Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/4085 |
Resumo: | A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the complex formation between CDs, Na+ , cholesterol and DHB, and it was supported by theoretical calcula tions. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+. |
id |
RCAP_10f1730709a1901103aac886bb227a6e |
---|---|
oai_identifier_str |
oai:digituma.uma.pt:10400.13/4085 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometryCholesterolMALDI TOFCarbon dotsN-doped Carbon dotsDFT calculations.Faculdade de Ciências Exatas e da EngenhariaCentro de Química da MadeiraA binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the complex formation between CDs, Na+ , cholesterol and DHB, and it was supported by theoretical calcula tions. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+.ElsevierDigitUMaHoudová, DominikaSoto, JuanCastro, RitaRodrigues, JoãoSoledad Pino-González, MªPetković, MarijanaBandosz, Teresa JAlgarra, Manuel2022-02-16T15:40:53Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/4085engHoudová, D., Soto, J., Castro, R., Rodrigues, J., Pino-González, M. S., Petković, M., ... & Algarra, M. (2021). Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry. Journal of Colloid and Interface Science, 591, 373-383. https://doi.org/10.1016/j.jcis.2021.02.00410.1016/j.jcis.2021.02.004info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-10T06:27:11Zoai:digituma.uma.pt:10400.13/4085Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:07:54.608307Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry |
title |
Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry |
spellingShingle |
Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry Houdová, Dominika Cholesterol MALDI TOF Carbon dots N-doped Carbon dots DFT calculations . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira |
title_short |
Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry |
title_full |
Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry |
title_fullStr |
Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry |
title_full_unstemmed |
Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry |
title_sort |
Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry |
author |
Houdová, Dominika |
author_facet |
Houdová, Dominika Soto, Juan Castro, Rita Rodrigues, João Soledad Pino-González, Mª Petković, Marijana Bandosz, Teresa J Algarra, Manuel |
author_role |
author |
author2 |
Soto, Juan Castro, Rita Rodrigues, João Soledad Pino-González, Mª Petković, Marijana Bandosz, Teresa J Algarra, Manuel |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
DigitUMa |
dc.contributor.author.fl_str_mv |
Houdová, Dominika Soto, Juan Castro, Rita Rodrigues, João Soledad Pino-González, Mª Petković, Marijana Bandosz, Teresa J Algarra, Manuel |
dc.subject.por.fl_str_mv |
Cholesterol MALDI TOF Carbon dots N-doped Carbon dots DFT calculations . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira |
topic |
Cholesterol MALDI TOF Carbon dots N-doped Carbon dots DFT calculations . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira |
description |
A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the complex formation between CDs, Na+ , cholesterol and DHB, and it was supported by theoretical calcula tions. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2021-01-01T00:00:00Z 2022-02-16T15:40:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/4085 |
url |
http://hdl.handle.net/10400.13/4085 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Houdová, D., Soto, J., Castro, R., Rodrigues, J., Pino-González, M. S., Petković, M., ... & Algarra, M. (2021). Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry. Journal of Colloid and Interface Science, 591, 373-383. https://doi.org/10.1016/j.jcis.2021.02.004 10.1016/j.jcis.2021.02.004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799129947529281536 |