Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry

Detalhes bibliográficos
Autor(a) principal: Silva, Ricardo M. P. da
Data de Publicação: 2008
Outros Autores: Mano, J. F., Reis, R. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/20269
Resumo: First-derivative UV spectrophotometry is shown to be a reliable method for the determination of the degree of N-acetylation of chitosan samples. A mathematical expression is derived that allows to determine the DA directly from the mass concentration of a chitosan solution and the first derivative of its UV spectrum at 202 nm, thus eliminating the need for empiric correction curves for highly deacetylated samples. A procedure is proposed for the accurate mass determination of the hygroscopic chitosan. The proposed approach facilitates the routine determination of the DA, especially when using potent multiwell microplate readers, which allow hundreds of samples to be measured in just a few minutes.
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spelling Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometryacetylationchitinchitosandeacetylationNMRUV spectrophotometryScience & TechnologyFirst-derivative UV spectrophotometry is shown to be a reliable method for the determination of the degree of N-acetylation of chitosan samples. A mathematical expression is derived that allows to determine the DA directly from the mass concentration of a chitosan solution and the first derivative of its UV spectrum at 202 nm, thus eliminating the need for empiric correction curves for highly deacetylated samples. A procedure is proposed for the accurate mass determination of the hygroscopic chitosan. The proposed approach facilitates the routine determination of the DA, especially when using potent multiwell microplate readers, which allow hundreds of samples to be measured in just a few minutes.WileyUniversidade do MinhoSilva, Ricardo M. P. daMano, J. F.Reis, R. L.20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/20269eng1521-393110.1002/macp.200800191info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:44:12Zoai:repositorium.sdum.uminho.pt:1822/20269Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:41:50.610002Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry
title Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry
spellingShingle Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry
Silva, Ricardo M. P. da
acetylation
chitin
chitosan
deacetylation
NMR
UV spectrophotometry
Science & Technology
title_short Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry
title_full Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry
title_fullStr Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry
title_full_unstemmed Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry
title_sort Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry
author Silva, Ricardo M. P. da
author_facet Silva, Ricardo M. P. da
Mano, J. F.
Reis, R. L.
author_role author
author2 Mano, J. F.
Reis, R. L.
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva, Ricardo M. P. da
Mano, J. F.
Reis, R. L.
dc.subject.por.fl_str_mv acetylation
chitin
chitosan
deacetylation
NMR
UV spectrophotometry
Science & Technology
topic acetylation
chitin
chitosan
deacetylation
NMR
UV spectrophotometry
Science & Technology
description First-derivative UV spectrophotometry is shown to be a reliable method for the determination of the degree of N-acetylation of chitosan samples. A mathematical expression is derived that allows to determine the DA directly from the mass concentration of a chitosan solution and the first derivative of its UV spectrum at 202 nm, thus eliminating the need for empiric correction curves for highly deacetylated samples. A procedure is proposed for the accurate mass determination of the hygroscopic chitosan. The proposed approach facilitates the routine determination of the DA, especially when using potent multiwell microplate readers, which allow hundreds of samples to be measured in just a few minutes.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/20269
url http://hdl.handle.net/1822/20269
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1521-3931
10.1002/macp.200800191
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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