Bovine lactoferrin-loaded plasmonic magnetoliposomes for antifungal therapeutic applications
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/86591 |
Resumo: | Bovine lactoferrin (bLf) is a milk-derived protein that exhibits potent broad-spectrum antifungal activity against multiple fungi. bLf is susceptible to degradation, while some of its properties depend on the tertiary structure. So, the encapsulation of bLf in stimuli-responsive therapeutic formulations provides an added value to enhance its biological activities. Plasmonic magnetoliposomes (PMLs) arise as promising nanocarriers for dual hyperthermia (magneto-photothermia) and local chemotherapy, since the combination of magnetic and gold nanoparticles (NPs) in a single nanosystem (multifunctional liposomes) enables the targeting and controlled release of loaded drugs. In this work, plasmonic magnetoliposomes (PMLs) containing manganese ferrite nanoparticles (28 nm size) and gold nanoparticles (5-7.5 nm size), functionalized with 11-mercaptoundecanoic acid or octadecanethiol, were prepared and loaded with bLf. The NPs' optical, magnetic and structural properties were measured via UV/vis/NIR absorption spectroscopy, SQUID and TEM, respectively. The Specific Absorption Rate (SAR) was calculated to assess the capabilities for magnetic and photothermal hyperthermia. Finally, the antifungal potential of bLf-loaded PMLs and their mechanism of internalization were assessed in Saccharomyces cerevisiae by counting the colony forming units and using fluorescence microscopy. The results demonstrate that PMLs are mainly internalized through an energy- and temperature-dependent endocytic process, though the contribution of a diffusion component cannot be discarded. Most notably, only bLf-loaded plasmonic magnetoliposomes display cytotoxicity with an efficiency similar to free bLf, attesting their promising potential for bLf delivery in the context of antifungal therapeutic interventions. |
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Bovine lactoferrin-loaded plasmonic magnetoliposomes for antifungal therapeutic applicationsbovine lactoferrinplasmonic magnetoliposomesdual hyperthermiaantifungal activitycytotoxicityCiências Médicas::Biotecnologia MédicaCiências Naturais::Ciências FísicasCiências Naturais::Ciências BiológicasEngenharia e Tecnologia::NanotecnologiaSaúde de qualidadeProdução e consumo sustentáveisBovine lactoferrin (bLf) is a milk-derived protein that exhibits potent broad-spectrum antifungal activity against multiple fungi. bLf is susceptible to degradation, while some of its properties depend on the tertiary structure. So, the encapsulation of bLf in stimuli-responsive therapeutic formulations provides an added value to enhance its biological activities. Plasmonic magnetoliposomes (PMLs) arise as promising nanocarriers for dual hyperthermia (magneto-photothermia) and local chemotherapy, since the combination of magnetic and gold nanoparticles (NPs) in a single nanosystem (multifunctional liposomes) enables the targeting and controlled release of loaded drugs. In this work, plasmonic magnetoliposomes (PMLs) containing manganese ferrite nanoparticles (28 nm size) and gold nanoparticles (5-7.5 nm size), functionalized with 11-mercaptoundecanoic acid or octadecanethiol, were prepared and loaded with bLf. The NPs' optical, magnetic and structural properties were measured via UV/vis/NIR absorption spectroscopy, SQUID and TEM, respectively. The Specific Absorption Rate (SAR) was calculated to assess the capabilities for magnetic and photothermal hyperthermia. Finally, the antifungal potential of bLf-loaded PMLs and their mechanism of internalization were assessed in Saccharomyces cerevisiae by counting the colony forming units and using fluorescence microscopy. The results demonstrate that PMLs are mainly internalized through an energy- and temperature-dependent endocytic process, though the contribution of a diffusion component cannot be discarded. Most notably, only bLf-loaded plasmonic magnetoliposomes display cytotoxicity with an efficiency similar to free bLf, attesting their promising potential for bLf delivery in the context of antifungal therapeutic interventions.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of CEB (UIDB/04469/2020), CBMA (UIDB/04050/2020) and CF-UM-UP (UIDB/04650/2020) units.MDPIUniversidade do MinhoPereira, MélanieRodrigues, Ana Rita OliveiraAmaral, LeslieCôrte-Real, ManuelaPereira, Cátia Sofia SantosCastanheira, Elisabete M. S.2023-08-192023-08-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/86591engPereira, M.; Rodrigues, A.R.O.; Amaral, L.; Côrte-Real, M.; Santos-Pereira, C.; Castanheira, E.M.S. Bovine Lactoferrin-Loaded Plasmonic Magnetoliposomes for Antifungal Therapeutic Applications. Pharmaceutics 2023, 15, 2162. https://doi.org/10.3390/pharmaceutics150821621999-492310.3390/pharmaceutics15082162376313762162https://www.mdpi.com/1999-4923/15/8/2162info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-09-30T01:33:01Zoai:repositorium.sdum.uminho.pt:1822/86591Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:31:46.085350Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bovine lactoferrin-loaded plasmonic magnetoliposomes for antifungal therapeutic applications |
title |
Bovine lactoferrin-loaded plasmonic magnetoliposomes for antifungal therapeutic applications |
spellingShingle |
Bovine lactoferrin-loaded plasmonic magnetoliposomes for antifungal therapeutic applications Pereira, Mélanie bovine lactoferrin plasmonic magnetoliposomes dual hyperthermia antifungal activity cytotoxicity Ciências Médicas::Biotecnologia Médica Ciências Naturais::Ciências Físicas Ciências Naturais::Ciências Biológicas Engenharia e Tecnologia::Nanotecnologia Saúde de qualidade Produção e consumo sustentáveis |
title_short |
Bovine lactoferrin-loaded plasmonic magnetoliposomes for antifungal therapeutic applications |
title_full |
Bovine lactoferrin-loaded plasmonic magnetoliposomes for antifungal therapeutic applications |
title_fullStr |
Bovine lactoferrin-loaded plasmonic magnetoliposomes for antifungal therapeutic applications |
title_full_unstemmed |
Bovine lactoferrin-loaded plasmonic magnetoliposomes for antifungal therapeutic applications |
title_sort |
Bovine lactoferrin-loaded plasmonic magnetoliposomes for antifungal therapeutic applications |
author |
Pereira, Mélanie |
author_facet |
Pereira, Mélanie Rodrigues, Ana Rita Oliveira Amaral, Leslie Côrte-Real, Manuela Pereira, Cátia Sofia Santos Castanheira, Elisabete M. S. |
author_role |
author |
author2 |
Rodrigues, Ana Rita Oliveira Amaral, Leslie Côrte-Real, Manuela Pereira, Cátia Sofia Santos Castanheira, Elisabete M. S. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pereira, Mélanie Rodrigues, Ana Rita Oliveira Amaral, Leslie Côrte-Real, Manuela Pereira, Cátia Sofia Santos Castanheira, Elisabete M. S. |
dc.subject.por.fl_str_mv |
bovine lactoferrin plasmonic magnetoliposomes dual hyperthermia antifungal activity cytotoxicity Ciências Médicas::Biotecnologia Médica Ciências Naturais::Ciências Físicas Ciências Naturais::Ciências Biológicas Engenharia e Tecnologia::Nanotecnologia Saúde de qualidade Produção e consumo sustentáveis |
topic |
bovine lactoferrin plasmonic magnetoliposomes dual hyperthermia antifungal activity cytotoxicity Ciências Médicas::Biotecnologia Médica Ciências Naturais::Ciências Físicas Ciências Naturais::Ciências Biológicas Engenharia e Tecnologia::Nanotecnologia Saúde de qualidade Produção e consumo sustentáveis |
description |
Bovine lactoferrin (bLf) is a milk-derived protein that exhibits potent broad-spectrum antifungal activity against multiple fungi. bLf is susceptible to degradation, while some of its properties depend on the tertiary structure. So, the encapsulation of bLf in stimuli-responsive therapeutic formulations provides an added value to enhance its biological activities. Plasmonic magnetoliposomes (PMLs) arise as promising nanocarriers for dual hyperthermia (magneto-photothermia) and local chemotherapy, since the combination of magnetic and gold nanoparticles (NPs) in a single nanosystem (multifunctional liposomes) enables the targeting and controlled release of loaded drugs. In this work, plasmonic magnetoliposomes (PMLs) containing manganese ferrite nanoparticles (28 nm size) and gold nanoparticles (5-7.5 nm size), functionalized with 11-mercaptoundecanoic acid or octadecanethiol, were prepared and loaded with bLf. The NPs' optical, magnetic and structural properties were measured via UV/vis/NIR absorption spectroscopy, SQUID and TEM, respectively. The Specific Absorption Rate (SAR) was calculated to assess the capabilities for magnetic and photothermal hyperthermia. Finally, the antifungal potential of bLf-loaded PMLs and their mechanism of internalization were assessed in Saccharomyces cerevisiae by counting the colony forming units and using fluorescence microscopy. The results demonstrate that PMLs are mainly internalized through an energy- and temperature-dependent endocytic process, though the contribution of a diffusion component cannot be discarded. Most notably, only bLf-loaded plasmonic magnetoliposomes display cytotoxicity with an efficiency similar to free bLf, attesting their promising potential for bLf delivery in the context of antifungal therapeutic interventions. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-08-19 2023-08-19T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/86591 |
url |
https://hdl.handle.net/1822/86591 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pereira, M.; Rodrigues, A.R.O.; Amaral, L.; Côrte-Real, M.; Santos-Pereira, C.; Castanheira, E.M.S. Bovine Lactoferrin-Loaded Plasmonic Magnetoliposomes for Antifungal Therapeutic Applications. Pharmaceutics 2023, 15, 2162. https://doi.org/10.3390/pharmaceutics15082162 1999-4923 10.3390/pharmaceutics15082162 37631376 2162 https://www.mdpi.com/1999-4923/15/8/2162 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133585901355008 |