Public health surveillance of multidrug-resistant clones of Neisseria gonorrhoeae in Europe: a genomic survey
| Autor(a) principal: | |
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| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.18/6243 |
Resumo: | Euro-GASP study group: INSA - Maria José Borrego |
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Public health surveillance of multidrug-resistant clones of Neisseria gonorrhoeae in Europe: a genomic surveyNeisseria gonorrhoeaePublic Health SurveillanceMultidrug-resistantsEuropeGenomic SurveyInfecções Sexualmente TransmissíveisEuro-GASP study group: INSA - Maria José BorregoFree PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6010626/Background: Traditional methods for molecular epidemiology of Neisseria gonorrhoeae are suboptimal. Whole-genome sequencing (WGS) offers ideal resolution to describe population dynamics and to predict and infer transmission of antimicrobial resistance, and can enhance infection control through linkage with epidemiological data. We used WGS, in conjunction with linked epidemiological and phenotypic data, to describe the gonococcal population in 20 European countries. We aimed to detail changes in phenotypic antimicrobial resistance levels (and the reasons for these changes) and strain distribution (with a focus on antimicrobial resistance strains in risk groups), and to predict antimicrobial resistance from WGS data. Methods: We carried out an observational study, in which we sequenced isolates taken from patients with gonorrhoea from the European Gonococcal Antimicrobial Surveillance Programme in 20 countries from September to November, 2013. We also developed a web platform that we used for automated antimicrobial resistance prediction, molecular typing (N gonorrhoeae multi-antigen sequence typing [NG-MAST] and multilocus sequence typing), and phylogenetic clustering in conjunction with epidemiological and phenotypic data. Findings: The multidrug-resistant NG-MAST genogroup G1407 was predominant and accounted for the most cephalosporin resistance, but the prevalence of this genogroup decreased from 248 (23%) of 1066 isolates in a previous study from 2009–10 to 174 (17%) of 1054 isolates in this survey in 2013. This genogroup previously showed an association with men who have sex with men, but changed to an association with heterosexual people (odds ratio=4·29). WGS provided substantially improved resolution and accuracy over NG-MAST and multilocus sequence typing, predicted antimicrobial resistance relatively well, and identified discrepant isolates, mixed infections or contaminants, and multidrug-resistant clades linked to risk groups. Interpretation: To our knowledge, we provide the first use of joint analysis of WGS and epidemiological data in an international programme for regional surveillance of sexually transmitted infections. WGS provided enhanced understanding of the distribution of antimicrobial resistance clones, including replacement with clones that were more susceptible to antimicrobials, in several risk groups nationally and regionally. We provide a framework for genomic surveillance of gonococci through standardised sampling, use of WGS, and a shared information architecture for interpretation and dissemination by use of open access software.The European Centre for Disease Prevention and Control, The Centre for Genomic Pathogen Surveillance, Örebro University Hospital, and Wellcome.Elsevier/ LancetRepositório Científico do Instituto Nacional de SaúdeHarris, Simon R.Cole, Michelle J.Spiteri, GianfrancoSánchez-Busó, LeonorGolparian, DanielJacobsson, SusanneGoater, RichardAbudahab, KhalilYeats, Corin A.Bercot, BeatriceBorrego, Maria JoséCrowley, BrendanStefanelli, PaolaTripodo, FrancescoAbad, RaquelAanensen, David M.Unemo, MagnusEuro-GASP study group2019-03-20T15:19:29Z2018-072018-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6243engLancet Infect Dis. 2018 Jul;18(7):758-768. doi: 10.1016/S1473-3099(18)30225-1. Epub 2018 May 151473-309910.1016/S1473-3099(18)30225-1info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:19Zoai:repositorio.insa.pt:10400.18/6243Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:40:55.321474Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Public health surveillance of multidrug-resistant clones of Neisseria gonorrhoeae in Europe: a genomic survey |
| title |
Public health surveillance of multidrug-resistant clones of Neisseria gonorrhoeae in Europe: a genomic survey |
| spellingShingle |
Public health surveillance of multidrug-resistant clones of Neisseria gonorrhoeae in Europe: a genomic survey Harris, Simon R. Neisseria gonorrhoeae Public Health Surveillance Multidrug-resistants Europe Genomic Survey Infecções Sexualmente Transmissíveis |
| title_short |
Public health surveillance of multidrug-resistant clones of Neisseria gonorrhoeae in Europe: a genomic survey |
| title_full |
Public health surveillance of multidrug-resistant clones of Neisseria gonorrhoeae in Europe: a genomic survey |
| title_fullStr |
Public health surveillance of multidrug-resistant clones of Neisseria gonorrhoeae in Europe: a genomic survey |
| title_full_unstemmed |
Public health surveillance of multidrug-resistant clones of Neisseria gonorrhoeae in Europe: a genomic survey |
| title_sort |
Public health surveillance of multidrug-resistant clones of Neisseria gonorrhoeae in Europe: a genomic survey |
| author |
Harris, Simon R. |
| author_facet |
Harris, Simon R. Cole, Michelle J. Spiteri, Gianfranco Sánchez-Busó, Leonor Golparian, Daniel Jacobsson, Susanne Goater, Richard Abudahab, Khalil Yeats, Corin A. Bercot, Beatrice Borrego, Maria José Crowley, Brendan Stefanelli, Paola Tripodo, Francesco Abad, Raquel Aanensen, David M. Unemo, Magnus Euro-GASP study group |
| author_role |
author |
| author2 |
Cole, Michelle J. Spiteri, Gianfranco Sánchez-Busó, Leonor Golparian, Daniel Jacobsson, Susanne Goater, Richard Abudahab, Khalil Yeats, Corin A. Bercot, Beatrice Borrego, Maria José Crowley, Brendan Stefanelli, Paola Tripodo, Francesco Abad, Raquel Aanensen, David M. Unemo, Magnus Euro-GASP study group |
| author2_role |
author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
| dc.contributor.author.fl_str_mv |
Harris, Simon R. Cole, Michelle J. Spiteri, Gianfranco Sánchez-Busó, Leonor Golparian, Daniel Jacobsson, Susanne Goater, Richard Abudahab, Khalil Yeats, Corin A. Bercot, Beatrice Borrego, Maria José Crowley, Brendan Stefanelli, Paola Tripodo, Francesco Abad, Raquel Aanensen, David M. Unemo, Magnus Euro-GASP study group |
| dc.subject.por.fl_str_mv |
Neisseria gonorrhoeae Public Health Surveillance Multidrug-resistants Europe Genomic Survey Infecções Sexualmente Transmissíveis |
| topic |
Neisseria gonorrhoeae Public Health Surveillance Multidrug-resistants Europe Genomic Survey Infecções Sexualmente Transmissíveis |
| description |
Euro-GASP study group: INSA - Maria José Borrego |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-07 2018-07-01T00:00:00Z 2019-03-20T15:19:29Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/6243 |
| url |
http://hdl.handle.net/10400.18/6243 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Lancet Infect Dis. 2018 Jul;18(7):758-768. doi: 10.1016/S1473-3099(18)30225-1. Epub 2018 May 15 1473-3099 10.1016/S1473-3099(18)30225-1 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
| eu_rights_str_mv |
embargoedAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier/ Lancet |
| publisher.none.fl_str_mv |
Elsevier/ Lancet |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132151944314880 |