Manufacture of β-TCP/alginate scaffolds through a Fab@home model for application in bone tissue engineering
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.6/4651 |
Resumo: | The growing need to treat bone-related diseases in an elderly population compels the development of novel bone substitutes to improve patient quality of life. In this context, the advent of affordable and effective rapid prototyping equipment, such as the Fab@home plotter, has contributed to the development of novel scaffolds for bone tissue engineering. In this study, we report for the first time the use of a Fab@home plotter for the production of 3D scaffolds composed by beta-tricalcium phosphate (β-TCP)/alginate hybrid materials. β-TCP/alginate mixtures were used in a proportion of 50/50% (w/w), 30/70% (w/w) and 20/80% (w/w). The printing parameters were optimized to a nozzle diameter of 20 Gauge for the production of rigid scaffolds with pre-defined architectures. We observed that, despite using similar printing parameters, both the precision and resolution of the scaffolds were significantly affected by the blend's viscosity. In particular, we demonstrate that the higher viscosity of 50/50 scaffolds (150.0 ± 3.91 mPa s) provides a higher precision in the extrusion process. The physicochemical and biological characterization of the samples demonstrated that the 50/50 scaffolds possessed a resistance to compression comparable to that of native trabecular bone. Moreover, this particular formulation also exhibited a Young's modulus that was higher than that of trabecular bone. Scanning electron microscopy and fluorescence microscopy analysis revealed that osteoblasts were able to adhere, proliferate and also penetrate into the scaffold's architecture. Altogether, our findings suggest that the Fab@home printer can be employed in the manufacture of reproducible scaffolds, using a formulation 50/50 alginate-β-TCP that has suitable properties to be applied as bone substitutes in the future. |
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Manufacture of β-TCP/alginate scaffolds through a Fab@home model for application in bone tissue engineeringRapid prototypingBone tissue engineeringBiopolymers3D plotterHybrid materialsThe growing need to treat bone-related diseases in an elderly population compels the development of novel bone substitutes to improve patient quality of life. In this context, the advent of affordable and effective rapid prototyping equipment, such as the Fab@home plotter, has contributed to the development of novel scaffolds for bone tissue engineering. In this study, we report for the first time the use of a Fab@home plotter for the production of 3D scaffolds composed by beta-tricalcium phosphate (β-TCP)/alginate hybrid materials. β-TCP/alginate mixtures were used in a proportion of 50/50% (w/w), 30/70% (w/w) and 20/80% (w/w). The printing parameters were optimized to a nozzle diameter of 20 Gauge for the production of rigid scaffolds with pre-defined architectures. We observed that, despite using similar printing parameters, both the precision and resolution of the scaffolds were significantly affected by the blend's viscosity. In particular, we demonstrate that the higher viscosity of 50/50 scaffolds (150.0 ± 3.91 mPa s) provides a higher precision in the extrusion process. The physicochemical and biological characterization of the samples demonstrated that the 50/50 scaffolds possessed a resistance to compression comparable to that of native trabecular bone. Moreover, this particular formulation also exhibited a Young's modulus that was higher than that of trabecular bone. Scanning electron microscopy and fluorescence microscopy analysis revealed that osteoblasts were able to adhere, proliferate and also penetrate into the scaffold's architecture. Altogether, our findings suggest that the Fab@home printer can be employed in the manufacture of reproducible scaffolds, using a formulation 50/50 alginate-β-TCP that has suitable properties to be applied as bone substitutes in the future.IOP PublishinguBibliorumCarlos, Gabriela Soares DiogoGaspar, Vítor Manuel AbreuSerra, Inês Raquel TavaresFradique, Ricardo GilCorreia, Ilídio Joaquim Sobreira2018-03-20T10:40:17Z2014-03-212014-03-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4651engDiogo, G.S., Gaspar, V.M., Serra, I.R., Fradique, R. e Correia, I.J. (2014) "Manufacture of β-TCP/Alginate scaffolds through a Fab@home model for application in bone tissue engineering", Biofabrication, Vol. 6 (2), pp. 02500110.1088/1758-5082/6/2/025001metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-20T02:30:32Zoai:ubibliorum.ubi.pt:10400.6/4651Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:45:42.667057Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Manufacture of β-TCP/alginate scaffolds through a Fab@home model for application in bone tissue engineering |
title |
Manufacture of β-TCP/alginate scaffolds through a Fab@home model for application in bone tissue engineering |
spellingShingle |
Manufacture of β-TCP/alginate scaffolds through a Fab@home model for application in bone tissue engineering Carlos, Gabriela Soares Diogo Rapid prototyping Bone tissue engineering Biopolymers 3D plotter Hybrid materials |
title_short |
Manufacture of β-TCP/alginate scaffolds through a Fab@home model for application in bone tissue engineering |
title_full |
Manufacture of β-TCP/alginate scaffolds through a Fab@home model for application in bone tissue engineering |
title_fullStr |
Manufacture of β-TCP/alginate scaffolds through a Fab@home model for application in bone tissue engineering |
title_full_unstemmed |
Manufacture of β-TCP/alginate scaffolds through a Fab@home model for application in bone tissue engineering |
title_sort |
Manufacture of β-TCP/alginate scaffolds through a Fab@home model for application in bone tissue engineering |
author |
Carlos, Gabriela Soares Diogo |
author_facet |
Carlos, Gabriela Soares Diogo Gaspar, Vítor Manuel Abreu Serra, Inês Raquel Tavares Fradique, Ricardo Gil Correia, Ilídio Joaquim Sobreira |
author_role |
author |
author2 |
Gaspar, Vítor Manuel Abreu Serra, Inês Raquel Tavares Fradique, Ricardo Gil Correia, Ilídio Joaquim Sobreira |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
uBibliorum |
dc.contributor.author.fl_str_mv |
Carlos, Gabriela Soares Diogo Gaspar, Vítor Manuel Abreu Serra, Inês Raquel Tavares Fradique, Ricardo Gil Correia, Ilídio Joaquim Sobreira |
dc.subject.por.fl_str_mv |
Rapid prototyping Bone tissue engineering Biopolymers 3D plotter Hybrid materials |
topic |
Rapid prototyping Bone tissue engineering Biopolymers 3D plotter Hybrid materials |
description |
The growing need to treat bone-related diseases in an elderly population compels the development of novel bone substitutes to improve patient quality of life. In this context, the advent of affordable and effective rapid prototyping equipment, such as the Fab@home plotter, has contributed to the development of novel scaffolds for bone tissue engineering. In this study, we report for the first time the use of a Fab@home plotter for the production of 3D scaffolds composed by beta-tricalcium phosphate (β-TCP)/alginate hybrid materials. β-TCP/alginate mixtures were used in a proportion of 50/50% (w/w), 30/70% (w/w) and 20/80% (w/w). The printing parameters were optimized to a nozzle diameter of 20 Gauge for the production of rigid scaffolds with pre-defined architectures. We observed that, despite using similar printing parameters, both the precision and resolution of the scaffolds were significantly affected by the blend's viscosity. In particular, we demonstrate that the higher viscosity of 50/50 scaffolds (150.0 ± 3.91 mPa s) provides a higher precision in the extrusion process. The physicochemical and biological characterization of the samples demonstrated that the 50/50 scaffolds possessed a resistance to compression comparable to that of native trabecular bone. Moreover, this particular formulation also exhibited a Young's modulus that was higher than that of trabecular bone. Scanning electron microscopy and fluorescence microscopy analysis revealed that osteoblasts were able to adhere, proliferate and also penetrate into the scaffold's architecture. Altogether, our findings suggest that the Fab@home printer can be employed in the manufacture of reproducible scaffolds, using a formulation 50/50 alginate-β-TCP that has suitable properties to be applied as bone substitutes in the future. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-03-21 2014-03-21T00:00:00Z 2018-03-20T10:40:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.6/4651 |
url |
http://hdl.handle.net/10400.6/4651 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Diogo, G.S., Gaspar, V.M., Serra, I.R., Fradique, R. e Correia, I.J. (2014) "Manufacture of β-TCP/Alginate scaffolds through a Fab@home model for application in bone tissue engineering", Biofabrication, Vol. 6 (2), pp. 025001 10.1088/1758-5082/6/2/025001 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
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metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
IOP Publishing |
publisher.none.fl_str_mv |
IOP Publishing |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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