor Jacinto, Antonio |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Centro de Estudos de Doenças Crónicas (CEDOC) NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) RUN |
dc.contributor.author.fl_str_mv |
Mateus, Rita Pereira, Telmo Sousa, Sara de Lima, Joana Esteves Pascoal, Susana Saúde, Leonor Jacinto, Antonio |
dc.subject.por.fl_str_mv |
AXOLOTL LIMB REGENERATION FUNCTIONAL-ANALYSIS BLASTEMA FORMATION GENE-EXPRESSION FUSION PROTEIN TELEOST FIN MORPHOGENESIS DEDIFFERENTIATION PROLIFERATION GASTRULATION Medicine(all) Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) |
topic |
AXOLOTL LIMB REGENERATION FUNCTIONAL-ANALYSIS BLASTEMA FORMATION GENE-EXPRESSION FUSION PROTEIN TELEOST FIN MORPHOGENESIS DEDIFFERENTIATION PROLIFERATION GASTRULATION Medicine(all) Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) |
description |
Background: Zebrafish (Danio rerio) has a remarkable capacity to regenerate many organs and tissues. During larval stages the fin fold allows the possibility of performing long time-lapse imaging making this system very appealing to study the relationships between tissue movements, cell migration and proliferation necessary for the regeneration process. Results: Through the combined use of transgenic fluorescently-labeled animals and confocal microscopy imaging, we characterized in vivo the complete fin fold regeneration process. We show, for the first time, that there is an increase in the global rate of epidermal growth as a response to tissue loss. Also enhanced significantly is cell proliferation, which upon amputation happens in a broad area concerning the amputation level and not in a blastema-restricted way. This reveals a striking difference with regard to the adult fin regeneration system. Finally, an accumulation of migratory, shape-changing fibroblasts occurs proximally to the wound area, resembling a blastemal-like structure, which may act as a signaling center for the regeneration process to proceed. Conclusions: These findings provide a novel in vivo description of fundamental mechanisms occurring during the fin fold regeneration process, thereby contributing to a better knowledge of this regenerative system and to reveal variations in the epimorphic regeneration field. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-20 2012-12-20T00:00:00Z 2017-08-03T22:05:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1371/journal.pone.0051766 |
url |
https://doi.org/10.1371/journal.pone.0051766 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1932-6203 PURE: 2991045 http://www.scopus.com/inward/record.url?scp=84871414741&partnerID=8YFLogxK https://doi.org/10.1371/journal.pone.0051766 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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