Spring variation of total sugars and phenols on woody plants important for Red and Roe deer in Montesinho Natural Park (NE Portugal)

Detalhes bibliográficos
Autor(a) principal: Haddouch, Imane
Data de Publicação: 2017
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/14774
Resumo: Red deer (Cervus elaphus) and Roe deer (Capreolus capreolus) are two ungulate species cohabite the same area in the Natural Park of Montesinho, this work aims to manage the study spring diet of both deer with exploring the importance of secondary metabolites on feeding selection by analysis phytochemical of three Mediterranean species Quercus rotundifolia, Pterospartum tridentatum, Cistus ladanifer. The collection of data was achieve during flowering period, from fives sites in the park. Phytochemical analysis based on extraction of total sugar by methanol-aqueous (80,20) reagent using DNS method, and the extraction of total Phenolic compounds TPC by hot methanol and folin-ciocalteu reagent and using spectrophotometer to read the absorbance of each sample. The quantification of sugar was use as a calibration curve the “glucose”, and for phenol we have used “acid Gallic”. The statistical analysis (Anova test, Tukey HSD) of all results obtained from the both extraction showed significative differences between plant species and between months and were used to compare between the choice of deer species on feeding during flowering period (March, April, June).Quercus rotundifolia showed highest phenol and sugar contents and seems to be avoided to consumption by deer. In contrast Pterospartum tridentatum showed an increase of sugar concentration and maintain phenolic contentsthat could explain the consumption during this period.
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spelling Spring variation of total sugars and phenols on woody plants important for Red and Roe deer in Montesinho Natural Park (NE Portugal)Capreolus capreolusCervus elaphusCistus ladaniferPterospartum tridentatumQuercus rotundifoliaTotal sugarDomínio/Área Científica::Ciências Agrárias::Agricultura, Silvicultura e PescasRed deer (Cervus elaphus) and Roe deer (Capreolus capreolus) are two ungulate species cohabite the same area in the Natural Park of Montesinho, this work aims to manage the study spring diet of both deer with exploring the importance of secondary metabolites on feeding selection by analysis phytochemical of three Mediterranean species Quercus rotundifolia, Pterospartum tridentatum, Cistus ladanifer. The collection of data was achieve during flowering period, from fives sites in the park. Phytochemical analysis based on extraction of total sugar by methanol-aqueous (80,20) reagent using DNS method, and the extraction of total Phenolic compounds TPC by hot methanol and folin-ciocalteu reagent and using spectrophotometer to read the absorbance of each sample. The quantification of sugar was use as a calibration curve the “glucose”, and for phenol we have used “acid Gallic”. The statistical analysis (Anova test, Tukey HSD) of all results obtained from the both extraction showed significative differences between plant species and between months and were used to compare between the choice of deer species on feeding during flowering period (March, April, June).Quercus rotundifolia showed highest phenol and sugar contents and seems to be avoided to consumption by deer. In contrast Pterospartum tridentatum showed an increase of sugar concentration and maintain phenolic contentsthat could explain the consumption during this period.O veado (Cervus elaphus) e o Corço (Capreolus capreolus) são duas espécies unguladas que coabitam a mesma área no parque natural de Montesinho, este trabalho tem como objetivo gerenciar a dieta de primavera do estudo de ambos os veados com a análise da importância dos metabolitos secundários na seleção de alimentação Por análise fitoquímica de três espécies mediterrâneas "Quercus rotundifolia", "Pterospartum tridentatum", "Cistus ladanifer". A coleta de dados foi alcançada durante o período de floração, a partir de cinco locais no parque. Análise fitoquímica baseada na extração de açúcar total por reagente metanol-aquoso (80,20) usando o método DNS e extração de compostos fenólicos TPC por metanol quente e reagente folin-ciocalteu e utilizando espectrofotômetro para ler a absorvância de cada amostra. A quantificação de açúcar foi utilizada como curva de calibração, a "glicose", e para fenol utilizamos "ácido galo". A análise estatística (teste Anova, Tukey HSD) de todos os resultados obtidos a partir das duas extrações mostrou diferenças significativas entre as espécies vegetais e entre meses e foram utilizadas para comparar a escolha dos cervídeos na alimentação, durante o período de floração (março, abril, junho). Quercus rotundifolia apresentou maior teor de fenol e açúcar e parece ser evitado para o consumo por veado. Em contraste, Pterospartum tridentatum mostrou um aumento da concentração de açúcar e manteve conteúdo fenólico, o que poderia explicar o consumo durante este período.Le cerf-élaphe(Cervus elaphus) et le chevreuil (Capreolus capreolus) sont deux espèces d'ongulés cohabitent dans la même région du parc naturel de Montesinho, ce travail vise à gérer l'étude du régime alimentaire des deux cerfs en explorant l'importance des métabolites secondaires lors de la sélection de l'alimentation Par analyse phytochimique de trois espèces méditerranéennes "Quercus rotundifolia", "Pterospartum tridentatum", "Cistus ladanifer". La collecte des données a été réalisée pendant la période de floraison, à partir des cinq sites du parc. Analyse phytochimique basée sur l'extraction du sucre total par le réacteur méthanol-aqueux (80,20) utilisant la méthode DNS et l'extraction des composés phénoliques totaux TPC par le réacteur chaud au méthanol et au folin-ciocalteu et en utilisant un spectrophotomètre pour lire l'absorbance de chaque échantillon. La quantification du sucre a été utilisée comme courbe d'étalonnage du «glucose», et pour le phénol, nous avons utilisé «acide gallique». L'analyse statistique (test Anova, Tukey HSD) de tous les résultats obtenus à partir des deux extractions a montré des différences significatives entre les espèces végétales et entre les mois et ont été utilisées pour comparer le choix des espèces de cerf sur l'alimentation pendant la période de floraison (mars, avril et juin). Quercus rotundifolia a affiché des teneurs en phénol et en sucre les plus élevées et semble être évité par la consommation chez les cerfs. En revanche, Pterospartum tridentatum a montré une augmentation de la concentration de sucre et de maintenir un contenu phénolique qui pourrait expliquer la consommation pendant cette période.Cortez, José PauloElayachi, SehharBiblioteca Digital do IPBHaddouch, Imane2017-12-18T17:26:10Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10198/14774TID:201788748enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:34:37Zoai:bibliotecadigital.ipb.pt:10198/14774Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:04:32.072911Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Spring variation of total sugars and phenols on woody plants important for Red and Roe deer in Montesinho Natural Park (NE Portugal)
title Spring variation of total sugars and phenols on woody plants important for Red and Roe deer in Montesinho Natural Park (NE Portugal)
spellingShingle Spring variation of total sugars and phenols on woody plants important for Red and Roe deer in Montesinho Natural Park (NE Portugal)
Haddouch, Imane
Capreolus capreolus
Cervus elaphus
Cistus ladanifer
Pterospartum tridentatum
Quercus rotundifolia
Total sugar
Domínio/Área Científica::Ciências Agrárias::Agricultura, Silvicultura e Pescas
title_short Spring variation of total sugars and phenols on woody plants important for Red and Roe deer in Montesinho Natural Park (NE Portugal)
title_full Spring variation of total sugars and phenols on woody plants important for Red and Roe deer in Montesinho Natural Park (NE Portugal)
title_fullStr Spring variation of total sugars and phenols on woody plants important for Red and Roe deer in Montesinho Natural Park (NE Portugal)
title_full_unstemmed Spring variation of total sugars and phenols on woody plants important for Red and Roe deer in Montesinho Natural Park (NE Portugal)
title_sort Spring variation of total sugars and phenols on woody plants important for Red and Roe deer in Montesinho Natural Park (NE Portugal)
author Haddouch, Imane
author_facet Haddouch, Imane
author_role author
dc.contributor.none.fl_str_mv Cortez, José Paulo
Elayachi, Sehhar
Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Haddouch, Imane
dc.subject.por.fl_str_mv Capreolus capreolus
Cervus elaphus
Cistus ladanifer
Pterospartum tridentatum
Quercus rotundifolia
Total sugar
Domínio/Área Científica::Ciências Agrárias::Agricultura, Silvicultura e Pescas
topic Capreolus capreolus
Cervus elaphus
Cistus ladanifer
Pterospartum tridentatum
Quercus rotundifolia
Total sugar
Domínio/Área Científica::Ciências Agrárias::Agricultura, Silvicultura e Pescas
description Red deer (Cervus elaphus) and Roe deer (Capreolus capreolus) are two ungulate species cohabite the same area in the Natural Park of Montesinho, this work aims to manage the study spring diet of both deer with exploring the importance of secondary metabolites on feeding selection by analysis phytochemical of three Mediterranean species Quercus rotundifolia, Pterospartum tridentatum, Cistus ladanifer. The collection of data was achieve during flowering period, from fives sites in the park. Phytochemical analysis based on extraction of total sugar by methanol-aqueous (80,20) reagent using DNS method, and the extraction of total Phenolic compounds TPC by hot methanol and folin-ciocalteu reagent and using spectrophotometer to read the absorbance of each sample. The quantification of sugar was use as a calibration curve the “glucose”, and for phenol we have used “acid Gallic”. The statistical analysis (Anova test, Tukey HSD) of all results obtained from the both extraction showed significative differences between plant species and between months and were used to compare between the choice of deer species on feeding during flowering period (March, April, June).Quercus rotundifolia showed highest phenol and sugar contents and seems to be avoided to consumption by deer. In contrast Pterospartum tridentatum showed an increase of sugar concentration and maintain phenolic contentsthat could explain the consumption during this period.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-18T17:26:10Z
2017
2017-01-01T00:00:00Z
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