Diverse specificity of cellulosome attachment to the bacterial cell surface
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/36982 |
Resumo: | This work was supported by the EU FP7 programme under the WallTraC project (grant No. 263916) and by projects PTDC/BIA-MIC/5947/2014, RECI/BBB-BEP/0124/2012 and EXPL/BIA-MIC/1176/2012 supported by Fundacao para a Ciencia e Tecnologia (FCT-MCTES). The Research Unit UCIBIO (Unidade de Ciencias Biomoleculares Aplicadas) is financed by national funds from FCT/MCTES EC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). We thank the European Synchrotron Radiation Facility (Grenoble, France), Soleil (Saint-Aubin, France) and Diamond Light Source (Harwell, UK) for data collection and the European Community's Seventh Framework Programme (FP7/2007-2013) under BioStruct-X (grant agreement No. 283570, proposal number: Biostruct-X_ 4399) for funding. |
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Diverse specificity of cellulosome attachment to the bacterial cell surfaceCOHESIN-DOCKERIN COMPLEXDUAL BINDING MODECLOSTRIDIUM-THERMOCELLUMCRYSTAL-STRUCTUREMODULESDOMAINThis work was supported by the EU FP7 programme under the WallTraC project (grant No. 263916) and by projects PTDC/BIA-MIC/5947/2014, RECI/BBB-BEP/0124/2012 and EXPL/BIA-MIC/1176/2012 supported by Fundacao para a Ciencia e Tecnologia (FCT-MCTES). The Research Unit UCIBIO (Unidade de Ciencias Biomoleculares Aplicadas) is financed by national funds from FCT/MCTES EC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). We thank the European Synchrotron Radiation Facility (Grenoble, France), Soleil (Saint-Aubin, France) and Diamond Light Source (Harwell, UK) for data collection and the European Community's Seventh Framework Programme (FP7/2007-2013) under BioStruct-X (grant agreement No. 283570, proposal number: Biostruct-X_ 4399) for funding.During the course of evolution, the cellulosome, one of Nature's most intricate multi-enzyme complexes, has been continuously fine-tuned to efficiently deconstruct recalcitrant carbohydrates. To facilitate the uptake of released sugars, anaerobic bacteria use highly ordered protein-protein interactions to recruit these nanomachines to the cell surface. Dockerin modules located within a non-catalytic macromolecular scaffold, whose primary role is to assemble cellulosomal enzymatic subunits, bind cohesin modules of cell envelope proteins, thereby anchoring the cellulosome onto the bacterial cell. Here we have elucidated the unique molecular mechanisms used by anaerobic bacteria for cellulosome cellular attachment. The structure and biochemical analysis of five cohesin-dockerin complexes revealed that cell surface dockerins contain two cohesin-binding interfaces, which can present different or identical specificities. In contrast to the current static model, we propose that dockerins utilize multivalent modes of cohesin recognition to recruit cellulosomes to the cell surface, a mechanism that maximises substrate access while facilitating complex assembly.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNBrás, Joana L. A.Pinheiro, Benedita A.Cameron, KateCuskin, FionaViegas, AldinoNajmudin, ShabirBule, PedroPires, Virginia M. R.Romão, Maria JoãoBayer, Edward A.Spencer, Holly LSmith, Steven PGilbert, Harry J.Alves, Victor DCarvalho, Ana LuísaFontes, Carlos M. G. A.2018-05-14T22:04:35Z2016-12-072016-12-07T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://hdl.handle.net/10362/36982eng2045-2322PURE: 2517978https://doi.org/10.1038/srep38292info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:20:32Zoai:run.unl.pt:10362/36982Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:44.342233Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Diverse specificity of cellulosome attachment to the bacterial cell surface |
| title |
Diverse specificity of cellulosome attachment to the bacterial cell surface |
| spellingShingle |
Diverse specificity of cellulosome attachment to the bacterial cell surface Brás, Joana L. A. COHESIN-DOCKERIN COMPLEX DUAL BINDING MODE CLOSTRIDIUM-THERMOCELLUM CRYSTAL-STRUCTURE MODULES DOMAIN |
| title_short |
Diverse specificity of cellulosome attachment to the bacterial cell surface |
| title_full |
Diverse specificity of cellulosome attachment to the bacterial cell surface |
| title_fullStr |
Diverse specificity of cellulosome attachment to the bacterial cell surface |
| title_full_unstemmed |
Diverse specificity of cellulosome attachment to the bacterial cell surface |
| title_sort |
Diverse specificity of cellulosome attachment to the bacterial cell surface |
| author |
Brás, Joana L. A. |
| author_facet |
Brás, Joana L. A. Pinheiro, Benedita A. Cameron, Kate Cuskin, Fiona Viegas, Aldino Najmudin, Shabir Bule, Pedro Pires, Virginia M. R. Romão, Maria João Bayer, Edward A. Spencer, Holly L Smith, Steven P Gilbert, Harry J. Alves, Victor D Carvalho, Ana Luísa Fontes, Carlos M. G. A. |
| author_role |
author |
| author2 |
Pinheiro, Benedita A. Cameron, Kate Cuskin, Fiona Viegas, Aldino Najmudin, Shabir Bule, Pedro Pires, Virginia M. R. Romão, Maria João Bayer, Edward A. Spencer, Holly L Smith, Steven P Gilbert, Harry J. Alves, Victor D Carvalho, Ana Luísa Fontes, Carlos M. G. A. |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
UCIBIO - Applied Molecular Biosciences Unit DQ - Departamento de Química RUN |
| dc.contributor.author.fl_str_mv |
Brás, Joana L. A. Pinheiro, Benedita A. Cameron, Kate Cuskin, Fiona Viegas, Aldino Najmudin, Shabir Bule, Pedro Pires, Virginia M. R. Romão, Maria João Bayer, Edward A. Spencer, Holly L Smith, Steven P Gilbert, Harry J. Alves, Victor D Carvalho, Ana Luísa Fontes, Carlos M. G. A. |
| dc.subject.por.fl_str_mv |
COHESIN-DOCKERIN COMPLEX DUAL BINDING MODE CLOSTRIDIUM-THERMOCELLUM CRYSTAL-STRUCTURE MODULES DOMAIN |
| topic |
COHESIN-DOCKERIN COMPLEX DUAL BINDING MODE CLOSTRIDIUM-THERMOCELLUM CRYSTAL-STRUCTURE MODULES DOMAIN |
| description |
This work was supported by the EU FP7 programme under the WallTraC project (grant No. 263916) and by projects PTDC/BIA-MIC/5947/2014, RECI/BBB-BEP/0124/2012 and EXPL/BIA-MIC/1176/2012 supported by Fundacao para a Ciencia e Tecnologia (FCT-MCTES). The Research Unit UCIBIO (Unidade de Ciencias Biomoleculares Aplicadas) is financed by national funds from FCT/MCTES EC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). We thank the European Synchrotron Radiation Facility (Grenoble, France), Soleil (Saint-Aubin, France) and Diamond Light Source (Harwell, UK) for data collection and the European Community's Seventh Framework Programme (FP7/2007-2013) under BioStruct-X (grant agreement No. 283570, proposal number: Biostruct-X_ 4399) for funding. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016-12-07 2016-12-07T00:00:00Z 2018-05-14T22:04:35Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/36982 |
| url |
http://hdl.handle.net/10362/36982 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2045-2322 PURE: 2517978 https://doi.org/10.1038/srep38292 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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12 application/pdf |
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