Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/20269 |
Resumo: | First-derivative UV spectrophotometry is shown to be a reliable method for the determination of the degree of N-acetylation of chitosan samples. A mathematical expression is derived that allows to determine the DA directly from the mass concentration of a chitosan solution and the first derivative of its UV spectrum at 202 nm, thus eliminating the need for empiric correction curves for highly deacetylated samples. A procedure is proposed for the accurate mass determination of the hygroscopic chitosan. The proposed approach facilitates the routine determination of the DA, especially when using potent multiwell microplate readers, which allow hundreds of samples to be measured in just a few minutes. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometryacetylationchitinchitosandeacetylationNMRUV spectrophotometryScience & TechnologyFirst-derivative UV spectrophotometry is shown to be a reliable method for the determination of the degree of N-acetylation of chitosan samples. A mathematical expression is derived that allows to determine the DA directly from the mass concentration of a chitosan solution and the first derivative of its UV spectrum at 202 nm, thus eliminating the need for empiric correction curves for highly deacetylated samples. A procedure is proposed for the accurate mass determination of the hygroscopic chitosan. The proposed approach facilitates the routine determination of the DA, especially when using potent multiwell microplate readers, which allow hundreds of samples to be measured in just a few minutes.WileyUniversidade do MinhoSilva, Ricardo M. P. daMano, J. F.Reis, R. L.20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/20269eng1521-393110.1002/macp.200800191info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:44:12Zoai:repositorium.sdum.uminho.pt:1822/20269Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:41:50.610002Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry |
title |
Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry |
spellingShingle |
Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry Silva, Ricardo M. P. da acetylation chitin chitosan deacetylation NMR UV spectrophotometry Science & Technology |
title_short |
Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry |
title_full |
Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry |
title_fullStr |
Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry |
title_full_unstemmed |
Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry |
title_sort |
Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry |
author |
Silva, Ricardo M. P. da |
author_facet |
Silva, Ricardo M. P. da Mano, J. F. Reis, R. L. |
author_role |
author |
author2 |
Mano, J. F. Reis, R. L. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Silva, Ricardo M. P. da Mano, J. F. Reis, R. L. |
dc.subject.por.fl_str_mv |
acetylation chitin chitosan deacetylation NMR UV spectrophotometry Science & Technology |
topic |
acetylation chitin chitosan deacetylation NMR UV spectrophotometry Science & Technology |
description |
First-derivative UV spectrophotometry is shown to be a reliable method for the determination of the degree of N-acetylation of chitosan samples. A mathematical expression is derived that allows to determine the DA directly from the mass concentration of a chitosan solution and the first derivative of its UV spectrum at 202 nm, thus eliminating the need for empiric correction curves for highly deacetylated samples. A procedure is proposed for the accurate mass determination of the hygroscopic chitosan. The proposed approach facilitates the routine determination of the DA, especially when using potent multiwell microplate readers, which allow hundreds of samples to be measured in just a few minutes. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2008-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/20269 |
url |
http://hdl.handle.net/1822/20269 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1521-3931 10.1002/macp.200800191 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799132969258975232 |