Ectopic expression of Kip-related proteins restrains root-knot nematode-feeding site expansion
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/10260 https://doi.org/10.1111/nph.12255 |
Resumo: | The development of nematode feeding sites induced by root-knot nematodes involves the synchronized activation of cell cycle processes such as acytokinetic mitoses and DNA amplification. A number of key cell cycle genes are reported to be critical for nematode feeding site development. However, it remains unknown whether plant cyclin-dependent kinase (CDK) inhibitors such as the Arabidopsis interactor/inhibitor of CDK (ICK)/Kip-related protein (KRP) family are involved in nematode feeding site development. This study demonstrates the involvement of Arabidopsis ICK2/KRP2 and ICK1/KRP1 in the control of mitosis to endoreduplication in galls induced by the root-knot nematode Meloidogyne incognita. ! Using ICK/KRP promoter-GUS fusions and mRNA in situ hybridizations, we showed that ICK2/KRP2, ICK3/KRP5 and ICK4/KRP6 are expressed in galls after nematode infection. Loss-of-function mutants have minor effects on gall development and nematode reproduction. Conversely, overexpression of both ICK1/KRP1 and ICK2/KRP2 impaired mitosis in giant cells and blocked neighboring cell proliferation, resulting in a drastic reduction of gall size. ! Studying the dynamics of protein expression demonstrated that protein levels of ICK2/ KRP2 are tightly regulated during giant cell development and reliant on the presence of the nematode. ! This work demonstrates that impeding cell cycle progression by means of ICK1/KRP1 and ICK2/KRP2 overexpression severely restricts gall development, leading to a marked limitation of root-knot nematode development and reduced numbers of offspring |
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Ectopic expression of Kip-related proteins restrains root-knot nematode-feeding site expansionArabidopsis roots, cell cycleMeloidogyneThe development of nematode feeding sites induced by root-knot nematodes involves the synchronized activation of cell cycle processes such as acytokinetic mitoses and DNA amplification. A number of key cell cycle genes are reported to be critical for nematode feeding site development. However, it remains unknown whether plant cyclin-dependent kinase (CDK) inhibitors such as the Arabidopsis interactor/inhibitor of CDK (ICK)/Kip-related protein (KRP) family are involved in nematode feeding site development. This study demonstrates the involvement of Arabidopsis ICK2/KRP2 and ICK1/KRP1 in the control of mitosis to endoreduplication in galls induced by the root-knot nematode Meloidogyne incognita. ! Using ICK/KRP promoter-GUS fusions and mRNA in situ hybridizations, we showed that ICK2/KRP2, ICK3/KRP5 and ICK4/KRP6 are expressed in galls after nematode infection. Loss-of-function mutants have minor effects on gall development and nematode reproduction. Conversely, overexpression of both ICK1/KRP1 and ICK2/KRP2 impaired mitosis in giant cells and blocked neighboring cell proliferation, resulting in a drastic reduction of gall size. ! Studying the dynamics of protein expression demonstrated that protein levels of ICK2/ KRP2 are tightly regulated during giant cell development and reliant on the presence of the nematode. ! This work demonstrates that impeding cell cycle progression by means of ICK1/KRP1 and ICK2/KRP2 overexpression severely restricts gall development, leading to a marked limitation of root-knot nematode development and reduced numbers of offspringNew Phytologist Trust2014-01-29T13:03:53Z2014-01-292013-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/10260http://hdl.handle.net/10174/10260https://doi.org/10.1111/nph.12255engNew Phytologistndndndndndndmmota@uevora.ptndndndjanice.almeida-engler@sophia.inra.fr211Vieira, PauloEscudero, CarmenRodiuc, NataliaBoruc, JoanaRussinova, EugeniaGlab, NathalieMota, ManuelVeylder, LievenAbad, PierreEngler, GilbertAlmeida Engler, Janiceinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:53:00Zoai:dspace.uevora.pt:10174/10260Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:04:12.349117Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Ectopic expression of Kip-related proteins restrains root-knot nematode-feeding site expansion |
title |
Ectopic expression of Kip-related proteins restrains root-knot nematode-feeding site expansion |
spellingShingle |
Ectopic expression of Kip-related proteins restrains root-knot nematode-feeding site expansion Vieira, Paulo Arabidopsis roots, cell cycle Meloidogyne |
title_short |
Ectopic expression of Kip-related proteins restrains root-knot nematode-feeding site expansion |
title_full |
Ectopic expression of Kip-related proteins restrains root-knot nematode-feeding site expansion |
title_fullStr |
Ectopic expression of Kip-related proteins restrains root-knot nematode-feeding site expansion |
title_full_unstemmed |
Ectopic expression of Kip-related proteins restrains root-knot nematode-feeding site expansion |
title_sort |
Ectopic expression of Kip-related proteins restrains root-knot nematode-feeding site expansion |
author |
Vieira, Paulo |
author_facet |
Vieira, Paulo Escudero, Carmen Rodiuc, Natalia Boruc, Joana Russinova, Eugenia Glab, Nathalie Mota, Manuel Veylder, Lieven Abad, Pierre Engler, Gilbert Almeida Engler, Janice |
author_role |
author |
author2 |
Escudero, Carmen Rodiuc, Natalia Boruc, Joana Russinova, Eugenia Glab, Nathalie Mota, Manuel Veylder, Lieven Abad, Pierre Engler, Gilbert Almeida Engler, Janice |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Vieira, Paulo Escudero, Carmen Rodiuc, Natalia Boruc, Joana Russinova, Eugenia Glab, Nathalie Mota, Manuel Veylder, Lieven Abad, Pierre Engler, Gilbert Almeida Engler, Janice |
dc.subject.por.fl_str_mv |
Arabidopsis roots, cell cycle Meloidogyne |
topic |
Arabidopsis roots, cell cycle Meloidogyne |
description |
The development of nematode feeding sites induced by root-knot nematodes involves the synchronized activation of cell cycle processes such as acytokinetic mitoses and DNA amplification. A number of key cell cycle genes are reported to be critical for nematode feeding site development. However, it remains unknown whether plant cyclin-dependent kinase (CDK) inhibitors such as the Arabidopsis interactor/inhibitor of CDK (ICK)/Kip-related protein (KRP) family are involved in nematode feeding site development. This study demonstrates the involvement of Arabidopsis ICK2/KRP2 and ICK1/KRP1 in the control of mitosis to endoreduplication in galls induced by the root-knot nematode Meloidogyne incognita. ! Using ICK/KRP promoter-GUS fusions and mRNA in situ hybridizations, we showed that ICK2/KRP2, ICK3/KRP5 and ICK4/KRP6 are expressed in galls after nematode infection. Loss-of-function mutants have minor effects on gall development and nematode reproduction. Conversely, overexpression of both ICK1/KRP1 and ICK2/KRP2 impaired mitosis in giant cells and blocked neighboring cell proliferation, resulting in a drastic reduction of gall size. ! Studying the dynamics of protein expression demonstrated that protein levels of ICK2/ KRP2 are tightly regulated during giant cell development and reliant on the presence of the nematode. ! This work demonstrates that impeding cell cycle progression by means of ICK1/KRP1 and ICK2/KRP2 overexpression severely restricts gall development, leading to a marked limitation of root-knot nematode development and reduced numbers of offspring |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-01T00:00:00Z 2014-01-29T13:03:53Z 2014-01-29 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/10260 http://hdl.handle.net/10174/10260 https://doi.org/10.1111/nph.12255 |
url |
http://hdl.handle.net/10174/10260 https://doi.org/10.1111/nph.12255 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
New Phytologist nd nd nd nd nd nd mmota@uevora.pt nd nd nd janice.almeida-engler@sophia.inra.fr 211 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
New Phytologist Trust |
publisher.none.fl_str_mv |
New Phytologist Trust |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136526345437184 |