Systematic structure-based search for ochratoxin-degrading enzymes in proteomes from filamentous fungi

Detalhes bibliográficos
Autor(a) principal: Leitão, Ana Lúcia
Data de Publicação: 2021
Outros Autores: Enguita, Francisco J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/135182
Resumo: PTDC-MED-GEN-29389-2017
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network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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spelling Systematic structure-based search for ochratoxin-degrading enzymes in proteomes from filamentous fungiBiodegradationMycotoxinOchratoxin AOchratoxinaseStructural biologyBiochemistryMolecular BiologyPTDC-MED-GEN-29389-2017(1) Background: ochratoxins are mycotoxins produced by filamentous fungi with important implications in the food manufacturing industry due to their toxicity. Decontamination by specific ochratoxin-degrading enzymes has become an interesting alternative for the treatment of contaminated food commodities. (2) Methods: using a structure-based approach based on homology modeling, blind molecular docking of substrates and characterization of low-frequency protein motions, we performed a proteome mining in filamentous fungi to characterize new enzymes with potential ochratoxinase activity. (3) Results: the proteome mining results demonstrated the ubiquitous presence of fungal binuclear zinc-dependent amido-hydrolases with a high degree of structural homology to the already characterized ochratoxinase from Aspergillus niger. Ochratoxinase-like enzymes from ochratoxin-producing fungi showed more favorable substrate-binding pockets to accommodate ochratoxins A and B. (4) Conclusions: filamentous fungi are an interesting and rich source of hydrolases potentially capable of degrading ochratoxins, and could be used for the detoxification of diverse food commodities.MEtRICS - Centro de Engenharia Mecânica e Sustentabilidade de RecursosDCTB - Departamento de Ciências e Tecnologia da Biomassa (ex-GDEH)RUNLeitão, Ana LúciaEnguita, Francisco J.2022-03-24T23:38:53Z2021-07-162021-07-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/135182eng2218-273XPURE: 42480076https://doi.org/10.3390/biom11071040info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:13:37Zoai:run.unl.pt:10362/135182Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:48:20.447332Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Systematic structure-based search for ochratoxin-degrading enzymes in proteomes from filamentous fungi
title Systematic structure-based search for ochratoxin-degrading enzymes in proteomes from filamentous fungi
spellingShingle Systematic structure-based search for ochratoxin-degrading enzymes in proteomes from filamentous fungi
Leitão, Ana Lúcia
Biodegradation
Mycotoxin
Ochratoxin A
Ochratoxinase
Structural biology
Biochemistry
Molecular Biology
title_short Systematic structure-based search for ochratoxin-degrading enzymes in proteomes from filamentous fungi
title_full Systematic structure-based search for ochratoxin-degrading enzymes in proteomes from filamentous fungi
title_fullStr Systematic structure-based search for ochratoxin-degrading enzymes in proteomes from filamentous fungi
title_full_unstemmed Systematic structure-based search for ochratoxin-degrading enzymes in proteomes from filamentous fungi
title_sort Systematic structure-based search for ochratoxin-degrading enzymes in proteomes from filamentous fungi
author Leitão, Ana Lúcia
author_facet Leitão, Ana Lúcia
Enguita, Francisco J.
author_role author
author2 Enguita, Francisco J.
author2_role author
dc.contributor.none.fl_str_mv MEtRICS - Centro de Engenharia Mecânica e Sustentabilidade de Recursos
DCTB - Departamento de Ciências e Tecnologia da Biomassa (ex-GDEH)
RUN
dc.contributor.author.fl_str_mv Leitão, Ana Lúcia
Enguita, Francisco J.
dc.subject.por.fl_str_mv Biodegradation
Mycotoxin
Ochratoxin A
Ochratoxinase
Structural biology
Biochemistry
Molecular Biology
topic Biodegradation
Mycotoxin
Ochratoxin A
Ochratoxinase
Structural biology
Biochemistry
Molecular Biology
description PTDC-MED-GEN-29389-2017
publishDate 2021
dc.date.none.fl_str_mv 2021-07-16
2021-07-16T00:00:00Z
2022-03-24T23:38:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/135182
url http://hdl.handle.net/10362/135182
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2218-273X
PURE: 42480076
https://doi.org/10.3390/biom11071040
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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